Reviewed, UniProtKB/Swiss-Prot Q27367 (CRQ_DROME)

Names and origin

Protein names

Recommended name:
    Protein croquemort
Alternative name(s):
    d-CD36

Gene names

Name:	crq
ORF Names:	CG4280

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Macrophage receptor for apoptotic cells.
Subcellular location	Cell membrane; Single-pass membrane protein.
Tissue specificity	Found on hemocytes from hemolymph, and later in the development, on macrophages that contain apoptotic cell corpses.
Developmental stage	Expressed on hemocytes/macrophages in embryogenesis. First detected in late stage 11 embryos, which corresponds to the first wave of apoptosis. Expressed in cells that by stage 12 spread throughout the embryo and by stage 13/14 have migrated from both ends towards the middle until they are evenly distributed by stage 15.
Post-translational modification	N-glycosylated and/or O-glycosylated Probable.
Miscellaneous	'Croquemort' means literally 'the one who bites dead persons' in French and is colloquial for undertaker.
Sequence similarities	Belongs to the CD36 family.
Sequence caution	The sequence AAF51494.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from mutant phenotype. Source: FlyBase cell adhesion Inferred from electronic annotation. Source: InterPro macrophage activation Traceable author statement. Source: FlyBase phagocytosis triggered by activation of immune response cell surface activating receptor Inferred from direct assay. Source: FlyBase phagocytosis, engulfment Traceable author statement. Source: FlyBase regulation of phagocytosis Traceable author statement. Source: FlyBase salivary gland cell autophagic cell death Inferred from expression pattern. Source: FlyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	scavenger receptor activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Protein croquemort		PRO_0000144164
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Potential
	Topological domain	34 – 457	424	Extracellular Potential
Amino acid modifications
	Lipidation	2	1	S-palmitoyl cysteine Potential
	Lipidation	3	1	S-palmitoyl cysteine Potential
	Lipidation	5	1	S-palmitoyl cysteine Potential
	Lipidation	6	1	S-palmitoyl cysteine Potential
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	110	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Disulfide bond	256 ↔ 324		By similarity
	Disulfide bond	285 ↔ 345		By similarity
	Disulfide bond	326 ↔ 334		By similarity
Experimental info
	Sequence conflict	247	1	S → A in CAA83455. Ref.1
	Sequence conflict	247	1	S → A in CAA83454. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q27367-1 [UniParc]. Last modified November 25, 2002. Version 2. Checksum: CD6FA6CD21A18D61	FASTA	457	52,334
	10 20 30 40 50 60 MCCKCCGETQ RKVWVFGLGS VFLLLGILIV VFWPGIADNL VEDGLTLKPG TDAYESWLEA 70 80 90 100 110 120 PIPIYLSFYM FNWTNPEDIR NPDIKPNFVE MGPYTFLEKH KKENYTFYDN ATVAYYERRT 130 140 150 160 170 180 WFFDPERSNG TLDDMVTAAH AITATVADEM RDQRKIVKKI INFMLNHEGG KLYVTKPVGE 190 200 210 220 230 240 WIFEGYQDNI TDFLNLFNTT KIDIPYKRFG WLADRNESLT YDGLFTIHTG TDDISNLGRL 250 260 270 280 290 300 THWNGKSETG FYEMPCGIVN GTTGDMFPPK MNVNDEITIF ATDACRFMNL RPRGTYENHG 310 320 330 340 350 360 LTATKWVGTE ETLDSGENYP NQACFCDEAR FDECPKTGVV ECKACRDKAP IYSSFPHFYL 370 380 390 400 410 420 ADQSYVDAVS GMKPEKEKHE FFLAVEPITG VPVQVHGRIQ INMMIEPDDD FDIYRGVQKV 430 440 450 LMPMFWFDQY AELSSELASK PSWPLICPVT ESYSAIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Croquemort, a novel Drosophila hemocyte/macrophage receptor that recognizes apoptotic cells." Franc N.C., Dimarcq J.-L., Lagueux M., Hoffmann J., Ezekowitz R.A.B. Immunity 4:431-443(1996) [PubMed: 8630729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster." Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V. Glycobiology 17:1388-1403(2007) [PubMed: 17893096] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104 AND ASN-110, MASS SPECTROMETRY. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z31583 mRNA. Translation: CAA83455.1. Z31582 mRNA. Translation: CAA83454.1. AE014134 Genomic DNA. Translation: AAF51494.1. Sequence problems.
PIR	S43137.
RefSeq	NP_787957.1.
UniGene	Dm.19756
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q27367. 1 interaction.
Genome annotation databases
Ensembl	FBgn0015924. Drosophila melanogaster. [Contig view]
GeneID	33219.
KEGG	dme:Dmel_CG4280.
Organism-specific databases
FlyBase	FBgn0015924. crq.
Gene expression databases
ArrayExpress	Q27367.
GermOnline	CG4280. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002159. CD36. [Graphical view]
PANTHER	PTHR11923. CD36. 1 hit.
Pfam	PF01130. CD36. 1 hit. [Graphical view]
PRINTS	PR01609. CD36FAMILY.
ProtoNet	Search...
Other Resources
NextBio	782496.

Entry information

Entry name

CRQ_DROME

Accession

Primary (citable) accession number: Q27367
Secondary accession number(s): Q9VPQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 25, 2002
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project