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Q27297

- RAD51_DROME

UniProt

Q27297 - RAD51_DROME

Protein

DNA repair protein Rad51 homolog

Gene

spn-A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA By similarity.By similarity
    Spindle genes are required for each of the symmetry-breaking steps that generate polarity during egg axis formation; oocyte positioning at the posterior of the cyst to generate the first AP polarity and inhibition of gurken (grk) signaling to the follicle cell layer to polarize first the AP axis and then DV axis. May have a role in female meiosis.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi124 – 1318ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. damaged DNA binding Source: InterPro
    3. DNA-dependent ATPase activity Source: InterPro
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. DNA repair Source: FlyBase
    2. double-strand break repair via synthesis-dependent strand annealing Source: FlyBase
    3. female meiotic division Source: FlyBase
    4. germarium-derived oocyte fate determination Source: FlyBase
    5. intracellular mRNA localization Source: FlyBase
    6. karyosome formation Source: FlyBase
    7. oocyte differentiation Source: FlyBase
    8. oogenesis Source: FlyBase
    9. polarity specification of anterior/posterior axis Source: FlyBase
    10. polarity specification of dorsal/ventral axis Source: FlyBase
    11. regulation of double-strand break repair via homologous recombination Source: FlyBase

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_210703. Assembly of the RAD51-ssDNA nucleoprotein complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein Rad51 homolog
    Alternative name(s):
    Protein spindle-A
    RecA protein homolog
    Gene namesi
    Name:spn-A
    Synonyms:DMR, Rad51
    ORF Names:CG7948
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0003479. spn-A.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336DNA repair protein Rad51 homologPRO_0000122938Add
    BLAST

    Proteomic databases

    PaxDbiQ27297.
    PRIDEiQ27297.

    Expressioni

    Tissue specificityi

    Highly expressed in ovaries.1 Publication

    Gene expression databases

    BgeeiQ27297.

    Interactioni

    Protein-protein interaction databases

    BioGridi68433. 69 interactions.
    DIPiDIP-20846N.
    IntActiQ27297. 37 interactions.
    MINTiMINT-337614.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27297.
    SMRiQ27297. Positions 22-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RecA family. RAD51 subfamily.Curated
    Contains 1 HhH domain.Curated

    Phylogenomic databases

    eggNOGiCOG0468.
    GeneTreeiENSGT00750000117750.
    InParanoidiQ27297.
    KOiK04482.
    OMAiMMAESRY.
    OrthoDBiEOG715Q4H.
    PhylomeDBiQ27297.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR011941. DNA_recomb/repair_Rad51.
    IPR013632. DNA_recomb/repair_Rad51_C.
    IPR016467. DNA_recomb/repair_RecA-like.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view]
    PfamiPF08423. Rad51. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005856. Rad51. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47794. SSF47794. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
    PROSITEiPS50162. RECA_2. 1 hit.
    PS50163. RECA_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q27297-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKLTNVQAQ QEEEEEEGPL SVTKLIGGSI TAKDIKLLQQ ASLHTVESVA    50
    NATKKQLMAI PGLGGGKVEQ IITEANKLVP LGFLSARTFY QMRADVVQLS 100
    TGSKELDKLL GGGIETGSIT EIFGEFRCGK TQLCHTLAVT CQLPISQKGG 150
    EGKCMYIDTE NTFRPERLAA IAQRYKLNES EVLDNVAFTR AHNSDQQTKL 200
    IQMAAGMLFE SRYALLIVDS AMALYRSDYI GRGELAARQN HLGLFLRMLQ 250
    RLADEFGVAV VITNQVTASL DGAPGMFDAK KPIGGHIMAH SSTTRLYLRK 300
    GKGETRICKI YDSPCLPESE AMFAILPDGI GDARES 336
    Length:336
    Mass (Da):36,647
    Last modified:November 1, 1997 - v1
    Checksum:iF9E9B21405B15DB0
    GO
    Isoform B (identifier: Q27297-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-57: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:279
    Mass (Da):30,459
    Checksum:iBF82A5C319AB61F9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti334 – 3363RES → GRANCAHL in AAN71546. (PubMed:12537569)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5757Missing in isoform B. 1 PublicationVSP_012414Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37788 Genomic DNA. Translation: BAA07039.1.
    D17726 mRNA. Translation: BAA04580.1.
    L41342 Genomic DNA. Translation: AAA64873.1.
    AE014297 Genomic DNA. Translation: AAF57005.1.
    AE014297 Genomic DNA. Translation: AAN14213.1.
    BT001791 mRNA. Translation: AAN71546.1.
    RefSeqiNP_524583.1. NM_079844.4. [Q27297-1]
    NP_733342.1. NM_170463.3. [Q27297-2]
    UniGeneiDm.1913.

