Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q27483

- PFKA2_CAEEL

UniProt

Q27483 - PFKA2_CAEEL

Protein

ATP-dependent 6-phosphofructokinase 2

Gene

C50F4.2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201ATP; via amide nitrogenBy similarity
    Metal bindingi112 – 1121Magnesium; catalyticBy similarity
    Active sitei159 – 1591Proton acceptorBy similarity
    Binding sitei194 – 1941Substrate; shared with dimeric partnerBy similarity
    Binding sitei257 – 2571SubstrateBy similarity
    Binding sitei285 – 2851Substrate; shared with dimeric partnerBy similarity
    Binding sitei474 – 4741Allosteric activator fructose 2,6-bisphosphateBy similarity
    Binding sitei632 – 6321Allosteric activator fructose 2,6-bisphosphateBy similarity
    Binding sitei658 – 6581Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 822ATPBy similarity
    Nucleotide bindingi111 – 1144ATPBy similarity

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11)
    Short name:
    ATP-PFK 2
    Short name:
    Phosphofructokinase 2
    Alternative name(s):
    Phosphohexokinase 2
    Gene namesi
    ORF Names:C50F4.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiC50F4.2; CE05467; WBGene00008230.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 756756ATP-dependent 6-phosphofructokinase 2PRO_0000112029Add
    BLAST

    Proteomic databases

    PaxDbiQ27483.
    PRIDEiQ27483.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi6239.C50F4.2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27483.
    SMRiQ27483. Positions 8-747.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 393393N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni157 – 1593Substrate bindingBy similarity
    Regioni201 – 2033Substrate bindingBy similarity
    Regioni291 – 2944Substrate bindingBy similarity
    Regioni394 – 40411Interdomain linkerAdd
    BLAST
    Regioni405 – 756352C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni530 – 5345Allosteric activator fructose 2,6-bisphosphate bindingBy similarity
    Regioni575 – 5773Allosteric activator fructose 2,6-bisphosphate bindingBy similarity
    Regioni664 – 6674Allosteric activator fructose 2,6-bisphosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0205.
    GeneTreeiENSGT00390000013209.
    HOGENOMiHOG000200154.
    InParanoidiQ27483.
    KOiK00850.
    OMAiATISNNC.
    OrthoDBiEOG7ZSHV5.
    PhylomeDBiQ27483.

    Family and domain databases

    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27483-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY    50
    NGLINGNIEL AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD 100
    KRIFHLIVIG GDGSLMGAQK LKEEWGRFGE ELFAEGKITE EVANEGRELH 150
    LAGIVGSIDN DCIESDKSIG SDTALHRICE AIDGLVMTAQ SHQRVFVVEV 200
    MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL GSVRKMGKRQ 250
    NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF 300
    LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE 350
    LSRVIRQTET ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP 400
    SRTKTFAVVH IGSPCAGMNA ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD 450
    VKLLTWANVQ GWAQFGGSML GTKRQLPSEM DLIAEGLNSN NVDGLVIIGG 500
    FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM SLGVDTALNE 550
    ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV 600
    ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV 650
    MKNKYGVRIT NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD 700
    CCVIGLRGSS LRYVPVQGLG KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ 750
    EAVLSS 756
    Length:756
    Mass (Da):83,301
    Last modified:November 1, 1996 - v1
    Checksum:i26A89B801D286534
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70750 Genomic DNA. Translation: CAA94737.1.
    PIRiT20109.
    RefSeqiNP_505457.1. NM_073056.3.
    UniGeneiCel.5512.

    Genome annotation databases

    EnsemblMetazoaiC50F4.2; C50F4.2; WBGene00008230.
    GeneIDi179335.
    KEGGicel:CELE_C50F4.2.
    UCSCiC50F4.2. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70750 Genomic DNA. Translation: CAA94737.1 .
    PIRi T20109.
    RefSeqi NP_505457.1. NM_073056.3.
    UniGenei Cel.5512.

    3D structure databases

    ProteinModelPortali Q27483.
    SMRi Q27483. Positions 8-747.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.C50F4.2.

    Proteomic databases

    PaxDbi Q27483.
    PRIDEi Q27483.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C50F4.2 ; C50F4.2 ; WBGene00008230 .
    GeneIDi 179335.
    KEGGi cel:CELE_C50F4.2.
    UCSCi C50F4.2. c. elegans.

    Organism-specific databases

    CTDi 179335.
    WormBasei C50F4.2 ; CE05467 ; WBGene00008230 .

    Phylogenomic databases

    eggNOGi COG0205.
    GeneTreei ENSGT00390000013209.
    HOGENOMi HOG000200154.
    InParanoidi Q27483.
    KOi K00850.
    OMAi ATISNNC.
    OrthoDBi EOG7ZSHV5.
    PhylomeDBi Q27483.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Miscellaneous databases

    NextBioi 904966.

    Family and domain databases

    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiPFKA2_CAEEL
    AccessioniPrimary (citable) accession number: Q27483
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3