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Protein

Transcription factor E2f1

Gene

E2f1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to E2f sites. Required for wild-type growth in mitotic and polytene tissues, Contributes to the expression of replication genes at the G1-S transition and Cyclin E. Activates cell proliferation in wing imaginal disk, which requires expression of vg.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi253 – 318Sequence analysisAdd BLAST66

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • DNA binding transcription factor activity Source: FlyBase
  • protein dimerization activity Source: InterPro
  • RNA polymerase II regulatory region DNA binding Source: FlyBase
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • antimicrobial humoral response Source: FlyBase
  • DNA endoreduplication Source: FlyBase
  • eggshell chorion gene amplification Source: FlyBase
  • G1/S transition of mitotic cell cycle Source: FlyBase
  • imaginal disc development Source: FlyBase
  • larval lymph gland hemopoiesis Source: FlyBase
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • nurse cell apoptotic process Source: FlyBase
  • positive regulation of gene expression Source: FlyBase
  • positive regulation of nurse cell apoptotic process Source: FlyBase
  • positive regulation of TORC1 signaling Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • regulation of cell cycle Source: FlyBase
  • regulation of execution phase of apoptosis Source: FlyBase
  • regulation of hemocyte proliferation Source: FlyBase
  • ventral cord development Source: FlyBase

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-68689 CDC6 association with the ORC:origin complex
R-DME-68911 G2 Phase
R-DME-69231 Cyclin D associated events in G1
R-DME-69298 Association of licensing factors with the pre-replicative complex
R-DME-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2f1
Alternative name(s):
dE2F
Gene namesi
Name:E2f1Imported
Synonyms:E2fImported
ORF Names:CG6376Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0011766 E2f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi153 – 156ITNY → ATAA: Abolishes interaction with PCNA and subsequent degradation by the proteasome. 4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194741 – 805Transcription factor E2f1Add BLAST805

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei434Phosphoserine1 Publication1

Post-translational modificationi

Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase. Ubiquitination by the DCX(DTL) complex is essential for cell cycle control and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ27368
PRIDEiQ27368

PTM databases

iPTMnetiQ27368

Expressioni

Tissue specificityi

Segmentally repeated expression throughout early embryos is restricted to the ventral nerve cord in later embryos.1 Publication

Developmental stagei

Throughout embryonic development.1 Publication

Gene expression databases

BgeeiFBgn0011766
ExpressionAtlasiQ27368 differential
GenevisibleiQ27368 DM

Interactioni

Subunit structurei

Heterodimer of E2f and Dp. Cooperates to give sequence-specific DNA binding and optimal trans-activation. Interacts with PCNA.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi67516, 65 interactors
DIPiDIP-21076N
ELMiQ27368
IntActiQ27368, 11 interactors
STRINGi7227.FBpp0083516

Structurei

3D structure databases

ProteinModelPortaliQ27368
SMRiQ27368
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni318 – 411DimerizationSequence analysisAdd BLAST94

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi147 – 161PIP-box K+4 motifAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 19Poly-Ser6
Compositional biasi64 – 68Poly-Asn5
Compositional biasi115 – 125Poly-AlaAdd BLAST11
Compositional biasi129 – 143Gly-richAdd BLAST15
Compositional biasi245 – 249Poly-Ser5
Compositional biasi519 – 573Gly-richAdd BLAST55
Compositional biasi525 – 533Poly-Gln9
Compositional biasi594 – 601Poly-Ala8
Compositional biasi701 – 710Poly-Gly10

Domaini

The PIP-box K+4 motif mediates both the interaction with PCNA and the recruitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.1 Publication

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
InParanoidiQ27368
KOiK06620
OMAiYNCAMED
OrthoDBiEOG091G0BHD
PhylomeDBiQ27368

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

Q27368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFFVNVAP INNSNSSSSH TTTSSNTQRH QQHQQHYGGS GTTGHTMVAR
60 70 80 90 100
RLNYDLHGGT TSINNNNNIV IKNESVDLDY DHVLSSSDSN SNGGVAAHLR
110 120 130 140 150
DHVYISLDKG HNTGAVATAA AAATAGHTQQ QLQQQHHHQN QQQRKATGKS
160 170 180 190 200
NDITNYYKVK RRPHAVSDEI HPKKQAKQSA HHQTVYQKHT ASSAPQQLRH
210 220 230 240 250
SHHQLRHDAD AELDEDVVER VAKPASHHPF SLSTPQQQLA ASVASSSSSG
260 270 280 290 300
DRNRADTSLG ILTKKFVDLL QESPDGVVDL NEASNRLHVQ KRRIYDITNV
310 320 330 340 350
LEGINILEKK SKNNIQWRCG QSMVSQERSR HIEADSLRLE QQENELNKAI
360 370 380 390 400
DLMRENLAEI SQEVENSGGM AYVTQNDLLN VDLFKDQIVI VIKAPPEAKL
410 420 430 440 450
VLPNTKLPRE IYVKAENSGE INVFLCHDTS PENSPIAPGA GYVGAPGAGC
460 470 480 490 500
VRTATSTRLH PLTNQRLNDP LFNNIDAMST KGLFQTPYRS ARNLSKSIEE
510 520 530 540 550
AAKQSQPEYN NICDIAMGQH HNLNQQQQQQ QQQLLQQPEE DDVDVELNQL
560 570 580 590 600
VPTLTNPVVR THQFQQHQQP SIQELFSSLT ESSPPTPTKR RREAAAAAIA
610 620 630 640 650
AGSSTTATTT LNSHNNRNHS NHSNHSNHSS SNNSKSQPPT IGYGSSQRRS
660 670 680 690 700
DVPMYNCAME GATTTSATAD TTAATSRSAA ASSLQMQFAA VAESNNGSSS
710 720 730 740 750
GGGGGGGGYG SIAGAGANAD PHQPYSHDRN SLPPGVADCD ANSNSSSVTL
760 770 780 790 800
QGLDALFNDI GSDYFSNDIA FVSINPPDDN DYPYALNANE GIDRLFDFGS

DAYGP
Length:805
Mass (Da):87,460
Last modified:June 1, 2001 - v2
Checksum:iBD49C813DDB2A77D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127H → Q in CAA55186 (PubMed:8114698).Curated1
Sequence conflicti127H → Q in AAA19003 (PubMed:8022787).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10184 mRNA Translation: AAA19003.1
X78421 mRNA Translation: CAA55186.1
AB011813 Genomic DNA Translation: BAA32746.1
AE014297 Genomic DNA Translation: AAF55904.1
AE014297 Genomic DNA Translation: AAN13878.1
AE014297 Genomic DNA Translation: AAN13879.1
BT016096 mRNA Translation: AAV36981.1
PIRiA56199
RefSeqiNP_001287455.1, NM_001300526.1
NP_524437.2, NM_079713.3
NP_732646.1, NM_169961.3
NP_732647.1, NM_169962.3
UniGeneiDm.7299

Genome annotation databases

EnsemblMetazoaiFBtr0084117; FBpp0083516; FBgn0011766
FBtr0084118; FBpp0083517; FBgn0011766
FBtr0084119; FBpp0083518; FBgn0011766
FBtr0346157; FBpp0311985; FBgn0011766
GeneIDi42550
KEGGidme:Dmel_CG6376
UCSCiCG6376-RB d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiE2F1_DROME
AccessioniPrimary (citable) accession number: Q27368
Secondary accession number(s): O77035, Q5U0Z6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health