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Q27483 (PFKA2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 2

Short name=ATP-PFK 2
Short name=Phosphofructokinase 2
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 2
Gene names
ORF Names:C50F4.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length756 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactor

Magnesium By similarity.

Enzyme regulation

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 756756ATP-dependent 6-phosphofructokinase 2
PRO_0000112029

Regions

Nucleotide binding81 – 822ATP By similarity
Nucleotide binding111 – 1144ATP By similarity
Region1 – 393393N-terminal catalytic PFK domain 1
Region157 – 1593Substrate binding By similarity
Region201 – 2033Substrate binding By similarity
Region291 – 2944Substrate binding By similarity
Region394 – 40411Interdomain linker
Region405 – 756352C-terminal regulatory PFK domain 2
Region530 – 5345Allosteric activator fructose 2,6-bisphosphate binding By similarity
Region575 – 5773Allosteric activator fructose 2,6-bisphosphate binding By similarity
Region664 – 6674Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sites

Active site1591Proton acceptor By similarity
Metal binding1121Magnesium; catalytic By similarity
Binding site201ATP; via amide nitrogen By similarity
Binding site1941Substrate; shared with dimeric partner By similarity
Binding site2571Substrate By similarity
Binding site2851Substrate; shared with dimeric partner By similarity
Binding site4741Allosteric activator fructose 2,6-bisphosphate By similarity
Binding site6321Allosteric activator fructose 2,6-bisphosphate By similarity
Binding site6581Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27483 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 26A89B801D286534

FASTA75683,301
        10         20         30         40         50         60 
MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY NGLINGNIEL 

        70         80         90        100        110        120 
AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD KRIFHLIVIG GDGSLMGAQK 

       130        140        150        160        170        180 
LKEEWGRFGE ELFAEGKITE EVANEGRELH LAGIVGSIDN DCIESDKSIG SDTALHRICE 

       190        200        210        220        230        240 
AIDGLVMTAQ SHQRVFVVEV MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL 

       250        260        270        280        290        300 
GSVRKMGKRQ NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF 

       310        320        330        340        350        360 
LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE LSRVIRQTET 

       370        380        390        400        410        420 
ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP SRTKTFAVVH IGSPCAGMNA 

       430        440        450        460        470        480 
ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD VKLLTWANVQ GWAQFGGSML GTKRQLPSEM 

       490        500        510        520        530        540 
DLIAEGLNSN NVDGLVIIGG FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM 

       550        560        570        580        590        600 
SLGVDTALNE ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV 

       610        620        630        640        650        660 
ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV MKNKYGVRIT 

       670        680        690        700        710        720 
NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD CCVIGLRGSS LRYVPVQGLG 

       730        740        750 
KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ EAVLSS 

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References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z70750 Genomic DNA. Translation: CAA94737.1.
PIRT20109.
RefSeqNP_505457.1. NM_073056.3.
UniGeneCel.5512.

3D structure databases

ProteinModelPortalQ27483.
SMRQ27483. Positions 8-747.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.C50F4.2.

Proteomic databases

PaxDbQ27483.
PRIDEQ27483.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC50F4.2; C50F4.2; WBGene00008230.
GeneID179335.
KEGGcel:CELE_C50F4.2.
UCSCC50F4.2. c. elegans.

Organism-specific databases

CTD179335.
WormBaseC50F4.2; CE05467; WBGene00008230.

Phylogenomic databases

eggNOGCOG0205.
GeneTreeENSGT00390000013209.
HOGENOMHOG000200154.
InParanoidQ27483.
KOK00850.
OMAATISNNC.
OrthoDBEOG7ZSHV5.
PhylomeDBQ27483.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

InterProIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 2 hits.
[Graphical view]
PIRSFPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 2 hits.
TIGRFAMsTIGR02478. 6PF1K_euk. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio904966.

Entry information

Entry namePFKA2_CAEEL
AccessionPrimary (citable) accession number: Q27483
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase