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Q27452

- PRP2_BOMMO

UniProt

Q27452 - PRP2_BOMMO

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Protein
Phenoloxidase subunit 2
Gene
N/A
Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.1 Publication

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Binds 2 copper ions per subunit By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131Copper A By similarityBy similarity
Metal bindingi217 – 2171Copper A By similarityBy similarity
Metal bindingi243 – 2431Copper A By similarityBy similarity
Metal bindingi366 – 3661Copper B By similarityBy similarity
Metal bindingi370 – 3701Copper B By similarityBy similarity
Metal bindingi406 – 4061Copper B By similarityBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. defense response Source: UniProtKB
  2. melanin biosynthetic process from tyrosine Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenoloxidase subunit 2 (EC:1.14.18.1)
Alternative name(s):
PO 2
Tyrosinase 2
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 51511 Publication
PRO_0000035901Add
BLAST
Chaini52 – 693642Phenoloxidase subunit 21 Publication
PRO_0000035902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi64 – 641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi462 – 4621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi494 – 4941N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi583 ↔ 627 By similarity
Disulfide bondi585 ↔ 634 By similarity
Glycosylationi680 – 6801N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The N-terminus is blocked.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Synthesized by hemocytes and released into the hemolymph plasma.1 Publication

Interactioni

Subunit structurei

Heterodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ27452.

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.

Phylogenomic databases

OMAiIDEMERW.
OrthoDBiEOG7XPZ54.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27452-1 [UniParc]FASTAAdd to Basket

« Hide

MADVFESLEL LFDRPNEPLI TPKGENNSVF QLTEQFLTED YANNGIELNN    50
RFGDDASEKI PLKNLSKLPE FKIATQLPKD AEFSLFLPKH QEMANELLGV 100
LMDVPENELQ DLLSTCAFAR VNLNPQLFNY CYSVALMHRR DTRKVRVKNF 150
AEVFPSKFLD SQVFTQARET AAVIPPDVPR IPIIIPRDYT ATDLEEEHRL 200
AYWREDIGIN LHHYHWHLVY PFTANDLSIV AKDRRGELFF YMHQQVIARF 250
NCERLCNSLK RVKKFSNWRE PIPEAYFPKL DSLTSSRGWP PRQSGMQWQD 300
LNRAAEGLFV TIDEMERWRR NVEEAIATGT VRLPNGQTRP LDIDTLGNML 350
ESSALSPNRE LYGSIHNNGH SFTAYMHDPE HRYLEQFGVI ADEATTMRDP 400
FFYRWHAYID DVFQKHKESA YVRPYTRSEL ENPGVQVRSV SVETPGGQPN 450
TLNTFWMLSD VNLSRGLDFS DNGPVYARFT HLNYRHFSYR INVNNTGSSR 500
RTTVRIFITP KFDERNVPWI FSDQRKMCIE MDRFVTVLNA GENNIVRQST 550
ESSITIPFEQ TFRDLSAQGN DPRRDELATF NYCGCGWPQH MLVPKGTEAG 600
MPFQLFVMLS NYDLDRIDQD DGKQLTCVEA SSFCGLKDKK YPDRRAMGFP 650
FDRPSSSATS LQDFILPNMG LQDITIQLQN VTEPNPRNPP MSV 693
Length:693
Mass (Da):80,119
Last modified:January 1, 1999 - v2
Checksum:i013114DF4CC1A926
GO

Mass spectrometryi

Molecular mass is 81105 Da from positions 52 - 693. Determined by MALDI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331P → Q AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49371 mRNA. Translation: BAA08369.1.
AB048762 mRNA. Translation: BAB41101.1.
RefSeqiNP_001037534.1. NM_001044069.1.
UniGeneiBmo.1389.

Genome annotation databases

EnsemblMetazoaiBGIBMGA013115-RA; BGIBMGA013115-TA; BGIBMGA013115.
GeneIDi693073.
KEGGibmor:693073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49371 mRNA. Translation: BAA08369.1 .
AB048762 mRNA. Translation: BAB41101.1 .
RefSeqi NP_001037534.1. NM_001044069.1.
UniGenei Bmo.1389.

3D structure databases

ProteinModelPortali Q27452.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai BGIBMGA013115-RA ; BGIBMGA013115-TA ; BGIBMGA013115 .
GeneIDi 693073.
KEGGi bmor:693073.

Organism-specific databases

CTDi 693073.

Phylogenomic databases

OMAi IDEMERW.
OrthoDBi EOG7XPZ54.

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProi IPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
PANTHERi PTHR11511. PTHR11511. 1 hit.
Pfami PF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view ]
PRINTSi PR00187. HAEMOCYANIN.
SUPFAMi SSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin."
    Kawabata T., Yasuhara Y., Ochiai M., Matsuura S., Ashida M.
    Proc. Natl. Acad. Sci. U.S.A. 92:7774-7778(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Kinshu X Showa.
    Tissue: Hemocyte.
  2. Asano T., Ashida M.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Reexamination of properties of phenoloxidase isolated from larval hemolymph of the silkworm Bombyx mori."
    Yasuhara Y., Koizumi Y., Katagiri C., Ashida M.
    Arch. Biochem. Biophys. 320:14-23(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
    Tissue: Hemocyte.

Entry informationi

Entry nameiPRP2_BOMMO
AccessioniPrimary (citable) accession number: Q27452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 1, 1999
Last modified: June 11, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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