Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q27452

- PRP2_BOMMO

UniProt

Q27452 - PRP2_BOMMO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phenoloxidase subunit 2

Gene
N/A
Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131Copper ABy similarity
Metal bindingi217 – 2171Copper ABy similarity
Metal bindingi243 – 2431Copper ABy similarity
Metal bindingi366 – 3661Copper BBy similarity
Metal bindingi370 – 3701Copper BBy similarity
Metal bindingi406 – 4061Copper BBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: UniProtKB

GO - Biological processi

  1. defense response Source: UniProtKB
  2. melanin biosynthetic process from tyrosine Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenoloxidase subunit 2 (EC:1.14.18.1)
Alternative name(s):
PO 2
Tyrosinase 2
OrganismiBombyx mori (Silk moth)Imported
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 51511 PublicationPRO_0000035901Add
BLAST
Chaini52 – 693642Phenoloxidase subunit 2PRO_0000035902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi462 – 4621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi494 – 4941N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi583 ↔ 627By similarity
Disulfide bondi585 ↔ 634By similarity
Glycosylationi680 – 6801N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The N-terminus is blocked.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Synthesized by hemocytes and released into the hemolymph plasma.1 Publication

Interactioni

Subunit structurei

Heterodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ27452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Phylogenomic databases

InParanoidiQ27452.
OMAiIDEMERW.
OrthoDBiEOG7XPZ54.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27452-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADVFESLEL LFDRPNEPLI TPKGENNSVF QLTEQFLTED YANNGIELNN
60 70 80 90 100
RFGDDASEKI PLKNLSKLPE FKIATQLPKD AEFSLFLPKH QEMANELLGV
110 120 130 140 150
LMDVPENELQ DLLSTCAFAR VNLNPQLFNY CYSVALMHRR DTRKVRVKNF
160 170 180 190 200
AEVFPSKFLD SQVFTQARET AAVIPPDVPR IPIIIPRDYT ATDLEEEHRL
210 220 230 240 250
AYWREDIGIN LHHYHWHLVY PFTANDLSIV AKDRRGELFF YMHQQVIARF
260 270 280 290 300
NCERLCNSLK RVKKFSNWRE PIPEAYFPKL DSLTSSRGWP PRQSGMQWQD
310 320 330 340 350
LNRAAEGLFV TIDEMERWRR NVEEAIATGT VRLPNGQTRP LDIDTLGNML
360 370 380 390 400
ESSALSPNRE LYGSIHNNGH SFTAYMHDPE HRYLEQFGVI ADEATTMRDP
410 420 430 440 450
FFYRWHAYID DVFQKHKESA YVRPYTRSEL ENPGVQVRSV SVETPGGQPN
460 470 480 490 500
TLNTFWMLSD VNLSRGLDFS DNGPVYARFT HLNYRHFSYR INVNNTGSSR
510 520 530 540 550
RTTVRIFITP KFDERNVPWI FSDQRKMCIE MDRFVTVLNA GENNIVRQST
560 570 580 590 600
ESSITIPFEQ TFRDLSAQGN DPRRDELATF NYCGCGWPQH MLVPKGTEAG
610 620 630 640 650
MPFQLFVMLS NYDLDRIDQD DGKQLTCVEA SSFCGLKDKK YPDRRAMGFP
660 670 680 690
FDRPSSSATS LQDFILPNMG LQDITIQLQN VTEPNPRNPP MSV
Length:693
Mass (Da):80,119
Last modified:January 1, 1999 - v2
Checksum:i013114DF4CC1A926
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331P → Q AA sequence (PubMed:7644494)Curated

Mass spectrometryi

Molecular mass is 81105 Da from positions 52 - 693. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49371 mRNA. Translation: BAA08369.1.
AB048762 mRNA. Translation: BAB41101.1.
RefSeqiNP_001037534.1. NM_001044069.1.
UniGeneiBmo.1389.

Genome annotation databases

EnsemblMetazoaiBGIBMGA013115-RA; BGIBMGA013115-TA; BGIBMGA013115.
GeneIDi693073.
KEGGibmor:693073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49371 mRNA. Translation: BAA08369.1 .
AB048762 mRNA. Translation: BAB41101.1 .
RefSeqi NP_001037534.1. NM_001044069.1.
UniGenei Bmo.1389.

3D structure databases

ProteinModelPortali Q27452.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai BGIBMGA013115-RA ; BGIBMGA013115-TA ; BGIBMGA013115 .
GeneIDi 693073.
KEGGi bmor:693073.

Organism-specific databases

CTDi 35910.

Phylogenomic databases

InParanoidi Q27452.
OMAi IDEMERW.
OrthoDBi EOG7XPZ54.

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProi IPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
PANTHERi PTHR11511. PTHR11511. 1 hit.
Pfami PF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view ]
PRINTSi PR00187. HAEMOCYANIN.
SUPFAMi SSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin."
    Kawabata T., Yasuhara Y., Ochiai M., Matsuura S., Ashida M.
    Proc. Natl. Acad. Sci. U.S.A. 92:7774-7778(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Kinshu X Showa1 Publication.
    Tissue: Hemocyte1 Publication.
  2. Asano T., Ashida M.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Reexamination of properties of phenoloxidase isolated from larval hemolymph of the silkworm Bombyx mori."
    Yasuhara Y., Koizumi Y., Katagiri C., Ashida M.
    Arch. Biochem. Biophys. 320:14-23(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
    Tissue: Hemocyte1 Publication.

Entry informationi

Entry nameiPRP2_BOMMO
AccessioniPrimary (citable) accession number: Q27452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 1, 1999
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3