Reviewed, UniProtKB/Swiss-Prot Q27450 (CYP1_BRUMA)

Names and origin

Protein names

Recommended name:
    Peptidyl-prolyl cis-trans isomerase 1
      Short name=PPIase 1
    EC=5.2.1.8
Alternative name(s):
    Cyclophilin
    BmCYP-1

Gene names

Name:

CYP-1

Organism

Brugia malayi (Filarial nematode worm)

Taxonomic identifier

6279 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Brugia

Protein attributes

Sequence length	843 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Relatively insensitive to inhibition by cyclosporin A (CsA).
Sequence similarities	Contains 1 PPIase cyclophilin-type domain.

Ontologies

Keywords
Molecular function	Isomerase Rotamase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 843

843

Peptidyl-prolyl cis-trans isomerase 1

PRO_0000064215

Regions

Domain

10 – 175

166

PPIase cyclophilin-type

Compositional bias

700 – 709

10

Poly-Arg

Compositional bias

713 – 716

4

Poly-Arg

Compositional bias

800 – 815

16

Poly-Ser

Compositional bias

828 – 837

10

Poly-Ser

Secondary structure

1

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843

Helix Strand Turn

Details...

Beta strand

7 – 15

9

Beta strand

18 – 27

10

Turn

29 – 31

3

Helix

33 – 44

12

Turn

51 – 53

3

Beta strand

63 – 68

6

Turn

69 – 71

3

Beta strand

72 – 75

4

Turn

78 – 80

3

Beta strand

81 – 84

4

Beta strand

108 – 111

4

Beta strand

123 – 128

6

Helix

131 – 133

3

Turn

134 – 136

3

Beta strand

139 – 145

7

Helix

147 – 154

8

Beta strand

164 – 166

3

Beta strand

168 – 175

8

Sequences

	Sequence		Length	Mass (Da)
	Q27450-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3C34EC90A32EDBDC	FASTA	843	97,818
	10 20 30 40 50 60 MSKKDRRRVF LDVTIDGNLA GRIVMELYND IAPRTCNNFL MLCTGMAGTG KISGKPLHYK 70 80 90 100 110 120 GSTFHRVIKN FMIQGGDFTK GDGTGGESIY GGMFDDEEFV MKHDEPFVVS MANKGPNTNG 130 140 150 160 170 180 SQFFITTTPA PHLNNIHVVF GKVVSGQEVV TKIEYLKTNS KNRPLADVVI LNCGELVRRK 190 200 210 220 230 240 KRQHSSRSNE SVSSSTSTEK SHKKTKKTKM KEKKRKESDE VEQLEIGTVV PEAELQLSSV 250 260 270 280 290 300 KAEDLPDEPD HQNKYLMRRS KTPENSRKGK KEKQRQSPHR FSRRDIGHRL NRMRRTRTGH 310 320 330 340 350 360 KIKGRGALRF RTPEGSSDHD GSRTPPHWRR EQNRVITLDE LHRLQEKRKA YELEELENPK 370 380 390 400 410 420 NDVVDKAKTG ILLNTSEKIE DKEERYRGKS EKKENRHERS RHTTRRSPEH VTRHFVKEKN 430 440 450 460 470 480 RHKVDEVGNS EDMKQTKRDR RGRADEKEKV EVNGEKAAAM DELNLDEPTV EVTLDSAEDI 490 500 510 520 530 540 RDSDDEAIRI HLLKAKKMAE EKTKQEAKML EKTGDKEGRD QKTISEAKQK DSAEKDRQHR 550 560 570 580 590 600 EHKNDELEKR AIEKQDKDQI VERDTGSKQR RKSDSKEHRG KTDRKHRSKS IEEDGRRSTS 610 620 630 640 650 660 REKLDDLKRK ETSGQKSQAD SEQTVEAKTN VVDSNSDNSK MSVNGKLKEV SSTNKENEVS 670 680 690 700 710 720 EQKDLKAEST KSEEIKQQVN EVSRKQKGGE KPKEHKRNER SRSRRRRSRS NGRRRRSSSR 730 740 750 760 770 780 RSRSRDRRHK SRSRSRGYVR RFEGWSRSRR PTRRELYDER MRRERERRRS FDRYSDRRRT 790 800 810 820 830 840 RSRSARRDSD RHSRRSRKRS PSSSSSSSES SSSDSRSTAS SSASSKRSSS SDSSRSSRSR SSN

References

[1]	"Molecular characterization of a cyclosporin A-insensitive cyclophilin from the parasitic nematode Brugia malayi." Page A.P., Landry D., Wilson G.G., Carlow C.K.S. Biochemistry 34:11545-11550(1995) [PubMed: 7547885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The X-ray structure of a divergent cyclophilin from the nematode parasite Brugia malayi." Taylor P., Page A.P., Kontopidis G., Husi H., Walkinshaw M.D. FEBS Lett. 425:361-366(1998) [PubMed: 9559680] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-177.
[3]	"Crystal structure of the cyclophilin-like domain from the parasitic nematode Brugia malayi." Mikol V., Ma D., Carlow C.K.S. Protein Sci. 7:1310-1316(1998) [PubMed: 9655334] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 1-177. Strain: ATCC 75593 / Bmcyp-1.
[4]	"Crystal structure of the complex of Brugia malayi cyclophilin and cyclosporin A." Ellis P.J., Carlow C.K.S., Ma D., Kuhn P. Biochemistry 39:592-598(2000) [PubMed: 10642184] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.47 ANGSTROMS) OF 1-177 IN COMPLEX WITH CYCLOSPORIN A.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L37292 mRNA. Translation: AAC37249.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A33	X-ray	2.15	A	1-177	[»]
1A58	X-ray	1.95	A	1-177	[»]
1C5F	X-ray	2.47	A/C/E/G/I/K/M/O	1-177	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

5.2.1.8. 27.

Family and domain databases

InterPro

IPR002130. PPIase_cyclophilin.
[Graphical view]

Gene3D

G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit.

Pfam

PF00160. Pro_isomerase. 1 hit.
[Graphical view]

PRINTS

PR00153. CSAPPISMRASE.

PROSITE

PS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYP1_BRUMA

Accession

Primary (citable) accession number: Q27450

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)