UniProtKB - Q27483 (PFKA2_CAEEL)
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Protein
ATP-dependent 6-phosphofructokinase 2
Gene
pfk-1.2
Organism
Caenorhabditis elegans
Status
Functioni
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity
Catalytic activityi
ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity
Cofactori
Mg2+By similarity
Enzyme regulationi
Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity
: glycolysis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarityProteins known to be involved in the 4 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Glucose-6-phosphate isomerase (gpi-1), Glucose-6-phosphate isomerase (gpi-1)
- ATP-dependent 6-phosphofructokinase 1 (pfk-1.1), ATP-dependent 6-phosphofructokinase 2 (pfk-1.2)
- Fructose-bisphosphate aldolase (aldo-2), Fructose-bisphosphate aldolase 2 (aldo-2), Fructose-bisphosphate aldolase 1 (aldo-1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 20 | ATP; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 112 | Magnesium; catalyticBy similarity | 1 | |
Active sitei | 159 | Proton acceptorBy similarity | 1 | |
Binding sitei | 194 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 257 | SubstrateBy similarity | 1 | |
Binding sitei | 285 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 474 | Allosteric activator fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 632 | Allosteric activator fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 658 | Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 81 – 82 | ATPBy similarity | 2 | |
Nucleotide bindingi | 111 – 114 | ATPBy similarity | 4 |
GO - Molecular functioni
- 6-phosphofructokinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- fructose 6-phosphate metabolic process Source: InterPro
Keywordsi
Molecular function | Allosteric enzyme, Kinase, Transferase |
Biological process | Glycolysis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109; UER00182. |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11By similarity)Short name: ATP-PFK 2 Short name: Phosphofructokinase 2 Alternative name(s): Phosphohexokinase 2 |
Gene namesi | Name:pfk-1.2Imported ORF Names:C50F4.2Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C50F4.2; CE05467; WBGene00008230; pfk-1.2. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000112029 | 1 – 756 | ATP-dependent 6-phosphofructokinase 2Add BLAST | 756 |
Proteomic databases
EPDi | Q27483. |
PaxDbi | Q27483. |
PeptideAtlasi | Q27483. |
Interactioni
Subunit structurei
Homotetramer.By similarity
Protein-protein interaction databases
STRINGi | 6239.C50F4.2. |
Structurei
3D structure databases
ProteinModelPortali | Q27483. |
SMRi | Q27483. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 393 | N-terminal catalytic PFK domain 1By similarityAdd BLAST | 393 | |
Regioni | 157 – 159 | Substrate bindingBy similarity | 3 | |
Regioni | 201 – 203 | Substrate bindingBy similarity | 3 | |
Regioni | 291 – 294 | Substrate bindingBy similarity | 4 | |
Regioni | 394 – 404 | Interdomain linkerBy similarityAdd BLAST | 11 | |
Regioni | 405 – 756 | C-terminal regulatory PFK domain 2By similarityAdd BLAST | 352 | |
Regioni | 530 – 534 | Allosteric activator fructose 2,6-bisphosphate bindingBy similarity | 5 | |
Regioni | 575 – 577 | Allosteric activator fructose 2,6-bisphosphate bindingBy similarity | 3 | |
Regioni | 664 – 667 | Allosteric activator fructose 2,6-bisphosphate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.Curated
Phylogenomic databases
eggNOGi | KOG2440. Eukaryota. COG0205. LUCA. |
GeneTreei | ENSGT00390000013209. |
HOGENOMi | HOG000200154. |
InParanoidi | Q27483. |
KOi | K00850. |
OMAi | ATISNNC. |
OrthoDBi | EOG091G02KN. |
PhylomeDBi | Q27483. |
Family and domain databases
InterProi | View protein in InterPro IPR009161. 6-Pfructokinase_euk. IPR022953. ATP_PFK. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. IPR035966. PKF_sf. |
Pfami | View protein in Pfam PF00365. PFK. 2 hits. |
PIRSFi | PIRSF000533. ATP_PFK_euk. 1 hit. |
PRINTSi | PR00476. PHFRCTKINASE. |
SUPFAMi | SSF53784. SSF53784. 2 hits. |
TIGRFAMsi | TIGR02478. 6PF1K_euk. 1 hit. |
PROSITEi | View protein in PROSITE PS00433. PHOSPHOFRUCTOKINASE. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q27483-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY
60 70 80 90 100
NGLINGNIEL AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD
110 120 130 140 150
KRIFHLIVIG GDGSLMGAQK LKEEWGRFGE ELFAEGKITE EVANEGRELH
160 170 180 190 200
LAGIVGSIDN DCIESDKSIG SDTALHRICE AIDGLVMTAQ SHQRVFVVEV
210 220 230 240 250
MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL GSVRKMGKRQ
260 270 280 290 300
NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF
310 320 330 340 350
LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE
360 370 380 390 400
LSRVIRQTET ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP
410 420 430 440 450
SRTKTFAVVH IGSPCAGMNA ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD
460 470 480 490 500
VKLLTWANVQ GWAQFGGSML GTKRQLPSEM DLIAEGLNSN NVDGLVIIGG
510 520 530 540 550
FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM SLGVDTALNE
560 570 580 590 600
ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV
610 620 630 640 650
ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV
660 670 680 690 700
MKNKYGVRIT NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD
710 720 730 740 750
CCVIGLRGSS LRYVPVQGLG KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ
EAVLSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z70750 Genomic DNA. Translation: CAA94737.1. |
PIRi | T20109. |
RefSeqi | NP_505457.1. NM_073056.3. |
UniGenei | Cel.5512. |
Genome annotation databases
EnsemblMetazoai | C50F4.2; C50F4.2; WBGene00008230. |
GeneIDi | 179335. |
KEGGi | cel:CELE_C50F4.2. |
UCSCi | C50F4.2. c. elegans. |
Similar proteinsi
Entry informationi
Entry namei | PFKA2_CAEEL | |
Accessioni | Q27483Primary (citable) accession number: Q27483 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 28, 2018 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |