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Protein

Probable S-adenosylmethionine synthase 5

Gene

sams-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent ions per subunit. Magnesium or cobalt.By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable S-adenosylmethionine synthase 1 (sams-1), Probable S-adenosylmethionine synthase 3 (sams-3), Probable S-adenosylmethionine synthase 4 (sams-4), Probable S-adenosylmethionine synthase 5 (sams-5)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191MagnesiumBy similarity
Metal bindingi45 – 451PotassiumBy similarity
Binding sitei147 – 1471ATPSequence analysis
Metal bindingi271 – 2711PotassiumBy similarity
Metal bindingi279 – 2791MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi119 – 1246ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-CEL-156581. Methylation.
R-CEL-1614635. Sulfur amino acid metabolism.
R-CEL-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-adenosylmethionine synthase 5 (EC:2.5.1.6)
Short name:
AdoMet synthase 5
Alternative name(s):
Methionine adenosyltransferase 5
Short name:
MAT 5
Gene namesi
Name:sams-5
Synonyms:tag-32
ORF Names:T13A10.11
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiT13A10.11a; CE30175; WBGene00006416; sams-5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Probable S-adenosylmethionine synthase 5PRO_0000174445Add
BLAST

Proteomic databases

EPDiQ27522.
PaxDbiQ27522.
PRIDEiQ27522.

Interactioni

Protein-protein interaction databases

BioGridi42510. 1 interaction.
IntActiQ27522. 1 interaction.
STRINGi6239.T13A10.11a.1.

Structurei

3D structure databases

ProteinModelPortaliQ27522.
SMRiQ27522. Positions 6-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
InParanoidiQ27522.
KOiK00789.
OMAiSVQHDEC.
PhylomeDBiQ27522.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKNKFLFTS ESVSEGHPDK MCDQISDAVL DAHLAQDPHA KVACETVTKT
60 70 80 90 100
GMIMLCGEIT SKAVVDYQVL VRNVIKKIGY DDSSKGFDYK TCNVLVALEQ
110 120 130 140 150
QSPEIAAGVH VDKDIADVGA GDQGIMFGYA TDETEEAMPL TLLLSHKLNY
160 170 180 190 200
KLHELRRSGE LEWVRPDSKT QVTIEYSSEG GACVPLRVHT VVISTQHSPD
210 220 230 240 250
ISLEDLRKEL IEKVIKVVIP GNLIDNDTVY HLNPCGSFVV GGPMGDAGLT
260 270 280 290 300
GRKIIVDTYG GWGAHGGGAF SGKDPTKVDR SAAYAARWVA KSLVKAGLCR
310 320 330 340 350
RCLVQVSYAI GVAKPLSVMV FSFGTSALNE AELLKIVNDN FDLRPGMIIK
360 370 380 390 400
NLDLKRAIYE PTAENGHFGH NDFPWEQARK LKIDKDLRAK SKGPALVDAI

GIAH
Length:404
Mass (Da):43,949
Last modified:December 6, 2002 - v2
Checksum:i6411F0E567A04255
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081504 Genomic DNA. Translation: CCD72063.1.
PIRiT16856.
RefSeqiNP_741415.1. NM_171354.4.
UniGeneiCel.23406.

Genome annotation databases

EnsemblMetazoaiT13A10.11a.1; T13A10.11a.1; WBGene00006416.
T13A10.11a.2; T13A10.11a.2; WBGene00006416.
GeneIDi177389.
KEGGicel:CELE_T13A10.11.
UCSCiT13A10.11a.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081504 Genomic DNA. Translation: CCD72063.1.
PIRiT16856.
RefSeqiNP_741415.1. NM_171354.4.
UniGeneiCel.23406.

3D structure databases

ProteinModelPortaliQ27522.
SMRiQ27522. Positions 6-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi42510. 1 interaction.
IntActiQ27522. 1 interaction.
STRINGi6239.T13A10.11a.1.

Proteomic databases

EPDiQ27522.
PaxDbiQ27522.
PRIDEiQ27522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT13A10.11a.1; T13A10.11a.1; WBGene00006416.
T13A10.11a.2; T13A10.11a.2; WBGene00006416.
GeneIDi177389.
KEGGicel:CELE_T13A10.11.
UCSCiT13A10.11a.1. c. elegans.

Organism-specific databases

CTDi177389.
WormBaseiT13A10.11a; CE30175; WBGene00006416; sams-5.

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
InParanoidiQ27522.
KOiK00789.
OMAiSVQHDEC.
PhylomeDBiQ27522.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
ReactomeiR-CEL-156581. Methylation.
R-CEL-1614635. Sulfur amino acid metabolism.
R-CEL-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

NextBioi896598.
PROiQ27522.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiMETK5_CAEEL
AccessioniPrimary (citable) accession number: Q27522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 6, 2002
Last modified: May 11, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.