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Q27474 (DNLI1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase 1
EC=6.5.1.1
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene names
Name:lig-1
ORF Names:C29A12.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length773 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q27474-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: Q27474-2)

The sequence of this isoform differs from the canonical sequence as follows:
     208-210: VRG → RMP
     211-773: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 773773DNA ligase 1
PRO_0000059583

Regions

Region180 – 18910Interaction with target DNA By similarity
Region362 – 3643Interaction with target DNA By similarity
Compositional bias638 – 64811Poly-Glu
Compositional bias660 – 762103Lys-rich

Sites

Active site2891N6-AMP-lysine intermediate By similarity
Metal binding3421Magnesium 1 By similarity
Metal binding4391Magnesium 2 By similarity
Binding site2871ATP By similarity
Binding site2941ATP By similarity
Binding site3101ATP By similarity
Binding site4441ATP By similarity
Binding site4571ATP By similarity
Binding site4631ATP By similarity
Site371Interaction with target DNA By similarity
Site3111Interaction with target DNA By similarity
Site4891Interaction with target DNA By similarity
Site5141Interaction with target DNA By similarity

Natural variations

Alternative sequence208 – 2103VRG → RMP in isoform b.
VSP_013778
Alternative sequence211 – 773563Missing in isoform b.
VSP_013779

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified May 24, 2005. Version 2.
Checksum: 458B676F9FCE0BA0

FASTA77386,310
        10         20         30         40         50         60 
MFFSKAGKAA VEWAKGSKVP YKEFALTLEK IEELSGKKKV DELAQFFTKV LDFSPDDLTA 

        70         80         90        100        110        120 
CVYMSVNQLG PSYEGLELGV AENSLIKAVA KATGRTEGKI KEDLRAKGDL GTVAQQSRSN 

       130        140        150        160        170        180 
QKMLAVPKAL TVPTVFNKLT EIAKLSGTSA MNKKVDAISA LLIACQGIEA RFLVRMLAGK 

       190        200        210        220        230        240 
MRIGLGEQSV LSALGHAFTL SKITDQKVRG DKLDSLKDAN VKRVKTAYCE CPNYNRLIEV 

       250        260        270        280        290        300 
ALTEGVEALV EKCKLSPGIP LKPMLAHPTK GIDEIMRRFR NQTMTCEWKY DGERGQIHKR 

       310        320        330        340        350        360 
EDGQIFIYSR NQENNTTKYP DIIEKISSCI GDGVTSFIVD AEVVAIDEAG LILPFQVLST 

       370        380        390        400        410        420 
RKRKNATDDN GVKVVVFLFD LLYFNGEPLV RKPLRKRREL LRTNFKKIDG SFYFATSVDT 

       430        440        450        460        470        480 
NDTDEINSFF DEAVQNKCEG LMIKTLDTEA TYEISRRSHS WLKMKKDYVD GVGDTLDLVV 

       490        500        510        520        530        540 
MGAYSGVGKR TGVYGGYLLG CYNPTTEEYE SVCKIGTGFT DEDLAEQYKI LQDKKIDKSP 

       550        560        570        580        590        600 
SYYQFDHTLK PDDTFSPYLV FEVKCADITI SPRHKAASGL TDDGKGISLR FPRFLRIRDD 

       610        620        630        640        650        660 
KNSDDATSSE QVLEMYKNQE AFANQKIEKA DAVDEDDEFE EKEDEEEELN MTNVSEGSSK 

       670        680        690        700        710        720 
ENPVKEEIKK ETPKSVSPKK FEKKPPVKSS PVNKSPVKSS PIKKEAEKKK GPVASIFSSS 

       730        740        750        760        770 
TKKNEKDVKV ESPSPIRKKK LPSDSDESDE ETSTNKKQPA KKRSRVAIDS DSD

« Hide

Isoform b [UniParc].

Checksum: 7384B3AB04FDDF68
Show »

FASTA21022,716

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z73970 Genomic DNA. Translation: CAA98242.2.
Z73970 Genomic DNA. Translation: CAD44112.2.
PIRT19544.
RefSeqNP_741625.2. NM_171537.3.
NP_741626.2. NM_171538.3.

3D structure databases

ProteinModelPortalQ27474.
SMRQ27474. Positions 13-619.
ModBaseSearch...

Protein-protein interaction databases

STRING6239.C29A12.3a.

Proteomic databases

PaxDbQ27474.
PRIDEQ27474.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC29A12.3a; C29A12.3a; C29A12.3.
GeneID179502.
KEGGcel:CELE_C29A12.3.
UCSCC29A12.3a. c. elegans.

Organism-specific databases

CTD179502.
WormBaseC29A12.3a; CE37480; WBGene00002985; lig-1.
C29A12.3b; CE37481; WBGene00002985; lig-1.

Phylogenomic databases

eggNOGCOG1793.
GeneTreeENSGT00700000104556.
HOGENOMHOG000036006.
InParanoidQ27474.
KOK10747.
OMAAQVACLY.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio905678.

Entry information

Entry nameDNLI1_CAEEL
AccessionPrimary (citable) accession number: Q27474
Secondary accession number(s): Q8MQB3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2005
Last modified: May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents