Q27333 (NT56_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lethal(2)neighbour of tid protein

Alternative name(s):
NOT45
NOT56

Gene names

Name:	l(2)not
ORF Names:	CG4084

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Developmental stage	Expressed only in embryos.
Miscellaneous	In strains Harvich and Apxo, there are 2 genes which code for l2not protein. They differ in their C-terminus.
Sequence similarities	Belongs to the glycosyltransferase 58 family.
Sequence caution	The sequence AAL29130.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG16947-RA	Q9VMD4	1	EBI-173443,EBI-86395

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Lethal(2)neighbour of tid protein		PRO_0000080568
Regions
	Transmembrane	44 – 64	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Transmembrane	212 – 232	21	Helical; Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Transmembrane	295 – 315	21	Helical; Potential
	Transmembrane	404 – 424	21	Helical; Potential
	Transmembrane	427 – 447	21	Helical; Potential
	Transmembrane	465 – 485	21	Helical; Potential
Natural variations
	Natural variant	286	1	V → L in strain: Berkeley and bIf.
	Natural variant	311	1	I → T in strain: Berkeley, bIf and Apxo.
	Natural variant	333	1	T → A in strain: Berkeley, bIf and Apxo.
	Natural variant	338	1	Q → E in strain: Berkeley, bIf and Apxo.
	Natural variant	340	1	K → N in strain: Berkeley and bIf.
	Natural variant	489	1	I → V in strain: Berkeley, bIf and Apxo.
Experimental info
	Mutagenesis	433	1	H → R in tid4 mutant.

Sequences

	Sequence		Length	Mass (Da)
	Q27333 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E2F7415940465F25	FASTA	510	58,461
	10 20 30 40 50 60 MAPPKAASHR PAVRRKKSGT LVDSILDKYL NVRFFKYLLL EPAALPIVGL FVLLAELVIN 70 80 90 100 110 120 VVVIQRVPYT EIDWVAYMQE CEGFLNGTTN YSLLRGDTGP LVYPAAFVYI YSALYYVTSH 130 140 150 160 170 180 GTNVRLAQYI FAGIYLLQLA LVLRLYSKSR KVPPYVLVLS AFTSYRIHSI YVLRLFNDPV 190 200 210 220 230 240 AVLLLYAALN LFLDRRWTLG STFFSLAVGV KMNILLFAPA LLLFYLANLG LLRTILQLAV 250 260 270 280 290 300 CGVIQLLLGA PFLLTHPVEY LRGSFDLGRI FEHKWTVNYR FLSRDVFENR TFHVSLLGLH 310 320 330 340 350 360 LLLLLAFAKP IWTFFQSYVR LRRIEDQLQP QITQQNLQLK AQKRPKKVEK DKDKDQKKFT 370 380 390 400 410 420 TEQQSFLKAF EKSLQKASGG KATPAPAQAE PERYGIHFDR CTQLALLPFF LCNLVGVACS 430 440 450 460 470 480 RSLHYQFYVW YFHSLPYLAW STPYSLGVRC LILGLIEYCW NTYPSTNFSS AALHFTHIIL 490 500 510 LAGVAKQLIQ TMRINNAAKR EQQEQQKKLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene within gene configuration and expression of the Drosophila melanogaster genes lethal(2) neighbour of tid [l(2)not] and lethal(2) relative of tid." Kurzik-Dumke U., Kaymer M., Gundacker D., Debes A., Labitzke K. Gene 200:45-58(1997) [PubMed: 9373138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], CHARACTERIZATION. Strain: Apxo, bIf, Harvich and Oregon-R. Tissue: Embryo.
[2]	"Sequence of a 10291 nt genomic region of Drosophila melanogaster harbouring the genes l(2)tid, l(2)not, l(2)rot, and l(2)dtl." Gundacker S., Neubhuer M., Kurzik-Dumke U. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Oregon-2.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-510. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95241 Genomic DNA. Translation: CAA64529.1. X77820 mRNA. Translation: CAA54835.1. X95248 Genomic DNA. Translation: CAA64537.1. X95252 Genomic DNA. Translation: CAA64541.1. X95243 Genomic DNA. Translation: CAA64532.1. Y10074 Genomic DNA. Translation: CAA71167.1. X98094 Genomic DNA. Translation: CAA66721.1. AE013599 Genomic DNA. Translation: AAF47050.1. AY061582 mRNA. Translation: AAL29130.1. Different initiation.
PIR	S42090.
RefSeq	NP_523829.2. NM_079105.2.
UniGene	Dm.2569.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q27333. 1 interaction.
MINT	MINT-757739.
STRING	Q27333.
Protein family/group databases
CAZy	GT58. Glycosyltransferase Family 58.
Proteomic databases
PRIDE	Q27333.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	37754.
KEGG	dme:Dmel_CG4084.
Organism-specific databases
FlyBase	FBgn0011297. l(2)not.
Phylogenomic databases
eggNOG	inNOG07103.
InParanoid	Q27333.
PhylomeDB	Q27333.
Gene expression databases
Bgee	Q27333.
GermOnline	CG4084. Drosophila melanogaster.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
KO	K03845.
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	805248.

Entry information

Entry name

NT56_DROME

Accession

Primary (citable) accession number: Q27333
Secondary accession number(s): O02532 , O02533, Q24419, Q24420, Q24421, Q24607, Q95R76, Q9W1K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project