Q27483 (PFKA2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent 6-phosphofructokinase 2
Short name=ATP-PFK 2
Short name=Phosphofructokinase 2
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase 2

Gene names

ORF Names:

C50F4.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.
Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolytic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: UniProtKB-EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	ATP-dependent 6-phosphofructokinase 2		PRO_0000112029
Regions
	Nucleotide binding	81 – 82	2	ATP By similarity
	Nucleotide binding	111 – 114	4	ATP By similarity
	Region	1 – 393	393	N-terminal catalytic PFK domain 1
	Region	157 – 159	3	Substrate binding By similarity
	Region	201 – 203	3	Substrate binding By similarity
	Region	291 – 294	4	Substrate binding By similarity
	Region	394 – 404	11	Interdomain linker
	Region	405 – 756	352	C-terminal regulatory PFK domain 2
	Region	530 – 534	5	Allosteric activator fructose 2,6-bisphosphate binding By similarity
	Region	575 – 577	3	Allosteric activator fructose 2,6-bisphosphate binding By similarity
	Region	664 – 667	4	Allosteric activator fructose 2,6-bisphosphate binding By similarity
Sites
	Active site	159	1	Proton acceptor By similarity
	Metal binding	112	1	Magnesium; catalytic By similarity
	Binding site	20	1	ATP; via amide nitrogen By similarity
	Binding site	194	1	Substrate; shared with dimeric partner By similarity
	Binding site	257	1	Substrate By similarity
	Binding site	285	1	Substrate; shared with dimeric partner By similarity
	Binding site	474	1	Allosteric activator fructose 2,6-bisphosphate By similarity
	Binding site	632	1	Allosteric activator fructose 2,6-bisphosphate By similarity
	Binding site	658	1	Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27483 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 26A89B801D286534	FASTA	756	83,301
	10 20 30 40 50 60 MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY NGLINGNIEL 70 80 90 100 110 120 AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD KRIFHLIVIG GDGSLMGAQK 130 140 150 160 170 180 LKEEWGRFGE ELFAEGKITE EVANEGRELH LAGIVGSIDN DCIESDKSIG SDTALHRICE 190 200 210 220 230 240 AIDGLVMTAQ SHQRVFVVEV MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL 250 260 270 280 290 300 GSVRKMGKRQ NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF 310 320 330 340 350 360 LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE LSRVIRQTET 370 380 390 400 410 420 ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP SRTKTFAVVH IGSPCAGMNA 430 440 450 460 470 480 ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD VKLLTWANVQ GWAQFGGSML GTKRQLPSEM 490 500 510 520 530 540 DLIAEGLNSN NVDGLVIIGG FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM 550 560 570 580 590 600 SLGVDTALNE ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV 610 620 630 640 650 660 ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV MKNKYGVRIT 670 680 690 700 710 720 NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD CCVIGLRGSS LRYVPVQGLG 730 740 750 KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ EAVLSS

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70750 Genomic DNA. Translation: CAA94737.1.
PIR	T20109.
RefSeq	NP_505457.1. NM_073056.3.
UniGene	Cel.5512.
3D structure databases
ProteinModelPortal	Q27483.
SMR	Q27483. Positions 8-747.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	6239.C50F4.2.
Proteomic databases
PaxDb	Q27483.
PRIDE	Q27483.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C50F4.2; C50F4.2; WBGene00008230.
GeneID	179335.
KEGG	cel:CELE_C50F4.2.
UCSC	C50F4.2. c. elegans.
Organism-specific databases
CTD	179335.
WormBase	C50F4.2; CE05467; WBGene00008230.
Phylogenomic databases
eggNOG	COG0205.
GeneTree	ENSGT00390000013209.
HOGENOM	HOG000200154.
InParanoid	Q27483.
KO	K00850.
OMA	ATISNNC.
OrthoDB	EOG7ZSHV5.
PhylomeDB	Q27483.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. SSF53784. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	904966.

Entry information

Entry name

PFKA2_CAEEL

Accession

Primary (citable) accession number: Q27483

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project