Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q27541

- HPRT_CRIFA

UniProt

Q27541 - HPRT_CRIFA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HGPRT

Organism
Crithidia fasciculata
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631GMPBy similarity
Active sitei126 – 1261Proton acceptorBy similarity
Binding sitei154 – 1541GMPBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity
Binding sitei182 – 1821GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 1309GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HGPRT
OrganismiCrithidia fasciculata
Taxonomic identifieri5656 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeCrithidia

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139595Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ27541.
SMRiQ27541. Positions 17-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27541-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNAASPATS AAPVRHYPMS CRTLATQEQI WSATAKCAKQ IAEDYKQYNL
60 70 80 90 100
SDENPLYLLC VLKGSFMFTA DLARFLCDEG VPVRIEFICA SSYGTDVKTS
110 120 130 140 150
GEVRLLLDVR DPVENRHLLI VEDIVDSAIT LEYLKRFLQA KQPASLKTVV
160 170 180 190 200
LLDKPSGRKV TLSVDYPVIT IPHAFVIGYG MDFAEAYREL RDVCVLKKEY

YEKPASKL
Length:208
Mass (Da):23,323
Last modified:November 1, 1996 - v1
Checksum:i7205B52781372EE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19968 Genomic DNA. Translation: AAB04164.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19968 Genomic DNA. Translation: AAB04164.1 .

3D structure databases

ProteinModelPortali Q27541.
SMRi Q27541. Positions 17-198.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Crithidia fasciculata: isolation, sequencing, and expression of the hypoxanthine-guanine phosphoribosyltransferase gene."
    Jiang Y., Allen T.E., Carter D., Ray D.S., Ullman B.
    Exp. Parasitol. 82:73-75(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiHPRT_CRIFA
AccessioniPrimary (citable) accession number: Q27541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3