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Q27543

- PFKA_CALFI

UniProt

Q27543 - PFKA_CALFI

Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Calanus finmarchicus (Calanus tonsus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi3 – 31Magnesium; catalyticBy similarity
    Active sitei50 – 501Proton acceptorBy similarity
    Binding sitei85 – 851Substrate; shared with dimeric partnerBy similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Binding sitei176 – 1761Substrate; shared with dimeric partnerBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi2 – 54ATPBy similarity

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
    Short name:
    ATP-PFK
    Short name:
    Phosphofructokinase
    Alternative name(s):
    Phosphohexokinase
    Gene namesi
    Name:PFK
    OrganismiCalanus finmarchicus (Calanus tonsus)
    Taxonomic identifieri6837 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMaxillopodaCopepodaCalanoidaCalanidaeCalanus

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›184›184ATP-dependent 6-phosphofructokinasePRO_0000112028Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ27543.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni‹1 – ›184›184N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni48 – 503Substrate bindingBy similarity
    Regioni92 – 943Substrate bindingBy similarity
    Regioni182 – ›184›3Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Q27543-1 [UniParc]FASTAAdd to Basket

    « Hide

    GGDGSLTGAN RFKGEWSSLV KELLETGKIT KEVAEKHSHL NIVGMVGSID    50
    NDFCGTDMTI GTDSALHRIV EVADNIIPTA YSHQRAFVLE VMGRHCGYLA 100
    LVAGIVTEAD FVFAPEWPPE EDWPEKLCKK LELERQSGQR LNIIIVAEGA 150
    IDRQGNPITA EGVKKIIVDR LEMDTRTTVL GHIQ 184
    Length:184
    Mass (Da):20,208
    Last modified:November 1, 1996 - v1
    Checksum:iE9B71F095A5DF2AD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei184 – 1841

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21244 mRNA. Translation: AAA85287.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21244 mRNA. Translation: AAA85287.1 .

    3D structure databases

    ProteinModelPortali Q27543.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    InterProi IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nuclear genes from the copepod Calanus finmarchicus."
      Crawford D.L.
      Mol. Mar. Biol. Biotechnol. 4:241-247(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiPFKA_CALFI
    AccessioniPrimary (citable) accession number: Q27543
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3