Reviewed, UniProtKB/Swiss-Prot Q27474 (DNLI1_CAEEL)

Names and origin

Protein names

Recommended name:
    DNA ligase 1
    EC=6.5.1.1
Alternative name(s):
    DNA ligase I
    Polydeoxyribonucleotide synthase [ATP] 1

Gene names

Name:	lig-1
ORF Names:	C29A12.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q27474-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform b (identifier: Q27474-2)

The sequence of this isoform differs from the canonical sequence as follows:
     208-210: VRG → RMP
     211-773: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	DNA ligase 1		PRO_0000059583
Regions
	Compositional bias	638 – 648	11	Poly-Glu
	Compositional bias	660 – 762	103	Lys-rich
Sites
	Active site	289	1	N6-AMP-lysine intermediate By similarity
Natural variations
	Alternative sequence	208 – 210	3	VRG → RMP in isoform b.		VSP_013778
	Alternative sequence	211 – 773	563	Missing in isoform b.		VSP_013779

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified May 24, 2005. Version 2. Checksum: 458B676F9FCE0BA0	FASTA	773	86,310
	10 20 30 40 50 60 MFFSKAGKAA VEWAKGSKVP YKEFALTLEK IEELSGKKKV DELAQFFTKV LDFSPDDLTA 70 80 90 100 110 120 CVYMSVNQLG PSYEGLELGV AENSLIKAVA KATGRTEGKI KEDLRAKGDL GTVAQQSRSN 130 140 150 160 170 180 QKMLAVPKAL TVPTVFNKLT EIAKLSGTSA MNKKVDAISA LLIACQGIEA RFLVRMLAGK 190 200 210 220 230 240 MRIGLGEQSV LSALGHAFTL SKITDQKVRG DKLDSLKDAN VKRVKTAYCE CPNYNRLIEV 250 260 270 280 290 300 ALTEGVEALV EKCKLSPGIP LKPMLAHPTK GIDEIMRRFR NQTMTCEWKY DGERGQIHKR 310 320 330 340 350 360 EDGQIFIYSR NQENNTTKYP DIIEKISSCI GDGVTSFIVD AEVVAIDEAG LILPFQVLST 370 380 390 400 410 420 RKRKNATDDN GVKVVVFLFD LLYFNGEPLV RKPLRKRREL LRTNFKKIDG SFYFATSVDT 430 440 450 460 470 480 NDTDEINSFF DEAVQNKCEG LMIKTLDTEA TYEISRRSHS WLKMKKDYVD GVGDTLDLVV 490 500 510 520 530 540 MGAYSGVGKR TGVYGGYLLG CYNPTTEEYE SVCKIGTGFT DEDLAEQYKI LQDKKIDKSP 550 560 570 580 590 600 SYYQFDHTLK PDDTFSPYLV FEVKCADITI SPRHKAASGL TDDGKGISLR FPRFLRIRDD 610 620 630 640 650 660 KNSDDATSSE QVLEMYKNQE AFANQKIEKA DAVDEDDEFE EKEDEEEELN MTNVSEGSSK 670 680 690 700 710 720 ENPVKEEIKK ETPKSVSPKK FEKKPPVKSS PVNKSPVKSS PIKKEAEKKK GPVASIFSSS 730 740 750 760 770 TKKNEKDVKV ESPSPIRKKK LPSDSDESDE ETSTNKKQPA KKRSRVAIDS DSD
	Isoform b [UniParc]. Checksum: 7384B3AB04FDDF68		210	22,716
	10 20 30 40 50 60 MFFSKAGKAA VEWAKGSKVP YKEFALTLEK IEELSGKKKV DELAQFFTKV LDFSPDDLTA 70 80 90 100 110 120 CVYMSVNQLG PSYEGLELGV AENSLIKAVA KATGRTEGKI KEDLRAKGDL GTVAQQSRSN 130 140 150 160 170 180 QKMLAVPKAL TVPTVFNKLT EIAKLSGTSA MNKKVDAISA LLIACQGIEA RFLVRMLAGK 190 200 210 MRIGLGEQSV LSALGHAFTL SKITDQKRMP

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73970 Genomic DNA. Translation: CAA98242.2. Z73970 Genomic DNA. Translation: CAD44112.2.
PIR	T19544.
RefSeq	NP_741625.2.
UniGene	Cel.23475
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	C29A12.3. Caenorhabditis elegans. [Contig view]
GeneID	179502.
KEGG	cel:C29A12.3.
NMPDR	fig|6239.3.peg.19533.
Organism-specific databases
WormBase	WBGene00002985. lig-1.
WormPep	C29A12.3a. CE37480. [WorfDB] C29A12.3b. CE37481. [WorfDB]
Gene expression databases
ArrayExpress	Q27474.
Family and domain databases
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	905678.

Entry information

Entry name

DNLI1_CAEEL

Accession

Primary (citable) accession number: Q27474
Secondary accession number(s): Q8MQB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 24, 2005
Last modified:	November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project