pfk-1.2

Functionⁱ

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityⁱ

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity

Cofactorⁱ

Mg²⁺By similarity

Enzyme regulationⁱ

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity

Pathwayⁱ: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

no protein annotated in this organism
Glucose-6-phosphate isomerase (gpi-1), Glucose-6-phosphate isomerase (gpi-1)
ATP-dependent 6-phosphofructokinase 1 (pfk-1.1), ATP-dependent 6-phosphofructokinase 2 (pfk-1.2)
Fructose-bisphosphate aldolase (aldo-2), Fructose-bisphosphate aldolase 2 (aldo-2), Fructose-bisphosphate aldolase 1 (aldo-1)

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	20	ATP; via amide nitrogenBy similarity	1
Metal bindingⁱ	112	Magnesium; catalyticBy similarity	1
Active siteⁱ	159	Proton acceptorBy similarity	1
Binding siteⁱ	194	Substrate; shared with dimeric partnerBy similarity	1
Binding siteⁱ	257	SubstrateBy similarity	1
Binding siteⁱ	285	Substrate; shared with dimeric partnerBy similarity	1
Binding siteⁱ	474	Allosteric activator fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	632	Allosteric activator fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	658	Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	81 – 82	ATPBy similarity		2
Nucleotide bindingⁱ	111 – 114	ATPBy similarity		4

GO - Molecular functionⁱ

6-phosphofructokinase activity Source: UniProtKB-EC
ATP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

fructose 6-phosphate metabolic process Source: InterPro

Complete GO annotation...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Glycolysis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-CEL-6798695. Neutrophil degranulation. R-CEL-70171. Glycolysis.
UniPathwayⁱ	UPA00109; UER00182.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11By similarity) Short name: ATP-PFK 2 Short name: Phosphofructokinase 2 Alternative name(s): Phosphohexokinase 2
Gene namesⁱ	Name:pfk-1.2Imported ORF Names:C50F4.2Imported
Organismⁱ	Caenorhabditis elegans
Taxonomic identifierⁱ	6239 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis
Proteomesⁱ	UP000001940 Componentⁱ: Chromosome V

Organism-specific databases

WormBase More...WormBaseⁱ	C50F4.2; CE05467; WBGene00008230; pfk-1.2.

WormBaseⁱ

C50F4.2; CE05467; WBGene00008230; pfk-1.2.

Subcellular locationⁱ

Cytoplasm
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:P16861 (PFKA1_YEAST)

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000112029	1 – 756	ATP-dependent 6-phosphofructokinase 2Add BLAST		756

Proteomic databases

EPDⁱ	Q27483.
PaxDbⁱ	Q27483.
PeptideAtlas More...PeptideAtlasⁱ	Q27483.

Expressionⁱ

Gene expression databases

Bgeeⁱ	WBGene00008230.

Bgeeⁱ

WBGene00008230.

Interactionⁱ

Subunit structureⁱ

Homotetramer.By similarity

Protein-protein interaction databases

STRINGⁱ	6239.C50F4.2.

STRINGⁱ

6239.C50F4.2.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q27483.
SMRⁱ	Q27483.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 393	N-terminal catalytic PFK domain 1By similarityAdd BLAST	393
Regionⁱ	157 – 159	Substrate bindingBy similarity	3
Regionⁱ	201 – 203	Substrate bindingBy similarity	3
Regionⁱ	291 – 294	Substrate bindingBy similarity	4
Regionⁱ	394 – 404	Interdomain linkerBy similarityAdd BLAST	11
Regionⁱ	405 – 756	C-terminal regulatory PFK domain 2By similarityAdd BLAST	352
Regionⁱ	530 – 534	Allosteric activator fructose 2,6-bisphosphate bindingBy similarity	5
Regionⁱ	575 – 577	Allosteric activator fructose 2,6-bisphosphate bindingBy similarity	3
Regionⁱ	664 – 667	Allosteric activator fructose 2,6-bisphosphate bindingBy similarity	4

Sequence similaritiesⁱ

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG2440. Eukaryota. COG0205. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000013209.
HOGENOMⁱ	HOG000200154.
InParanoidⁱ	Q27483.
KEGG Orthology (KO) More...KOⁱ	K00850.
OMAⁱ	ATISNNC.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02KN.
PhylomeDBⁱ	Q27483.

