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Protein

DNA repair protein Rad51 homolog

Gene

spn-A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA (By similarity).By similarity
Spindle genes are required for each of the symmetry-breaking steps that generate polarity during egg axis formation; oocyte positioning at the posterior of the cyst to generate the first AP polarity and inhibition of gurken (grk) signaling to the follicle cell layer to polarize first the AP axis and then DV axis. May have a role in female meiosis.2 Publications

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 131ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA recombinase assembly Source: GO_Central
  • DNA recombination Source: FlyBase
  • DNA repair Source: FlyBase
  • double-strand break repair Source: FlyBase
  • double-strand break repair via synthesis-dependent strand annealing Source: FlyBase
  • female meiotic nuclear division Source: FlyBase
  • germarium-derived oocyte fate determination Source: FlyBase
  • intracellular mRNA localization Source: FlyBase
  • mitotic recombination Source: GO_Central
  • mitotic recombination-dependent replication fork processing Source: InterPro
  • oocyte fate determination Source: FlyBase
  • oocyte karyosome formation Source: FlyBase
  • oogenesis Source: FlyBase
  • polarity specification of anterior/posterior axis Source: FlyBase
  • polarity specification of dorsal/ventral axis Source: FlyBase
  • reciprocal meiotic recombination Source: GO_Central
  • regulation of double-strand break repair via homologous recombination Source: FlyBase
  • response to caffeine Source: FlyBase
  • response to ionizing radiation Source: GO_Central
  • strand invasion Source: GO_Central

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processMeiosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-5693616 Presynaptic phase of homologous DNA pairing and strand exchange

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein Rad51 homolog
Alternative name(s):
Protein spindle-A
RecA protein homolog
Gene namesi
Name:spn-A
Synonyms:DMR, Rad51
ORF Names:CG7948
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003479 spn-A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229381 – 336DNA repair protein Rad51 homologAdd BLAST336

Proteomic databases

PaxDbiQ27297
PRIDEiQ27297

Expressioni

Tissue specificityi

Highly expressed in ovaries.1 Publication

Gene expression databases

BgeeiFBgn0003479
ExpressionAtlasiQ27297 differential
GenevisibleiQ27297 DM

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi6843381 interactors.
DIPiDIP-20846N
IntActiQ27297 70 interactors.
STRINGi7227.FBpp0084955

Structurei

3D structure databases

ProteinModelPortaliQ27297
SMRiQ27297
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1433 Eukaryota
COG0468 LUCA
GeneTreeiENSGT00890000139508
InParanoidiQ27297
KOiK04482
OMAiYNTDHQT
OrthoDBiEOG091G09QY
PhylomeDBiQ27297

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011941 DNA_recomb/repair_Rad51
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR010995 DNA_repair_Rad51/TF_NusA_a-hlx
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
IPR020587 RecA_monomer-monomer_interface
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF47794 SSF47794, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02239 recomb_RAD51, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q27297-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKLTNVQAQ QEEEEEEGPL SVTKLIGGSI TAKDIKLLQQ ASLHTVESVA
60 70 80 90 100
NATKKQLMAI PGLGGGKVEQ IITEANKLVP LGFLSARTFY QMRADVVQLS
110 120 130 140 150
TGSKELDKLL GGGIETGSIT EIFGEFRCGK TQLCHTLAVT CQLPISQKGG
160 170 180 190 200
EGKCMYIDTE NTFRPERLAA IAQRYKLNES EVLDNVAFTR AHNSDQQTKL
210 220 230 240 250
IQMAAGMLFE SRYALLIVDS AMALYRSDYI GRGELAARQN HLGLFLRMLQ
260 270 280 290 300
RLADEFGVAV VITNQVTASL DGAPGMFDAK KPIGGHIMAH SSTTRLYLRK
310 320 330
GKGETRICKI YDSPCLPESE AMFAILPDGI GDARES
Length:336
Mass (Da):36,647
Last modified:November 1, 1997 - v1
Checksum:iF9E9B21405B15DB0
GO
Isoform B (identifier: Q27297-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.

Note: No experimental confirmation available.
Show »
Length:279
Mass (Da):30,459
Checksum:iBF82A5C319AB61F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti334 – 336RES → GRANCAHL in AAN71546 (PubMed:12537569).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0124141 – 57Missing in isoform B. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37788 Genomic DNA Translation: BAA07039.1
D17726 mRNA Translation: BAA04580.1
L41342 Genomic DNA Translation: AAA64873.1
AE014297 Genomic DNA Translation: AAF57005.1
AE014297 Genomic DNA Translation: AAN14213.1
BT001791 mRNA Translation: AAN71546.1
RefSeqiNP_524583.1, NM_079844.4 [Q27297-1]
NP_733342.1, NM_170463.3 [Q27297-2]
UniGeneiDm.1913

Genome annotation databases

EnsemblMetazoaiFBtr0085589; FBpp0084955; FBgn0003479 [Q27297-1]
FBtr0085590; FBpp0084956; FBgn0003479 [Q27297-2]
GeneIDi43577
KEGGidme:Dmel_CG7948

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRAD51_DROME
AccessioniPrimary (citable) accession number: Q27297
Secondary accession number(s): Q8IGG8, Q8IMJ5, Q9VAA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome