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Q27483

- PFKA2_CAEEL

UniProt

Q27483 - PFKA2_CAEEL

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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

C50F4.2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201ATP; via amide nitrogenBy similarity
Metal bindingi112 – 1121Magnesium; catalyticBy similarity
Active sitei159 – 1591Proton acceptorBy similarity
Binding sitei194 – 1941Substrate; shared with dimeric partnerBy similarity
Binding sitei257 – 2571SubstrateBy similarity
Binding sitei285 – 2851Substrate; shared with dimeric partnerBy similarity
Binding sitei474 – 4741Allosteric activator fructose 2,6-bisphosphateBy similarity
Binding sitei632 – 6321Allosteric activator fructose 2,6-bisphosphateBy similarity
Binding sitei658 – 6581Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 822ATPBy similarity
Nucleotide bindingi111 – 1144ATPBy similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11)
Short name:
ATP-PFK 2
Short name:
Phosphofructokinase 2
Alternative name(s):
Phosphohexokinase 2
Gene namesi
ORF Names:C50F4.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiC50F4.2; CE05467; WBGene00008230.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 756756ATP-dependent 6-phosphofructokinase 2PRO_0000112029Add
BLAST

Proteomic databases

PaxDbiQ27483.
PRIDEiQ27483.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi6239.C50F4.2.

Structurei

3D structure databases

ProteinModelPortaliQ27483.
SMRiQ27483. Positions 8-747.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 393393N-terminal catalytic PFK domain 1Add
BLAST
Regioni157 – 1593Substrate bindingBy similarity
Regioni201 – 2033Substrate bindingBy similarity
Regioni291 – 2944Substrate bindingBy similarity
Regioni394 – 40411Interdomain linkerAdd
BLAST
Regioni405 – 756352C-terminal regulatory PFK domain 2Add
BLAST
Regioni530 – 5345Allosteric activator fructose 2,6-bisphosphate bindingBy similarity
Regioni575 – 5773Allosteric activator fructose 2,6-bisphosphate bindingBy similarity
Regioni664 – 6674Allosteric activator fructose 2,6-bisphosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
InParanoidiQ27483.
KOiK00850.
OMAiATISNNC.
OrthoDBiEOG7ZSHV5.
PhylomeDBiQ27483.

Family and domain databases

InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27483-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY
60 70 80 90 100
NGLINGNIEL AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD
110 120 130 140 150
KRIFHLIVIG GDGSLMGAQK LKEEWGRFGE ELFAEGKITE EVANEGRELH
160 170 180 190 200
LAGIVGSIDN DCIESDKSIG SDTALHRICE AIDGLVMTAQ SHQRVFVVEV
210 220 230 240 250
MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL GSVRKMGKRQ
260 270 280 290 300
NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF
310 320 330 340 350
LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE
360 370 380 390 400
LSRVIRQTET ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP
410 420 430 440 450
SRTKTFAVVH IGSPCAGMNA ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD
460 470 480 490 500
VKLLTWANVQ GWAQFGGSML GTKRQLPSEM DLIAEGLNSN NVDGLVIIGG
510 520 530 540 550
FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM SLGVDTALNE
560 570 580 590 600
ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV
610 620 630 640 650
ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV
660 670 680 690 700
MKNKYGVRIT NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD
710 720 730 740 750
CCVIGLRGSS LRYVPVQGLG KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ

EAVLSS
Length:756
Mass (Da):83,301
Last modified:November 1, 1996 - v1
Checksum:i26A89B801D286534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70750 Genomic DNA. Translation: CAA94737.1.
PIRiT20109.
RefSeqiNP_505457.1. NM_073056.3.
UniGeneiCel.5512.

Genome annotation databases

EnsemblMetazoaiC50F4.2; C50F4.2; WBGene00008230.
GeneIDi179335.
KEGGicel:CELE_C50F4.2.
UCSCiC50F4.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70750 Genomic DNA. Translation: CAA94737.1 .
PIRi T20109.
RefSeqi NP_505457.1. NM_073056.3.
UniGenei Cel.5512.

3D structure databases

ProteinModelPortali Q27483.
SMRi Q27483. Positions 8-747.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.C50F4.2.

Proteomic databases

PaxDbi Q27483.
PRIDEi Q27483.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C50F4.2 ; C50F4.2 ; WBGene00008230 .
GeneIDi 179335.
KEGGi cel:CELE_C50F4.2.
UCSCi C50F4.2. c. elegans.

Organism-specific databases

CTDi 179335.
WormBasei C50F4.2 ; CE05467 ; WBGene00008230 .

Phylogenomic databases

eggNOGi COG0205.
GeneTreei ENSGT00390000013209.
HOGENOMi HOG000200154.
InParanoidi Q27483.
KOi K00850.
OMAi ATISNNC.
OrthoDBi EOG7ZSHV5.
PhylomeDBi Q27483.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Miscellaneous databases

NextBioi 904966.

Family and domain databases

InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiPFKA2_CAEEL
AccessioniPrimary (citable) accession number: Q27483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3