Family and domain databases

InterProⁱ	View protein in InterPro IPR009161. 6-Pfructokinase_euk. IPR022953. ATP_PFK. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00365. PFK. 2 hits.
PIRSFⁱ	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSⁱ	PR00476. PHFRCTKINASE.
SUPFAMⁱ	SSF53784. SSF53784. 2 hits.
TIGRFAMsⁱ	TIGR02478. 6PF1K_euk. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00433. PHOSPHOFRUCTOKINASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q27483-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY 
        60         70         80         90        100
NGLINGNIEL AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD 
       110        120        130        140        150
KRIFHLIVIG GDGSLMGAQK LKEEWGRFGE ELFAEGKITE EVANEGRELH 
       160        170        180        190        200
LAGIVGSIDN DCIESDKSIG SDTALHRICE AIDGLVMTAQ SHQRVFVVEV 
       210        220        230        240        250
MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL GSVRKMGKRQ 
       260        270        280        290        300
NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF 
       310        320        330        340        350
LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE 
       360        370        380        390        400
LSRVIRQTET ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP 
       410        420        430        440        450
SRTKTFAVVH IGSPCAGMNA ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD 
       460        470        480        490        500
VKLLTWANVQ GWAQFGGSML GTKRQLPSEM DLIAEGLNSN NVDGLVIIGG 
       510        520        530        540        550
FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM SLGVDTALNE 
       560        570        580        590        600
ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV 
       610        620        630        640        650
ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV 
       660        670        680        690        700
MKNKYGVRIT NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD 
       710        720        730        740        750
CCVIGLRGSS LRYVPVQGLG KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ 

EAVLSS

Length:756

Mass (Da):83,301

Last modified:November 1, 1996 - v1

Checksum:ⁱ26A89B801D286534

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z70750 Genomic DNA. Translation: CAA94737.1.
PIRⁱ	T20109.
NCBI Reference Sequences More...RefSeqⁱ	NP_505457.1. NM_073056.3.
UniGeneⁱ	Cel.5512.

Genome annotation databases

EnsemblMetazoaⁱ	C50F4.2; C50F4.2; WBGene00008230.
GeneIDⁱ	179335.
KEGGⁱ	cel:CELE_C50F4.2.
UCSC genome browser More...UCSCⁱ	C50F4.2. c. elegans.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z70750 Genomic DNA. Translation: CAA94737.1.
PIRⁱ	T20109.
NCBI Reference Sequences More...RefSeqⁱ	NP_505457.1. NM_073056.3.
UniGeneⁱ	Cel.5512.

3D structure databases

ProteinModelPortalⁱ	Q27483.
SMRⁱ	Q27483.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	6239.C50F4.2.

STRINGⁱ

6239.C50F4.2.

Proteomic databases

EPDⁱ	Q27483.
PaxDbⁱ	Q27483.
PeptideAtlas More...PeptideAtlasⁱ	Q27483.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblMetazoaⁱ	C50F4.2; C50F4.2; WBGene00008230.
GeneIDⁱ	179335.
KEGGⁱ	cel:CELE_C50F4.2.
UCSC genome browser More...UCSCⁱ	C50F4.2. c. elegans.

Organism-specific databases

CTDⁱ	179335.
WormBase More...WormBaseⁱ	C50F4.2; CE05467; WBGene00008230; pfk-1.2.

Phylogenomic databases

eggNOGⁱ	KOG2440. Eukaryota. COG0205. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000013209.
HOGENOMⁱ	HOG000200154.
InParanoidⁱ	Q27483.
KEGG Orthology (KO) More...KOⁱ	K00850.
OMAⁱ	ATISNNC.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02KN.
PhylomeDBⁱ	Q27483.

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00182.
Reactomeⁱ	R-CEL-6798695. Neutrophil degranulation. R-CEL-70171. Glycolysis.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q27483.

PROⁱ

PR:Q27483.

Gene expression databases

Bgeeⁱ	WBGene00008230.

Bgeeⁱ

WBGene00008230.

Family and domain databases

InterProⁱ	View protein in InterPro IPR009161. 6-Pfructokinase_euk. IPR022953. ATP_PFK. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00365. PFK. 2 hits.
PIRSFⁱ	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSⁱ	PR00476. PHFRCTKINASE.
SUPFAMⁱ	SSF53784. SSF53784. 2 hits.
TIGRFAMsⁱ	TIGR02478. 6PF1K_euk. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PFKA2_CAEEL
Accessionⁱ	Q27483Primary (citable) accession number: Q27483
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	November 1, 1996
	Last modified:	March 15, 2017
	This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Caenorhabditis annotation project

Miscellaneousⁱ

Keywords - Technical termⁱ

Allosteric enzyme, Complete proteome, Reference proteome

Documents

Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q27483 (PFKA2_CAEEL)

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ATP-dependent 6-phosphofructokinase 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Enzyme regulationⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