    Genome annotation databases

    EnsemblMetazoaiFBtr0085589; FBpp0084955; FBgn0003479. [Q27297-1]
    GeneIDi43577.
    KEGGidme:Dmel_CG7948.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37788 Genomic DNA. Translation: BAA07039.1 .
    D17726 mRNA. Translation: BAA04580.1 .
    L41342 Genomic DNA. Translation: AAA64873.1 .
    AE014297 Genomic DNA. Translation: AAF57005.1 .
    AE014297 Genomic DNA. Translation: AAN14213.1 .
    BT001791 mRNA. Translation: AAN71546.1 .
    RefSeqi NP_524583.1. NM_079844.4. [Q27297-1 ]
    NP_733342.1. NM_170463.3. [Q27297-2 ]
    UniGenei Dm.1913.

    3D structure databases

    ProteinModelPortali Q27297.
    SMRi Q27297. Positions 22-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 68433. 69 interactions.
    DIPi DIP-20846N.
    IntActi Q27297. 37 interactions.
    MINTi MINT-337614.

    Proteomic databases

    PaxDbi Q27297.
    PRIDEi Q27297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0085589 ; FBpp0084955 ; FBgn0003479 . [Q27297-1 ]
    GeneIDi 43577.
    KEGGi dme:Dmel_CG7948.

    Organism-specific databases

    CTDi 43577.
    FlyBasei FBgn0003479. spn-A.

    Phylogenomic databases

    eggNOGi COG0468.
    GeneTreei ENSGT00750000117750.
    InParanoidi Q27297.
    KOi K04482.
    OMAi MMAESRY.
    OrthoDBi EOG715Q4H.
    PhylomeDBi Q27297.

    Enzyme and pathway databases

    Reactomei REACT_210703. Assembly of the RAD51-ssDNA nucleoprotein complex.

    Miscellaneous databases

    GenomeRNAii 43577.
    NextBioi 834653.
    PROi Q27297.

    Gene expression databases

    Bgeei Q27297.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR011941. DNA_recomb/repair_Rad51.
    IPR013632. DNA_recomb/repair_Rad51_C.
    IPR016467. DNA_recomb/repair_RecA-like.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view ]
    Pfami PF08423. Rad51. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005856. Rad51. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47794. SSF47794. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR02239. recomb_RAD51. 1 hit.
    PROSITEi PS50162. RECA_2. 1 hit.
    PS50163. RECA_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the cDNA and genomic DNA that correspond to the recA-like gene of Drosophila melanogaster."
      Akaboshi E., Inoue Y., Ryo H.
      Jpn. J. Genet. 69:663-670(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A).
      Strain: Canton-S.
    2. "A recA-like gene in Drosophila melanogaster that is expressed at high levels in female but not male meiotic tissues."
      McKee B.D., Ren X.J., Hong C.S.
      Chromosoma 104:479-488(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY.
      Strain: Oregon-R.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Head.
    6. "Oocyte determination and the origin of polarity in Drosophila: the role of the spindle genes."
      Gonzalez-Reyes A., Elliott H., St Johnston D.
      Development 124:4927-4937(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRAD51_DROME
    AccessioniPrimary (citable) accession number: Q27297
    Secondary accession number(s): Q8IGG8, Q8IMJ5, Q9VAA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3