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Q27543 (PFKA_CALFI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:PFK
OrganismCalanus finmarchicus (Calanus tonsus)
Taxonomic identifier6837 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMaxillopodaCopepodaCalanoidaCalanidaeCalanus

Protein attributes

Sequence length184 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactor

Magnesium By similarity.

Enzyme regulation

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termAllosteric enzyme
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›184›184ATP-dependent 6-phosphofructokinase
PRO_0000112028

Regions

Nucleotide binding2 – 54ATP By similarity
Region‹1 – ›184›184N-terminal catalytic PFK domain 1
Region48 – 503Substrate binding By similarity
Region92 – 943Substrate binding By similarity
Region182 – ›184›3Substrate binding By similarity

Sites

Active site501Proton acceptor By similarity
Metal binding31Magnesium; catalytic By similarity
Binding site851Substrate; shared with dimeric partner By similarity
Binding site1481Substrate By similarity
Binding site1761Substrate; shared with dimeric partner By similarity

Experimental info

Non-terminal residue11
Non-terminal residue1841

Sequences

Sequence LengthMass (Da)Tools
Q27543 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E9B71F095A5DF2AD

FASTA18420,208
        10         20         30         40         50         60 
GGDGSLTGAN RFKGEWSSLV KELLETGKIT KEVAEKHSHL NIVGMVGSID NDFCGTDMTI 

        70         80         90        100        110        120 
GTDSALHRIV EVADNIIPTA YSHQRAFVLE VMGRHCGYLA LVAGIVTEAD FVFAPEWPPE 

       130        140        150        160        170        180 
EDWPEKLCKK LELERQSGQR LNIIIVAEGA IDRQGNPITA EGVKKIIVDR LEMDTRTTVL 


GHIQ 

« Hide

References

[1]"Nuclear genes from the copepod Calanus finmarchicus."
Crawford D.L.
Mol. Mar. Biol. Biotechnol. 4:241-247(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U21244 mRNA. Translation: AAA85287.1.

3D structure databases

ProteinModelPortalQ27543.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

InterProIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA_CALFI
AccessionPrimary (citable) accession number: Q27543
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways