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Q27451

- PRP1_BOMMO

UniProt

Q27451 - PRP1_BOMMO

Protein

Phenoloxidase subunit 1

Gene
N/A
Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (01 Jan 1999)
      Previous versions | rss
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    Functioni

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi209 – 2091Copper ABy similarity
    Metal bindingi213 – 2131Copper ABy similarity
    Metal bindingi239 – 2391Copper ABy similarity
    Metal bindingi366 – 3661Copper BBy similarity
    Metal bindingi370 – 3701Copper BBy similarity
    Metal bindingi406 – 4061Copper BBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. monophenol monooxygenase activity Source: UniProtKB

    GO - Biological processi

    1. defense response Source: UniProtKB
    2. melanin biosynthetic process from tyrosine Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenoloxidase subunit 1 (EC:1.14.18.1)
    Alternative name(s):
    PO 1
    Tyrosinase 1
    OrganismiBombyx mori (Silk moth)Imported
    Taxonomic identifieri7091 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
    ProteomesiUP000005204: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 51511 PublicationPRO_0000035899Add
    BLAST
    Chaini52 – 685634Phenoloxidase subunit 1PRO_0000035900Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi540 – 5401N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi581 ↔ 623By similarity
    Disulfide bondi583 ↔ 630By similarity

    Post-translational modificationi

    The N-terminus is blocked.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Expressioni

    Tissue specificityi

    Synthesized by hemocytes and released into the hemolymph plasma.1 Publication

    Interactioni

    Subunit structurei

    Heterodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ27451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    1.20.1370.10. 1 hit.
    2.60.40.1520. 1 hit.
    InterProiIPR013788. Hemocyanin/hexamerin.
    IPR000896. Hemocyanin/hexamerin_mid_dom.
    IPR005203. Hemocyanin_C.
    IPR005204. Hemocyanin_N.
    IPR014756. Ig_E-set.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PANTHERiPTHR11511. PTHR11511. 1 hit.
    PfamiPF03723. Hemocyanin_C. 1 hit.
    PF00372. Hemocyanin_M. 1 hit.
    PF03722. Hemocyanin_N. 1 hit.
    [Graphical view]
    PRINTSiPR00187. HAEMOCYANIN.
    SUPFAMiSSF48050. SSF48050. 1 hit.
    SSF48056. SSF48056. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
    PS00210. HEMOCYANIN_2. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27451-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDAKNNLLL FFDRPSEPCF MQKGEENAVF EIPDNYYPEK YQRVSNAIGN    50
    RFGSDAGRMI PIRNIALPNL DLPMELPYNE QFSLFVPKHR KLAGRLIDIF 100
    MGMRDVEDLQ SVCSYCQLRI NPYMFNYCLS VAILHRPDTK GLSIPTFAES 150
    FPDKFMDPKV FRQAREVSSV VPSGARMPIV IPSNYTASDT EPEQRVAYFR 200
    EDIGINLHHW HWHLVYPFDA ADRAIVNKDR RGELFYYMHQ QIIARYNVER 250
    MCNNLSRVRR YNNFRAAIEE GYFPKLDSTV ASRAWPPRFA GTTIRDLDRP 300
    VDQIRSDVSE LETWRDRFLQ AIENMSVMLP NGRQLPLDEE TGIDVLGNLM 350
    ESSIISRNRP YYGDLHNMGH VFISYSHDPD HRHLEQFGVM GDSATAMRDP 400
    VFYRWHAYID DIFHLYKYKL TPYGNDRLDF PNIRVSSVSI EGGGTPNTLN 450
    TLWEQSTVDL GRGMDFTPRG SVLARFTHLQ HDEYNYVIEV NNTGGSSVMG 500
    MFRIFIAPTV DESGKPFSFD EQRKLMIELD KFSQGVKPGN NTIRRKSIDS 550
    SVTIPYERTF RNQADRPADP GTAGAAEFDF CGCGWPHHML VPKGTTQGYP 600
    MVLFVMVSNW NDDRVEQDLV GSCNDAASYC GIRDRKYPDR RAMGFPFDRP 650
    APAATTLSDF LRPNMAVRDC IVRFTDRTRQ RGQQG 685
    Length:685
    Mass (Da):78,785
    Last modified:January 1, 1999 - v2
    Checksum:i9DE93E0760DD353C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti171 – 1711V → A AA sequence (PubMed:7644494)Curated

    Mass spectrometryi

    Molecular mass is 78880 Da from positions 52 - 685. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49370 mRNA. Translation: BAA08368.1.
    RefSeqiNP_001037335.1. NM_001043870.1.
    UniGeneiBmo.344.

    Genome annotation databases

    GeneIDi692758.
    KEGGibmor:692758.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49370 mRNA. Translation: BAA08368.1 .
    RefSeqi NP_001037335.1. NM_001043870.1.
    UniGenei Bmo.344.

    3D structure databases

    ProteinModelPortali Q27451.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 692758.
    KEGGi bmor:692758.

    Organism-specific databases

    CTDi 692758.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    1.20.1370.10. 1 hit.
    2.60.40.1520. 1 hit.
    InterProi IPR013788. Hemocyanin/hexamerin.
    IPR000896. Hemocyanin/hexamerin_mid_dom.
    IPR005203. Hemocyanin_C.
    IPR005204. Hemocyanin_N.
    IPR014756. Ig_E-set.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    PANTHERi PTHR11511. PTHR11511. 1 hit.
    Pfami PF03723. Hemocyanin_C. 1 hit.
    PF00372. Hemocyanin_M. 1 hit.
    PF03722. Hemocyanin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00187. HAEMOCYANIN.
    SUPFAMi SSF48050. SSF48050. 1 hit.
    SSF48056. SSF48056. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00209. HEMOCYANIN_1. 1 hit.
    PS00210. HEMOCYANIN_2. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin."
      Kawabata T., Yasuhara Y., Ochiai M., Matsuura S., Ashida M.
      Proc. Natl. Acad. Sci. U.S.A. 92:7774-7778(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Kinshu X Showa1 Publication.
      Tissue: Hemocyte1 Publication.
    2. "Reexamination of properties of phenoloxidase isolated from larval hemolymph of the silkworm Bombyx mori."
      Yasuhara Y., Koizumi Y., Katagiri C., Ashida M.
      Arch. Biochem. Biophys. 320:14-23(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
      Tissue: Hemocyte1 Publication.

    Entry informationi

    Entry nameiPRP1_BOMMO
    AccessioniPrimary (citable) accession number: Q27451
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: January 1, 1999
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3