Reviewed,
UniProtKB/Swiss-Prot Q27474 (DNLI1_CAEEL)
Last modified
June 16, 2009.
Version 65.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: DNA ligase 1 EC=6.5.1.1 Alternative name(s): DNA ligase I Polydeoxyribonucleotide synthase [ATP] 1 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans [Complete proteome] | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 773 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. |
| Catalytic activity | ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). |
| Cofactor | Magnesium By similarity. |
| Subcellular location | Nucleus By similarity. |
| Sequence similarities | Belongs to the ATP-dependent DNA ligase family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform a (identifier: Q27474-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform b (identifier: Q27474-2) The sequence of this isoform differs from the canonical sequence as follows: 208-210: VRG → RMP 211-773: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 773 | 773 | DNA ligase 1 | PRO_0000059583 | |||||
Regions | |||||||||
| Region | 180 – 189 | 10 | Interaction with target DNA By similarity | ||||||
| Region | 362 – 364 | 3 | Interaction with target DNA By similarity | ||||||
| Compositional bias | 638 – 648 | 11 | Poly-Glu | ||||||
| Compositional bias | 660 – 762 | 103 | Lys-rich | ||||||
Sites | |||||||||
| Active site | 289 | 1 | N6-AMP-lysine intermediate By similarity | ||||||
| Metal binding | 342 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 439 | 1 | Magnesium 2 By similarity | ||||||
| Binding site | 287 | 1 | ATP By similarity | ||||||
| Binding site | 294 | 1 | ATP By similarity | ||||||
| Binding site | 310 | 1 | ATP By similarity | ||||||
| Binding site | 444 | 1 | ATP By similarity | ||||||
| Binding site | 457 | 1 | ATP By similarity | ||||||
| Binding site | 463 | 1 | ATP By similarity | ||||||
| Site | 37 | 1 | Interaction with target DNA By similarity | ||||||
| Site | 311 | 1 | Interaction with target DNA By similarity | ||||||
| Site | 489 | 1 | Interaction with target DNA By similarity | ||||||
| Site | 514 | 1 | Interaction with target DNA By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 208 – 210 | 3 | VRG → RMP in isoform b. | VSP_013778 | |||||
| Alternative sequence | 211 – 773 | 563 | Missing in isoform b. | VSP_013779 | |||||
Sequences
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References
| [1] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
Cross-references
Sequence databases | |
|---|---|
| Z73970 Genomic DNA. Translation: CAA98242.2. Z73970 Genomic DNA. Translation: CAD44112.2. | |
| PIR | T19544. |
| RefSeq | NP_741625.2. |
| UniGene | Cel.23475 |
3D structure databases | |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q27474. |
Genome annotation databases | |
| Ensembl | C29A12.3. Caenorhabditis elegans. [Contig view] |
| GeneID | 179502. |
| KEGG | cel:C29A12.3. |
| NMPDR | fig|6239.3.peg.19533. |
Organism-specific databases | |
| WormBase | WBGene00002985. lig-1. |
| WormPep | C29A12.3a. CE37480. [WorfDB] C29A12.3b. CE37481. [WorfDB] |
Phylogenomic databases | |
| OMA | Q27474. RNWLKLK. |
Enzyme and pathway databases | |
| BRENDA | 6.5.1.1. 672. |
Gene expression databases | |
| ArrayExpress | Q27474. |
Family and domain databases | |
| InterPro | IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view] |
| Gene3D | G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. |
| Pfam | PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00574. dnl1. 1 hit. |
| PROSITE | PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 905678. |
Entry information
| Entry name | DNLI1_CAEEL | ||||||||
| Accession | Primary (citable) accession number: Q27474 Secondary accession number(s): Q8MQB3 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| SIMILARITY comments Index of protein domains and families |
