Reviewed, UniProtKB/Swiss-Prot Q27522 (METK5_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable S-adenosylmethionine synthetase 5
      Short name=AdoMet synthetase 5
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 5
      Short name=MAT 5

Gene names

Name:	sams-5
Synonyms:	tag-32
ORF Names:	T13A10.11

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q27522-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q27522-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Probable S-adenosylmethionine synthetase 5		PRO_0000174445
Regions
	Nucleotide binding	119 – 124	6	ATP Potential
Sites
	Metal binding	19	1	Magnesium By similarity
	Metal binding	45	1	Potassium By similarity
	Metal binding	271	1	Potassium By similarity
	Metal binding	279	1	Magnesium By similarity
	Binding site	147	1	ATP Potential
Natural variations
	Alternative sequence	1 – 51	51	Missing in isoform b.		VSP_005960

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 6411F0E567A04255	FASTA	404	43,949
	10 20 30 40 50 60 MSKNKFLFTS ESVSEGHPDK MCDQISDAVL DAHLAQDPHA KVACETVTKT GMIMLCGEIT 70 80 90 100 110 120 SKAVVDYQVL VRNVIKKIGY DDSSKGFDYK TCNVLVALEQ QSPEIAAGVH VDKDIADVGA 130 140 150 160 170 180 GDQGIMFGYA TDETEEAMPL TLLLSHKLNY KLHELRRSGE LEWVRPDSKT QVTIEYSSEG 190 200 210 220 230 240 GACVPLRVHT VVISTQHSPD ISLEDLRKEL IEKVIKVVIP GNLIDNDTVY HLNPCGSFVV 250 260 270 280 290 300 GGPMGDAGLT GRKIIVDTYG GWGAHGGGAF SGKDPTKVDR SAAYAARWVA KSLVKAGLCR 310 320 330 340 350 360 RCLVQVSYAI GVAKPLSVMV FSFGTSALNE AELLKIVNDN FDLRPGMIIK NLDLKRAIYE 370 380 390 400 PTAENGHFGH NDFPWEQARK LKIDKDLRAK SKGPALVDAI GIAH
	Isoform b. Checksum: 27A8A99DE6FE2FA7	FASTA	353	38,448
	10 20 30 40 50 60 MIMLCGEITS KAVVDYQVLV RNVIKKIGYD DSSKGFDYKT CNVLVALEQQ SPEIAAGVHV 70 80 90 100 110 120 DKDIADVGAG DQGIMFGYAT DETEEAMPLT LLLSHKLNYK LHELRRSGEL EWVRPDSKTQ 130 140 150 160 170 180 VTIEYSSEGG ACVPLRVHTV VISTQHSPDI SLEDLRKELI EKVIKVVIPG NLIDNDTVYH 190 200 210 220 230 240 LNPCGSFVVG GPMGDAGLTG RKIIVDTYGG WGAHGGGAFS GKDPTKVDRS AAYAARWVAK 250 260 270 280 290 300 SLVKAGLCRR CLVQVSYAIG VAKPLSVMVF SFGTSALNEA ELLKIVNDNF DLRPGMIIKN 310 320 330 340 350 LDLKRAIYEP TAENGHFGHN DFPWEQARKL KIDKDLRAKS KGPALVDAIG IAH

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U56963 Genomic DNA. Translation: AAB38126.2. U56963 Genomic DNA. Translation: AAM97949.1.
PIR	T16856.
RefSeq	NP_741415.1. NP_741416.1.
UniGene	Cel.17523 Cel.23406
3D structure databases
HSSP	HSSP built from PDB template 1QM4 based on UniProtKB P13444.
SMR	Q27522. Positions 6-382.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q27522. 1 interaction.
STRING	Q27522.
Proteomic databases
PRIDE	Q27522.
Genome annotation databases
Ensembl	T13A10.11a.1; T13A10.11a.1; T13A10.11; Caenorhabditis elegans. [Genome view] T13A10.11a.2; T13A10.11a.2; T13A10.11; Caenorhabditis elegans. [Genome view] T13A10.11b.1; T13A10.11b.1; T13A10.11; Caenorhabditis elegans. [Genome view] T13A10.11b.2; T13A10.11b.2; T13A10.11; Caenorhabditis elegans. [Genome view] T13A10.11b.3; T13A10.11b.3; T13A10.11; Caenorhabditis elegans. [Genome view]
GeneID	177389.
KEGG	cel:T13A10.11.
UCSC	T13A10.11a.1. c. elegans.
Organism-specific databases
CTD	177389.
WormBase	WBGene00006416. sams-5.
WormPep	T13A10.11a. CE30175. [WorfDB] T13A10.11b. CE31726. [WorfDB]
Phylogenomic databases
OMA	DQVIAPE.
Enzyme and pathway databases
BRENDA	2.5.1.6. 672.
Gene expression databases
ArrayExpress	Q27522.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	896598.

Entry information

Entry name

METK5_CAEEL

Accession

Primary (citable) accession number: Q27522

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 6, 2002
Last modified:	November 3, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project