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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Calanus finmarchicus (Calanus tonsus)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI)
  3. ATP-dependent 6-phosphofructokinase (PFK)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3Magnesium; catalyticBy similarity1
Active sitei50Proton acceptorBy similarity1
Binding sitei85Substrate; shared with dimeric partnerBy similarity1
Binding sitei148SubstrateBy similarity1
Binding sitei176Substrate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi2 – 5ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:PFK
OrganismiCalanus finmarchicus (Calanus tonsus)
Taxonomic identifieri6837 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMulticrustaceaHexanaupliaCopepodaCalanoidaCalanidaeCalanus

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000112028‹1 – ›184ATP-dependent 6-phosphofructokinaseAdd BLAST›184

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ27543
SMRiQ27543
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni‹1 – ›184N-terminal catalytic PFK domain 1By similarityAdd BLAST›184
Regioni48 – 50Substrate bindingBy similarity3
Regioni92 – 94Substrate bindingBy similarity3
Regioni182 – ›184Substrate bindingBy similarity›3

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Fragment.

Q27543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GGDGSLTGAN RFKGEWSSLV KELLETGKIT KEVAEKHSHL NIVGMVGSID
60 70 80 90 100
NDFCGTDMTI GTDSALHRIV EVADNIIPTA YSHQRAFVLE VMGRHCGYLA
110 120 130 140 150
LVAGIVTEAD FVFAPEWPPE EDWPEKLCKK LELERQSGQR LNIIIVAEGA
160 170 180
IDRQGNPITA EGVKKIIVDR LEMDTRTTVL GHIQ
Length:184
Mass (Da):20,208
Last modified:November 1, 1996 - v1
Checksum:iE9B71F095A5DF2AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei1841

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21244 mRNA Translation: AAA85287.1

Entry informationi

Entry nameiPFKA_CALFI
AccessioniPrimary (citable) accession number: Q27543
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

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