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Protein

Phenoloxidase subunit 1

Gene
N/A
Organism
Bombyx mori (Silk moth)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Cu2+By similarityNote: Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi209Copper ABy similarity1
Metal bindingi213Copper ABy similarity1
Metal bindingi239Copper ABy similarity1
Metal bindingi366Copper BBy similarity1
Metal bindingi370Copper BBy similarity1
Metal bindingi406Copper BBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • monophenol monooxygenase activity Source: UniProtKB

GO - Biological processi

  • defense response Source: UniProtKB
  • melanin biosynthetic process from tyrosine Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenoloxidase subunit 1 (EC:1.14.18.1)
Alternative name(s):
PO 1
Tyrosinase 1
OrganismiBombyx mori (Silk moth)Imported
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
Proteomesi
  • UP000005204 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000358991 – 511 PublicationAdd BLAST51
ChainiPRO_000003590052 – 685Phenoloxidase subunit 1Add BLAST634

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi254N-linked (GlcNAc...)Sequence analysis1
Glycosylationi324N-linked (GlcNAc...)Sequence analysis1
Glycosylationi491N-linked (GlcNAc...)Sequence analysis1
Glycosylationi540N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi581 ↔ 623By similarity
Disulfide bondi583 ↔ 630By similarity

Post-translational modificationi

The N-terminus is blocked.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Synthesized by hemocytes and released into the hemolymph plasma.1 Publication

Interactioni

Subunit structurei

Heterodimer.1 Publication

Protein-protein interaction databases

STRINGi7091.BGIBMGA012763-TA.

Structurei

3D structure databases

ProteinModelPortaliQ27451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Phylogenomic databases

eggNOGiENOG410IKED. Eukaryota.
ENOG4111KTW. LUCA.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDAKNNLLL FFDRPSEPCF MQKGEENAVF EIPDNYYPEK YQRVSNAIGN
60 70 80 90 100
RFGSDAGRMI PIRNIALPNL DLPMELPYNE QFSLFVPKHR KLAGRLIDIF
110 120 130 140 150
MGMRDVEDLQ SVCSYCQLRI NPYMFNYCLS VAILHRPDTK GLSIPTFAES
160 170 180 190 200
FPDKFMDPKV FRQAREVSSV VPSGARMPIV IPSNYTASDT EPEQRVAYFR
210 220 230 240 250
EDIGINLHHW HWHLVYPFDA ADRAIVNKDR RGELFYYMHQ QIIARYNVER
260 270 280 290 300
MCNNLSRVRR YNNFRAAIEE GYFPKLDSTV ASRAWPPRFA GTTIRDLDRP
310 320 330 340 350
VDQIRSDVSE LETWRDRFLQ AIENMSVMLP NGRQLPLDEE TGIDVLGNLM
360 370 380 390 400
ESSIISRNRP YYGDLHNMGH VFISYSHDPD HRHLEQFGVM GDSATAMRDP
410 420 430 440 450
VFYRWHAYID DIFHLYKYKL TPYGNDRLDF PNIRVSSVSI EGGGTPNTLN
460 470 480 490 500
TLWEQSTVDL GRGMDFTPRG SVLARFTHLQ HDEYNYVIEV NNTGGSSVMG
510 520 530 540 550
MFRIFIAPTV DESGKPFSFD EQRKLMIELD KFSQGVKPGN NTIRRKSIDS
560 570 580 590 600
SVTIPYERTF RNQADRPADP GTAGAAEFDF CGCGWPHHML VPKGTTQGYP
610 620 630 640 650
MVLFVMVSNW NDDRVEQDLV GSCNDAASYC GIRDRKYPDR RAMGFPFDRP
660 670 680
APAATTLSDF LRPNMAVRDC IVRFTDRTRQ RGQQG
Length:685
Mass (Da):78,785
Last modified:January 1, 1999 - v2
Checksum:i9DE93E0760DD353C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171V → A AA sequence (PubMed:7644494).Curated1

Mass spectrometryi

Molecular mass is 78880 Da from positions 52 - 685. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49370 mRNA. Translation: BAA08368.1.
RefSeqiNP_001037335.1. NM_001043870.1.
UniGeneiBmo.344.

Genome annotation databases

GeneIDi692758.
KEGGibmor:692758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49370 mRNA. Translation: BAA08368.1.
RefSeqiNP_001037335.1. NM_001043870.1.
UniGeneiBmo.344.

3D structure databases

ProteinModelPortaliQ27451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7091.BGIBMGA012763-TA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi692758.
KEGGibmor:692758.

Organism-specific databases

CTDi37044.

Phylogenomic databases

eggNOGiENOG410IKED. Eukaryota.
ENOG4111KTW. LUCA.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP1_BOMMO
AccessioniPrimary (citable) accession number: Q27451
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 1, 1999
Last modified: January 20, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.