ID   NADA_APLKU              Reviewed;         282 AA.
AC   Q27312;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 42.
DE   RecName: Full=ADP-ribosyl cyclase;
DE            Short=ADRC;
DE            EC=3.2.2.5;
DE   AltName: Full=NAD(+) nucleosidase;
DE            Short=NADase;
DE   AltName: Full=NAD glycohydrolase;
DE   Flags: Precursor;
OS   Aplysia kurodai (Kuroda's sea hare).
OC   Eukaryota; Metazoa; Mollusca; Gastropoda; Orthogastropoda;
OC   Apogastropoda; Heterobranchia; Euthyneura; Opisthobranchia; Anaspidea;
OC   Aplysioidea; Aplysiidae; Aplysia.
OX   NCBI_TaxID=6501;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   TISSUE=Ovotestis;
RX   MEDLINE=95331619; PubMed=7607544; DOI=10.1016/0378-1119(95)00095-N;
RA   Nata K., Sugimoto T., Tohgo A., Takamura T., Noguchi N., Matsuoka A.,
RA   Numakunai T., Shikama K., Yonekura H., Takasawa S., Okamoto H.;
RT   "The structure of the Aplysia kurodai gene encoding ADP-ribosyl
RT   cyclase, a second-messenger enzyme.";
RL   Gene 158:213-218(1995).
RN   [2]
RP   MUTAGENESIS OF LYS-95 AND GLU-176.
RX   MEDLINE=95050652; PubMed=7961800;
RA   Tohgo A., Takasawa S., Noguchi N., Koguma T., Nata K., Sugimoto T.,
RA   Furuya Y., Yonekura H., Okamoto H.;
RT   "Essential cysteine residues for cyclic ADP-ribose synthesis and
RT   hydrolysis by CD38.";
RL   J. Biol. Chem. 269:28555-28557(1994).
CC   -!- FUNCTION: Synthesizes cyclic ADP-ribose, a second messenger for
CC       calcium mobilization from endoplasmic reticulum.
CC   -!- CATALYTIC ACTIVITY: NAD(+) + H(2)O = ADP-ribose + nicotinamide.
CC   -!- ENZYME REGULATION: Activity is presumably regulated by its
CC       sequestration in vesicles before egg fertilization. After
CC       fertilization and upon NADase release, it could then be regulated
CC       via its potential phosphorylation sites.
CC   -!- SUBCELLULAR LOCATION: Note=Localized to vesicles or granules
CC       within ova of all stages.
CC   -!- TISSUE SPECIFICITY: Ovotestis.
CC   -!- SIMILARITY: Belongs to the ADP-ribosyl cyclase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; D30048; BAA06284.1; -; mRNA.
DR   EMBL; D38536; BAA07537.1; -; Genomic_DNA.
DR   PIR; JC4134; JC4134.
DR   HSSP; P29241; 1LBE.
DR   SMR; Q27312; 25-275.
DR   BRENDA; 3.2.2.5; 290610.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003953; F:NAD+ nucleosidase activity; IEA:EC.
DR   GO; GO:0008152; P:metabolic process; IEA:InterPro.
DR   GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR   InterPro; IPR003193; ADP-ribosyl_cyclase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR10912; Rib_hydrolayse; 1.
DR   Pfam; PF02267; Rib_hydrolayse; 1.
PE   1: Evidence at protein level;
KW   Calcium; Disulfide bond; Fertilization; Hydrolase; NAD; Signal.
FT   SIGNAL        1     24       Potential.
FT   CHAIN        25    282       ADP-ribosyl cyclase.
FT                                /FTId=PRO_0000004031.
FT   DISULFID     39     58       By similarity.
FT   DISULFID     75    155       By similarity.
FT   DISULFID    136    149       By similarity.
FT   DISULFID    230    251       By similarity.
FT   DISULFID    263    272       By similarity.
FT   MUTAGEN      95     95       K->C: Has also cADPR hydrolase activity;
FT                                when associated with C-176.
FT   MUTAGEN     176    176       E->C: Has also cADPR hydrolase activity;
FT                                when associated with C-95.
SQ   SEQUENCE   282 AA;  31889 MW;  9725E7B00A16F97E CRC64;
     MSPVAIVACV CLAVTLTRIS PSEAIFPTPE LQNVFLGRCK DYEITRYLTI LPRVKSDCRA
     LWTNFFKAFS FKAPCNLDLG SYKDFFQRAQ QTLPKNKVMF WSGVYDEAHD FADDGRKYIT
     LEDTLPGYML NSLVWCGQRD KPGFNQKVCP DFKDCPVQAR ESFWGTASSS YAHSAEGDVT
     YMVDGSNPKV PAYRPDSFFG KYELPNLTNK VTKVKVIVLH QLGQKIIERC GAGSLLDLEM
     VVKAKKFGFD CVENPKSVLF LLCADNPNAR ECQLAKRYYR IA
//