Q27497 (GLC7A_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1-alpha
EC=3.1.3.16

Alternative name(s):
CeGLC-7-alpha
Glc seven-like phosphatase 1

Gene names

Name:	gsp-1
ORF Names:	F29F11.6

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine/threonine-protein phosphatase which antagonizes the function of air-2 in the regulation of chromosome cohesion. Dephosphorylates histone H3 at 'Ser-10'. Also involved in the activation of chloride channel clh-3 during cell swelling and meiotic maturation. Ref.2 Ref.3 Ref.4 Ref.5
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Ligand	Iron Manganese Metal-binding
Molecular function	Chromatin regulator Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Serine/threonine-protein phosphatase PP1-alpha		PRO_0000268637
Sites
	Active site	125	1	Proton donor By similarity
	Metal binding	64	1	Iron By similarity
	Metal binding	66	1	Iron By similarity
	Metal binding	92	1	Iron By similarity
	Metal binding	92	1	Manganese By similarity
	Metal binding	124	1	Manganese By similarity
	Metal binding	173	1	Manganese By similarity
	Metal binding	248	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27497 [UniParc]. Last modified January 1, 1999. Version 2. Checksum: 500145324CA16D1B	FASTA	329	37,205
	10 20 30 40 50 60 MSNDGDLNID NLITRLLEVR GCRPGKPVTM SEAEIRALCH KSREIFLSQP ILLELEAPLK 70 80 90 100 110 120 ICGDIHGQYN DLLRLFEYGG FPPEANYLFL GDYVDRGKQS LETICLLLAY KVKYPENFFL 130 140 150 160 170 180 LRGNHECASI NRIYGFYDEC KRRFSIKLWK TFTDCFNCLP IAALIDEKIF CCHGGLSPDL 190 200 210 220 230 240 QNMEQIRRVM RPTDVPDTGL LCDLLWSDPD KDVTGWGEND RGVSFTFGPD VVAKFLNRHD 250 260 270 280 290 300 LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG GMMSVDETLM CSFQILKPSE 310 320 KKAKYQYQGM NSGRPAVGGG RPGTTAGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes." Hsu J.-Y., Sun Z.-W., Li X., Reuben M., Tatchell K., Bishop D.K., Grushcow J.M., Brame C.J., Caldwell J.A., Hunt D.F., Lin R., Smith M.M., Allis C.D. Cell 102:279-291(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis." Rogers E., Bishop J.D., Waddle J.A., Schumacher J.M., Lin R. J. Cell Biol. 157:219-229(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Cell cycle- and swelling-induced activation of a Caenorhabditis elegans ClC channel is mediated by CeGLC-7alpha/beta phosphatases." Rutledge E., Denton J., Strange K. J. Cell Biol. 158:435-444(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis." Sasagawa Y., Urano T., Kohara Y., Takahashi H., Higashitani A. Genes Cells 8:857-872(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73974 Genomic DNA. Translation: CAA98273.1.
PIR	T21553.
RefSeq	NP_001256249.1. NM_001269320.1. NP_001256250.1. NM_001269321.1.
3D structure databases
HSSP	HSSP built from PDB template 1S70 based on UniProtKB P62140.
ProteinModelPortal	Q27497.
SMR	Q27497. Positions 4-309.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-55373N.
IntAct	Q27497. 3 interactions.
STRING	6239.F29F11.6.
Proteomic databases
PaxDb	Q27497.
PRIDE	Q27497.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F29F11.6a; F29F11.6a; F29F11.6. F29F11.6b; F29F11.6b; F29F11.6.
GeneID	179486.
KEGG	cel:CELE_F29F11.6.
UCSC	F29F11.6. c. elegans.
Organism-specific databases
CTD	179486.
WormBase	F29F11.6; CE20735; WBGene00001747; gsp-1.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00530000062911.
HOGENOM	HOG000172697.
InParanoid	Q27497.
KO	K06269.
OMA	PDLQGME.
Gene expression databases
ArrayExpress	Q27497.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	905606.

Entry information

Entry name

GLC7A_CAEEL

Accession

Primary (citable) accession number: Q27497

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 1, 1999
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project