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Q27297

- RAD51_DROME

UniProt

Q27297 - RAD51_DROME

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Protein

DNA repair protein Rad51 homolog

Gene
spn-A, DMR, Rad51, CG7948
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA By similarity.2 Publications
Spindle genes are required for each of the symmetry-breaking steps that generate polarity during egg axis formation; oocyte positioning at the posterior of the cyst to generate the first AP polarity and inhibition of gurken (grk) signaling to the follicle cell layer to polarize first the AP axis and then DV axis. May have a role in female meiosis.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 1318ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. damaged DNA binding Source: InterPro
  3. DNA-dependent ATPase activity Source: InterPro
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: FlyBase
  2. double-strand break repair via synthesis-dependent strand annealing Source: FlyBase
  3. female meiotic division Source: FlyBase
  4. germarium-derived oocyte fate determination Source: FlyBase
  5. intracellular mRNA localization Source: FlyBase
  6. karyosome formation Source: FlyBase
  7. oocyte differentiation Source: FlyBase
  8. oogenesis Source: FlyBase
  9. polarity specification of anterior/posterior axis Source: FlyBase
  10. polarity specification of dorsal/ventral axis Source: FlyBase
  11. regulation of double-strand break repair via homologous recombination Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_210703. Assembly of the RAD51-ssDNA nucleoprotein complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein Rad51 homolog
Alternative name(s):
Protein spindle-A
RecA protein homolog
Gene namesi
Name:spn-A
Synonyms:DMR, Rad51
ORF Names:CG7948
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003479. spn-A.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336DNA repair protein Rad51 homologPRO_0000122938Add
BLAST

Proteomic databases

PaxDbiQ27297.
PRIDEiQ27297.

Expressioni

Tissue specificityi

Highly expressed in ovaries.1 Publication

Gene expression databases

BgeeiQ27297.

Interactioni

Protein-protein interaction databases

BioGridi68433. 69 interactions.
DIPiDIP-20846N.
IntActiQ27297. 37 interactions.
MINTiMINT-337614.

Structurei

3D structure databases

ProteinModelPortaliQ27297.
SMRiQ27297. Positions 22-335.

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.

Phylogenomic databases

eggNOGiCOG0468.
GeneTreeiENSGT00750000117750.
InParanoidiQ27297.
KOiK04482.
OMAiMMAESRY.
OrthoDBiEOG715Q4H.
PhylomeDBiQ27297.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q27297-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKLTNVQAQ QEEEEEEGPL SVTKLIGGSI TAKDIKLLQQ ASLHTVESVA    50
NATKKQLMAI PGLGGGKVEQ IITEANKLVP LGFLSARTFY QMRADVVQLS 100
TGSKELDKLL GGGIETGSIT EIFGEFRCGK TQLCHTLAVT CQLPISQKGG 150
EGKCMYIDTE NTFRPERLAA IAQRYKLNES EVLDNVAFTR AHNSDQQTKL 200
IQMAAGMLFE SRYALLIVDS AMALYRSDYI GRGELAARQN HLGLFLRMLQ 250
RLADEFGVAV VITNQVTASL DGAPGMFDAK KPIGGHIMAH SSTTRLYLRK 300
GKGETRICKI YDSPCLPESE AMFAILPDGI GDARES 336
Length:336
Mass (Da):36,647
Last modified:November 1, 1997 - v1
Checksum:iF9E9B21405B15DB0
GO
Isoform B (identifier: Q27297-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.

Note: No experimental confirmation available.

Show »
Length:279
Mass (Da):30,459
Checksum:iBF82A5C319AB61F9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5757Missing in isoform B. VSP_012414Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti334 – 3363RES → GRANCAHL in AAN71546. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37788 Genomic DNA. Translation: BAA07039.1.
D17726 mRNA. Translation: BAA04580.1.
L41342 Genomic DNA. Translation: AAA64873.1.
AE014297 Genomic DNA. Translation: AAF57005.1.
AE014297 Genomic DNA. Translation: AAN14213.1.
BT001791 mRNA. Translation: AAN71546.1.
RefSeqiNP_524583.1. NM_079844.4. [Q27297-1]
NP_733342.1. NM_170463.3. [Q27297-2]
UniGeneiDm.1913.

Genome annotation databases

EnsemblMetazoaiFBtr0085589; FBpp0084955; FBgn0003479. [Q27297-1]
GeneIDi43577.
KEGGidme:Dmel_CG7948.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37788 Genomic DNA. Translation: BAA07039.1 .
D17726 mRNA. Translation: BAA04580.1 .
L41342 Genomic DNA. Translation: AAA64873.1 .
AE014297 Genomic DNA. Translation: AAF57005.1 .
AE014297 Genomic DNA. Translation: AAN14213.1 .
BT001791 mRNA. Translation: AAN71546.1 .
RefSeqi NP_524583.1. NM_079844.4. [Q27297-1 ]
NP_733342.1. NM_170463.3. [Q27297-2 ]
UniGenei Dm.1913.

3D structure databases

ProteinModelPortali Q27297.
SMRi Q27297. Positions 22-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 68433. 69 interactions.
DIPi DIP-20846N.
IntActi Q27297. 37 interactions.
MINTi MINT-337614.

Proteomic databases

PaxDbi Q27297.
PRIDEi Q27297.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0085589 ; FBpp0084955 ; FBgn0003479 . [Q27297-1 ]
GeneIDi 43577.
KEGGi dme:Dmel_CG7948.

Organism-specific databases

CTDi 43577.
FlyBasei FBgn0003479. spn-A.

Phylogenomic databases

eggNOGi COG0468.
GeneTreei ENSGT00750000117750.
InParanoidi Q27297.
KOi K04482.
OMAi MMAESRY.
OrthoDBi EOG715Q4H.
PhylomeDBi Q27297.

Enzyme and pathway databases

Reactomei REACT_210703. Assembly of the RAD51-ssDNA nucleoprotein complex.

Miscellaneous databases

GenomeRNAii 43577.
NextBioi 834653.
PROi Q27297.

Gene expression databases

Bgeei Q27297.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view ]
Pfami PF08423. Rad51. 1 hit.
[Graphical view ]
PIRSFi PIRSF005856. Rad51. 1 hit.
SMARTi SM00382. AAA. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR02239. recomb_RAD51. 1 hit.
PROSITEi PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the cDNA and genomic DNA that correspond to the recA-like gene of Drosophila melanogaster."
    Akaboshi E., Inoue Y., Ryo H.
    Jpn. J. Genet. 69:663-670(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A).
    Strain: Canton-S.
  2. "A recA-like gene in Drosophila melanogaster that is expressed at high levels in female but not male meiotic tissues."
    McKee B.D., Ren X.J., Hong C.S.
    Chromosoma 104:479-488(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY.
    Strain: Oregon-R.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Head.
  6. "Oocyte determination and the origin of polarity in Drosophila: the role of the spindle genes."
    Gonzalez-Reyes A., Elliott H., St Johnston D.
    Development 124:4927-4937(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRAD51_DROME
AccessioniPrimary (citable) accession number: Q27297
Secondary accession number(s): Q8IGG8, Q8IMJ5, Q9VAA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi