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Protein

ATP-dependent RNA helicase WM6

Gene

Hel25E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mRNA export out of the nucleus. Probable RNA helicase that may regulate entry into mitosis by down-regulating the expression of other genes whose activity may be rate-limiting for entry into mitosis during embryogenesis. Binds to salivary gland chromosomes and modifies position effect variegation. Promotes an open chromatin structure that favors transcription during development by regulating the spread of heterochromatin.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: FlyBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: GO_Central
  • chromatin remodeling Source: FlyBase
  • mRNA export from nucleus Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-109688. Cleavage of Growing Transcript in the Termination Region.
R-DME-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-DME-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase WM6 (EC:3.6.4.13)
Short name:
DEAD box protein UAP56
Short name:
Dmrnahel
Alternative name(s):
HEL/UAP56
Gene namesi
Name:Hel25E
Synonyms:Dbp25F, hel, WM6
ORF Names:CG7269
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0014189. Hel25E.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550801 – 424ATP-dependent RNA helicase WM6Add BLAST424

Proteomic databases

PaxDbiQ27268.
PRIDEiQ27268.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0014189.
ExpressionAtlasiQ27268. differential.
GenevisibleiQ27268. DM.

Interactioni

Subunit structurei

Component of the spliceosome (Probable). Interacts with the exon junction complex.Curated1 Publication

Protein-protein interaction databases

BioGridi59949. 48 interactors.
DIPiDIP-20199N.
IntActiQ27268. 4 interactors.
MINTiMINT-304772.
STRINGi7227.FBpp0078754.

Structurei

3D structure databases

ProteinModelPortaliQ27268.
SMRiQ27268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 246Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini258 – 419Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 69Q motifAdd BLAST29
Motifi193 – 196DECD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0329. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00830000128348.
InParanoidiQ27268.
KOiK12812.
OMAiIICQAKS.
OrthoDBiEOG091G0H5W.
PhylomeDBiQ27268.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNDDLLDY EDEEQTETTA VENQEAPKKD VKGTYVSIHS SGFRDFLLKP
60 70 80 90 100
EILRAIVDCG FEHPSEVQHE CIPQAVLGMD ILCQAKSGMG KTAVFVLATL
110 120 130 140 150
QQLEPSDNNT CHVLVMCHTR ELAFQISKEY ERFSKYMPTV KVAVFFGGMA
160 170 180 190 200
IQKDEETLKS GTPHIVVGTP GRILALIRNK KLNLKLLKHF VLDECDKMLE
210 220 230 240 250
QLDMRRDVQE IFRSTPHGKQ VMMFSATLSK DIRPVCKKFM QDPMEVYVDD
260 270 280 290 300
EAKLTLHGLQ QHYVNLKENE KNKKLFELLD VLEFNQVVIF VKSVQRCVAL
310 320 330 340 350
SQLLTEQNFP AIGIHRGMTQ EERLNRYQQF KDFQKRILVA TNLFGRGMDI
360 370 380 390 400
ERVNIVFNYD MPEDSDTYLH RVARAGRFGT KGLAITFVSD ENDAKILNEV
410 420
QDRFDVNISE LPEEIDLSTY IEGR
Length:424
Mass (Da):48,652
Last modified:November 1, 1996 - v1
Checksum:iCE132476090E1AFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79802 mRNA. Translation: CAA56197.1.
L06018 Genomic DNA. Translation: AAB65835.1.
AE014134 Genomic DNA. Translation: AAF52261.1.
AE014134 Genomic DNA. Translation: AAN10544.1.
AE014134 Genomic DNA. Translation: AAN10545.1.
AY118921 mRNA. Translation: AAM50781.1.
PIRiS51601.
RefSeqiNP_723089.1. NM_164646.3.
NP_723090.1. NM_164647.1.
NP_723091.1. NM_164648.2.
UniGeneiDm.3332.

Genome annotation databases

EnsemblMetazoaiFBtr0079123; FBpp0078754; FBgn0014189.
FBtr0079124; FBpp0078755; FBgn0014189.
FBtr0079125; FBpp0078756; FBgn0014189.
GeneIDi33781.
KEGGidme:Dmel_CG7269.

Similar proteinsi

Entry informationi

Entry nameiDX39B_DROME
AccessioniPrimary (citable) accession number: Q27268
Secondary accession number(s): Q540X0, Q9VMQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families