Q27459 (ACE1_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylcholinesterase 1
Short name=AChE 1
EC=3.1.1.7

Gene names

Name:	ace-1
ORF Names:	CBG16374

Organism

Caenorhabditis briggsae

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Rapidly hydrolyzes choline released into the synapse.
Catalytic activity	Acetylcholine + H2O = choline + acetate.
Subunit structure	Oligomer composed of disulfide-linked homodimers By similarity.
Subcellular location	Cell junction › synapse By similarity. Secreted By similarity. Cell membrane; Peripheral membrane protein By similarity. Note: May be secreted or membrane associated via a non-catalytic subunit By similarity.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Secreted Synapse
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylcholinesterase activity Inferred from electronic annotation. Source: EC carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 620	589	Acetylcholinesterase 1		PRO_0000008609
Sites
	Active site	216	1	Acyl-ester intermediate By similarity
	Active site	346	1	Charge relay system By similarity
	Active site	468	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Glycosylation	486	1	N-linked (GlcNAc...) Potential
	Glycosylation	536	1	N-linked (GlcNAc...) Potential
	Disulfide bond	82 ↔ 109		By similarity
	Disulfide bond	270 ↔ 286		By similarity
	Disulfide bond	430 ↔ 558		By similarity
	Disulfide bond	618		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27459 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 69D73CD3996E11FC	FASTA	620	71,501
	10 20 30 40 50 60 MRYSLLFFIF LPCVITAVDL IHLHDGSPLF GEEVLSQTGK PLTRFLGIPF AEPPIGNLRF 70 80 90 100 110 120 RKPKPKQPWR IPFNATTPPN SCIQSEDTYF GDFYGSTMWN PNTKLSEDCL YLNVYVPGKV 130 140 150 160 170 180 DPNKKLAVMI WVYGGGFWSG TSTLDVYDGR ILTVEENVIL VAMNYRVSIF GFLYMNRSEA 190 200 210 220 230 240 PGNMGMWDQL LAMKWVHKNI DLFGGDTSRI TLFGESAGAA SVSIHMLSQK SAPYFHRAII 250 260 270 280 290 300 QSGSATSPWA IEPRDVALAR AVILYNAMKC GNMSLISPDY DRILDCFQRA DADALRENEW 310 320 330 340 350 360 APVREFGDFP WVPVVDGDFL LENAQTSLKQ GNFKKTQLLA GSNRDESIYF LTYQLPDIFP 370 380 390 400 410 420 VADFFSKSEF IKDRQTWIKG VKDLLPRQIL KCQLTLAAVL HEYEPQDLPI SAQNWLNAMD 430 440 450 460 470 480 KMLGDYHFTC SVNEMALAHT KHGGDTFYYY FTHRATQQTW PEWMGVLHGY EINFIFGEPF 490 500 510 520 530 540 NQKRFNYTDE ERELSNRFMR YWANFAKTGD PNKNEDGSFT QDIWPKYNSV SMEYMNMTVE 550 560 570 580 590 600 SSYPGQNRIG HGPRRKECAF WKAYLPNLMA AVADVGDPYL VWKQQMDKWQ NEYITDWQYH 610 620 FEQYKRYQTY RQSDSETCGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence comparison of ACE-1, the gene encoding acetylcholinesterase of class A, in the two nematodes Caenorhabditis elegans and Caenorhabditis briggsae." Grauso M., Culetto E., Berge J.-B., Toutant J.-P., Arpagaus M. DNA Seq. 6:217-227(1996) [PubMed: 8912924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41846 Genomic DNA. Translation: AAB41269.1. CAAC02000532 Genomic DNA. Translation: CAP34096.3.
RefSeq	XP_002643631.1. XM_002643585.1.
3D structure databases
ProteinModelPortal	Q27459.
ModBase	Search...
Protein family/group databases
MEROPS	S09.979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8585625.
KEGG	cbr:CBG16374.
Organism-specific databases
CTD	8585625.
WormBase	CBG16374; CBP03902; WBGene00036330.
Phylogenomic databases
HOGENOM	HBG716688.
OMA	SILFHYM.
PhylomeDB	Q27459.
Family and domain databases
InterPro	IPR014788. AChE_tetra. IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
KO	K01049.
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
ProDom	PD415333. AChE_tetra. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACE1_CAEBR

Accession

Primary (citable) accession number: Q27459
Secondary accession number(s): A8XNK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project