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Q27474

- DNLI1_CAEEL

UniProt

Q27474 - DNLI1_CAEEL

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Protein
DNA ligase 1
Gene
lig-1, C29A12.3
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei37 – 371Interaction with target DNA By similarity
Binding sitei287 – 2871ATP By similarity
Active sitei289 – 2891N6-AMP-lysine intermediate By similarity
Binding sitei294 – 2941ATP By similarity
Binding sitei310 – 3101ATP By similarity
Sitei311 – 3111Interaction with target DNA By similarity
Metal bindingi342 – 3421Magnesium 1 By similarity
Metal bindingi439 – 4391Magnesium 2 By similarity
Binding sitei444 – 4441ATP By similarity
Binding sitei457 – 4571ATP By similarity
Binding sitei463 – 4631ATP By similarity
Sitei489 – 4891Interaction with target DNA By similarity
Sitei514 – 5141Interaction with target DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. DNA ligation Source: RefGenome
  2. DNA ligation involved in DNA repair Source: InterPro
  3. DNA recombination Source: UniProtKB-KW
  4. cell cycle Source: UniProtKB-KW
  5. cell division Source: UniProtKB-KW
  6. lagging strand elongation Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:lig-1
ORF Names:C29A12.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiC29A12.3a; CE37480; WBGene00002985; lig-1.
C29A12.3b; CE37481; WBGene00002985; lig-1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromosome Source: RefGenome
  2. mitochondrion Source: RefGenome
  3. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773DNA ligase 1
PRO_0000059583Add
BLAST

Proteomic databases

PaxDbiQ27474.
PRIDEiQ27474.

Interactioni

Protein-protein interaction databases

STRINGi6239.C29A12.3a.

Structurei

3D structure databases

ProteinModelPortaliQ27474.
SMRiQ27474. Positions 13-619.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 18910Interaction with target DNA By similarity
Regioni362 – 3643Interaction with target DNA By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi638 – 64811Poly-Glu
Add
BLAST
Compositional biasi660 – 762103Lys-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1793.
GeneTreeiENSGT00750000117745.
HOGENOMiHOG000036006.
InParanoidiQ27474.
KOiK10747.
OMAiLDPSGYN.
OrthoDBiEOG7RFTGN.
PhylomeDBiQ27474.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q27474-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MFFSKAGKAA VEWAKGSKVP YKEFALTLEK IEELSGKKKV DELAQFFTKV    50
LDFSPDDLTA CVYMSVNQLG PSYEGLELGV AENSLIKAVA KATGRTEGKI 100
KEDLRAKGDL GTVAQQSRSN QKMLAVPKAL TVPTVFNKLT EIAKLSGTSA 150
MNKKVDAISA LLIACQGIEA RFLVRMLAGK MRIGLGEQSV LSALGHAFTL 200
SKITDQKVRG DKLDSLKDAN VKRVKTAYCE CPNYNRLIEV ALTEGVEALV 250
EKCKLSPGIP LKPMLAHPTK GIDEIMRRFR NQTMTCEWKY DGERGQIHKR 300
EDGQIFIYSR NQENNTTKYP DIIEKISSCI GDGVTSFIVD AEVVAIDEAG 350
LILPFQVLST RKRKNATDDN GVKVVVFLFD LLYFNGEPLV RKPLRKRREL 400
LRTNFKKIDG SFYFATSVDT NDTDEINSFF DEAVQNKCEG LMIKTLDTEA 450
TYEISRRSHS WLKMKKDYVD GVGDTLDLVV MGAYSGVGKR TGVYGGYLLG 500
CYNPTTEEYE SVCKIGTGFT DEDLAEQYKI LQDKKIDKSP SYYQFDHTLK 550
PDDTFSPYLV FEVKCADITI SPRHKAASGL TDDGKGISLR FPRFLRIRDD 600
KNSDDATSSE QVLEMYKNQE AFANQKIEKA DAVDEDDEFE EKEDEEEELN 650
MTNVSEGSSK ENPVKEEIKK ETPKSVSPKK FEKKPPVKSS PVNKSPVKSS 700
PIKKEAEKKK GPVASIFSSS TKKNEKDVKV ESPSPIRKKK LPSDSDESDE 750
ETSTNKKQPA KKRSRVAIDS DSD 773

Note: No experimental confirmation available.

Length:773
Mass (Da):86,310
Last modified:May 24, 2005 - v2
Checksum:i458B676F9FCE0BA0
GO
Isoform b (identifier: Q27474-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     208-210: VRG → RMP
     211-773: Missing.

Note: No experimental confirmation available.

Show »
Length:210
Mass (Da):22,716
Checksum:i7384B3AB04FDDF68
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei208 – 2103VRG → RMP in isoform b.
VSP_013778
Alternative sequencei211 – 773563Missing in isoform b.
VSP_013779Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73970 Genomic DNA. Translation: CAA98242.2.
Z73970 Genomic DNA. Translation: CAD44112.2.
PIRiT19544.
RefSeqiNP_741625.2. NM_171537.3. [Q27474-1]
NP_741626.2. NM_171538.3. [Q27474-2]
UniGeneiCel.23475.

Genome annotation databases

EnsemblMetazoaiC29A12.3a; C29A12.3a; WBGene00002985. [Q27474-1]
GeneIDi179502.
KEGGicel:CELE_C29A12.3.
UCSCiC29A12.3a. c. elegans. [Q27474-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73970 Genomic DNA. Translation: CAA98242.2 .
Z73970 Genomic DNA. Translation: CAD44112.2 .
PIRi T19544.
RefSeqi NP_741625.2. NM_171537.3. [Q27474-1 ]
NP_741626.2. NM_171538.3. [Q27474-2 ]
UniGenei Cel.23475.

3D structure databases

ProteinModelPortali Q27474.
SMRi Q27474. Positions 13-619.
ModBasei Search...

Protein-protein interaction databases

STRINGi 6239.C29A12.3a.

Proteomic databases

PaxDbi Q27474.
PRIDEi Q27474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C29A12.3a ; C29A12.3a ; WBGene00002985 . [Q27474-1 ]
GeneIDi 179502.
KEGGi cel:CELE_C29A12.3.
UCSCi C29A12.3a. c. elegans. [Q27474-1 ]

Organism-specific databases

CTDi 179502.
WormBasei C29A12.3a ; CE37480 ; WBGene00002985 ; lig-1.
C29A12.3b ; CE37481 ; WBGene00002985 ; lig-1.

Phylogenomic databases

eggNOGi COG1793.
GeneTreei ENSGT00750000117745.
HOGENOMi HOG000036006.
InParanoidi Q27474.
KOi K10747.
OMAi LDPSGYN.
OrthoDBi EOG7RFTGN.
PhylomeDBi Q27474.

Miscellaneous databases

NextBioi 905678.
PROi Q27474.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProi IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiDNLI1_CAEEL
AccessioniPrimary (citable) accession number: Q27474
Secondary accession number(s): Q8MQB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi