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Protein

ATP-dependent RNA helicase WM6

Gene

Hel25E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for mRNA export out of the nucleus. Probable RNA helicase that may regulate entry into mitosis by down-regulating the expression of other genes whose activity may be rate-limiting for entry into mitosis during embryogenesis. Binds to salivary gland chromosomes and modifies position effect variegation. Promotes an open chromatin structure that favors transcription during development by regulating the spread of heterochromatin.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: FlyBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: GO_Central
  • chromatin remodeling Source: FlyBase
  • mRNA export from nucleus Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-109688 Cleavage of Growing Transcript in the Termination Region
R-DME-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-DME-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase WM6 (EC:3.6.4.13)
Short name:
DEAD box protein UAP56
Short name:
Dmrnahel
Alternative name(s):
HEL/UAP56
Gene namesi
Name:Hel25E
Synonyms:Dbp25F, hel, WM6
ORF Names:CG7269
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0014189 Hel25E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550801 – 424ATP-dependent RNA helicase WM6Add BLAST424

Proteomic databases

PaxDbiQ27268
PRIDEiQ27268

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0014189
ExpressionAtlasiQ27268 baseline and differential
GenevisibleiQ27268 DM

Interactioni

Subunit structurei

Component of the spliceosome (Probable). Interacts with the exon junction complex.Curated1 Publication

Protein-protein interaction databases

BioGridi59949, 48 interactors
DIPiDIP-20199N
IntActiQ27268, 4 interactors
STRINGi7227.FBpp0078754

Structurei

3D structure databases

ProteinModelPortaliQ27268
SMRiQ27268
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 246Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini258 – 419Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 69Q motifAdd BLAST29
Motifi193 – 196DECD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0329 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00830000128348
InParanoidiQ27268
KOiK12812
OMAiCRKFMQN
OrthoDBiEOG091G0H5W
PhylomeDBiQ27268

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q27268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNDDLLDY EDEEQTETTA VENQEAPKKD VKGTYVSIHS SGFRDFLLKP
60 70 80 90 100
EILRAIVDCG FEHPSEVQHE CIPQAVLGMD ILCQAKSGMG KTAVFVLATL
110 120 130 140 150
QQLEPSDNNT CHVLVMCHTR ELAFQISKEY ERFSKYMPTV KVAVFFGGMA
160 170 180 190 200
IQKDEETLKS GTPHIVVGTP GRILALIRNK KLNLKLLKHF VLDECDKMLE
210 220 230 240 250
QLDMRRDVQE IFRSTPHGKQ VMMFSATLSK DIRPVCKKFM QDPMEVYVDD
260 270 280 290 300
EAKLTLHGLQ QHYVNLKENE KNKKLFELLD VLEFNQVVIF VKSVQRCVAL
310 320 330 340 350
SQLLTEQNFP AIGIHRGMTQ EERLNRYQQF KDFQKRILVA TNLFGRGMDI
360 370 380 390 400
ERVNIVFNYD MPEDSDTYLH RVARAGRFGT KGLAITFVSD ENDAKILNEV
410 420
QDRFDVNISE LPEEIDLSTY IEGR
Length:424
Mass (Da):48,652
Last modified:November 1, 1996 - v1
Checksum:iCE132476090E1AFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79802 mRNA Translation: CAA56197.1
L06018 Genomic DNA Translation: AAB65835.1
AE014134 Genomic DNA Translation: AAF52261.1
AE014134 Genomic DNA Translation: AAN10544.1
AE014134 Genomic DNA Translation: AAN10545.1
AY118921 mRNA Translation: AAM50781.1
PIRiS51601
RefSeqiNP_723089.1, NM_164646.3
NP_723090.1, NM_164647.1
NP_723091.1, NM_164648.2
UniGeneiDm.3332

Genome annotation databases

EnsemblMetazoaiFBtr0079123; FBpp0078754; FBgn0014189
FBtr0079124; FBpp0078755; FBgn0014189
FBtr0079125; FBpp0078756; FBgn0014189
GeneIDi33781
KEGGidme:Dmel_CG7269

Similar proteinsi

Entry informationi

Entry nameiDX39B_DROME
AccessioniPrimary (citable) accession number: Q27268
Secondary accession number(s): Q540X0, Q9VMQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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