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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

C50F4.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.By similarity

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (gpi-1), Glucose-6-phosphate isomerase (gpi-1)
  3. ATP-dependent 6-phosphofructokinase 1 (pfk-1), ATP-dependent 6-phosphofructokinase 2 (C50F4.2)
  4. Fructose-bisphosphate aldolase (aldo-2), Fructose-bisphosphate aldolase 2 (aldo-2), Fructose-bisphosphate aldolase 1 (aldo-1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20ATP; via amide nitrogenBy similarity1
Metal bindingi112Magnesium; catalyticBy similarity1
Active sitei159Proton acceptorBy similarity1
Binding sitei194Substrate; shared with dimeric partnerBy similarity1
Binding sitei257SubstrateBy similarity1
Binding sitei285Substrate; shared with dimeric partnerBy similarity1
Binding sitei474Allosteric activator fructose 2,6-bisphosphateBy similarity1
Binding sitei632Allosteric activator fructose 2,6-bisphosphateBy similarity1
Binding sitei658Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 82ATPBy similarity2
Nucleotide bindingi111 – 114ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-6798695. Neutrophil degranulation.
R-CEL-70171. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11)
Short name:
ATP-PFK 2
Short name:
Phosphofructokinase 2
Alternative name(s):
Phosphohexokinase 2
Gene namesi
ORF Names:C50F4.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC50F4.2; CE05467; WBGene00008230.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120291 – 756ATP-dependent 6-phosphofructokinase 2Add BLAST756

Proteomic databases

EPDiQ27483.
PaxDbiQ27483.
PeptideAtlasiQ27483.

Expressioni

Gene expression databases

BgeeiWBGene00008230.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi6239.C50F4.2.

Structurei

3D structure databases

ProteinModelPortaliQ27483.
SMRiQ27483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 393N-terminal catalytic PFK domain 1By similarityAdd BLAST393
Regioni157 – 159Substrate bindingBy similarity3
Regioni201 – 203Substrate bindingBy similarity3
Regioni291 – 294Substrate bindingBy similarity4
Regioni394 – 404Interdomain linkerBy similarityAdd BLAST11
Regioni405 – 756C-terminal regulatory PFK domain 2By similarityAdd BLAST352
Regioni530 – 534Allosteric activator fructose 2,6-bisphosphate bindingBy similarity5
Regioni575 – 577Allosteric activator fructose 2,6-bisphosphate bindingBy similarity3
Regioni664 – 667Allosteric activator fructose 2,6-bisphosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
InParanoidiQ27483.
KOiK00850.
OMAiATISNNC.
OrthoDBiEOG091G02KN.
PhylomeDBiQ27483.

Family and domain databases

InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQKFKKGKD HGVGVLTSGG DSQGMNAAVR SVVRETIRQG HRCYLIREGY
60 70 80 90 100
NGLINGNIEL AKWAHVANVT HLGGSMIGTS RCDEFRTTDG RKKAAAIMFD
110 120 130 140 150
KRIFHLIVIG GDGSLMGAQK LKEEWGRFGE ELFAEGKITE EVANEGRELH
160 170 180 190 200
LAGIVGSIDN DCIESDKSIG SDTALHRICE AIDGLVMTAQ SHQRVFVVEV
210 220 230 240 250
MGRHCGYLAL TAAIAVEADY VFYPEIPPDE KWPEQLCHQL GSVRKMGKRQ
260 270 280 290 300
NVIILGEGVT NSKGQRIDVR QVKEEIETRL QLEVRIATLG HLQRGGAPSF
310 320 330 340 350
LDRLIGLRMG YEAVQEVLKG KEEKEGAVVT GQKTIAKVMC LRGHNIQRNE
360 370 380 390 400
LSRVIRQTET ANEEIMQRHS DLACRLRGFG FLDKQTYLNF VSIPLSTTMP
410 420 430 440 450
SRTKTFAVVH IGSPCAGMNA ATYSFTRMAN HSGIQVIGIK HGWDGLKNKD
460 470 480 490 500
VKLLTWANVQ GWAQFGGSML GTKRQLPSEM DLIAEGLNSN NVDGLVIIGG
510 520 530 540 550
FMAFESALIL QQNRSEYTCL SIPIVVIPAT ISNNCPGTCM SLGVDTALNE
560 570 580 590 600
ICRQVDNISQ NAIGSKNKVM IIETMGSRSG FLATMTALST GSQFALIRQV
610 620 630 640 650
ETNEKDLEKL AIETKERLDS GNLEKFLLIR SEGASDEIYS PDVKKIFDKV
660 670 680 690 700
MKNKYGVRIT NLGYSQLGGH PSCFDRQMGI RMGVRAFEGI VNPVKMGDRD
710 720 730 740 750
CCVIGLRGSS LRYVPVQGLG KKVCFEHGVP HNMWWLDLHP LVEAMTKKPQ

EAVLSS
Length:756
Mass (Da):83,301
Last modified:November 1, 1996 - v1
Checksum:i26A89B801D286534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70750 Genomic DNA. Translation: CAA94737.1.
PIRiT20109.
RefSeqiNP_505457.1. NM_073056.3.
UniGeneiCel.5512.

Genome annotation databases

EnsemblMetazoaiC50F4.2; C50F4.2; WBGene00008230.
GeneIDi179335.
KEGGicel:CELE_C50F4.2.
UCSCiC50F4.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70750 Genomic DNA. Translation: CAA94737.1.
PIRiT20109.
RefSeqiNP_505457.1. NM_073056.3.
UniGeneiCel.5512.

3D structure databases

ProteinModelPortaliQ27483.
SMRiQ27483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C50F4.2.

Proteomic databases

EPDiQ27483.
PaxDbiQ27483.
PeptideAtlasiQ27483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC50F4.2; C50F4.2; WBGene00008230.
GeneIDi179335.
KEGGicel:CELE_C50F4.2.
UCSCiC50F4.2. c. elegans.

Organism-specific databases

CTDi179335.
WormBaseiC50F4.2; CE05467; WBGene00008230.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
InParanoidiQ27483.
KOiK00850.
OMAiATISNNC.
OrthoDBiEOG091G02KN.
PhylomeDBiQ27483.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
ReactomeiR-CEL-6798695. Neutrophil degranulation.
R-CEL-70171. Glycolysis.

Miscellaneous databases

PROiQ27483.

Gene expression databases

BgeeiWBGene00008230.

Family and domain databases

InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA2_CAEEL
AccessioniPrimary (citable) accession number: Q27483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.