Reviewed, UniProtKB/Swiss-Prot Q27483 (K6PF_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable 6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

ORF Names:

C50F4.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	Probable 6-phosphofructokinase		PRO_0000112029
Regions
	Nucleotide binding	30 – 34	5	ATP By similarity
	Nucleotide binding	186 – 190	5	ATP By similarity
	Nucleotide binding	203 – 219	17	ATP By similarity
Sites
	Active site	159	1	Proton acceptor By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	285	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	294	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27483-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 26A89B801D286534	FASTA	756	83,301
	10 20 30 40 50 60 MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY NGLINGNIEL 70 80 90 100 110 120 AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD KRIFHLIVIG GDGSLMGAQK 130 140 150 160 170 180 LKEEWGRFGE ELFAEGKITE EVANEGRELH LAGIVGSIDN DCIESDKSIG SDTALHRICE 190 200 210 220 230 240 AIDGLVMTAQ SHQRVFVVEV MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL 250 260 270 280 290 300 GSVRKMGKRQ NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF 310 320 330 340 350 360 LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE LSRVIRQTET 370 380 390 400 410 420 ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP SRTKTFAVVH IGSPCAGMNA 430 440 450 460 470 480 ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD VKLLTWANVQ GWAQFGGSML GTKRQLPSEM 490 500 510 520 530 540 DLIAEGLNSN NVDGLVIIGG FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM 550 560 570 580 590 600 SLGVDTALNE ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV 610 620 630 640 650 660 ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV MKNKYGVRIT 670 680 690 700 710 720 NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD CCVIGLRGSS LRYVPVQGLG 730 740 750 KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ EAVLSS

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70750 Genomic DNA. Translation: CAA94737.1.
PIR	T20109.
RefSeq	NP_505457.1.
UniGene	Cel.5512
3D structure databases
SMR	Q27483. Positions 404-737.
ModBase	Search...
Protein-protein interaction databases
STRING	Q27483.
Genome annotation databases
Ensembl	C50F4.2; C50F4.2; C50F4.2; Caenorhabditis elegans. [Genome view]
GeneID	179335.
KEGG	cel:C50F4.2.
NMPDR	fig|6239.3.peg.19126.
UCSC	C50F4.2. c. elegans.
Organism-specific databases
CTD	179335.
WormBase	WBGene00008230. C50F4.2.
WormPep	C50F4.2. CE05467. [WorfDB]
Phylogenomic databases
eggNOG	meNOG05673.
HOGENOM	HBG324640.
InParanoid	Q27483.
OMA	HRICEAI.
PhylomeDB	Q27483.
Enzyme and pathway databases
BRENDA	2.7.1.11. 672.
Gene expression databases
ArrayExpress	Q27483.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	904966.

Entry information

Entry name

K6PF_CAEEL

Accession

Primary (citable) accession number: Q27483

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project