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Q27182

- T4A_PARTE

UniProt

Q27182 - T4A_PARTE

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Protein

Trichocyst matrix protein T4-A

Gene
T4A, GSPATT00004255001
Organism
Paramecium tetraurelia
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that crystallize inside the trichocyst matrix.

Names & Taxonomyi

Protein namesi
Recommended name:
Trichocyst matrix protein T4-A
Alternative name(s):
Secretory granule protein T4-A
TMP 4-A
Cleaved into the following 2 chains:
Gene namesi
Name:T4A
ORF Names:GSPATT00004255001
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
ProteomesiUP000000600: Partially assembled WGS sequence

Subcellular locationi

Trichocyst
Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

GO - Cellular componenti

  1. trichocyst Source: UniProtKB-SubCell
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed predictionAdd
BLAST
Propeptidei18 – 5235PRO_0000034385Add
BLAST
Chaini53 – 189137Trichocyst matrix protein T4-A 1PRO_0000034386Add
BLAST
Propeptidei190 – 22132PRO_0000034387Add
BLAST
Chaini222 – 363142Trichocyst matrix protein T4-A 2PRO_0000034388Add
BLAST

Post-translational modificationi

Two components are produced by post-translational processing from the precursor peptide.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili85 – 11935 Reviewed predictionAdd
BLAST
Coiled coili244 – 352109 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the TMP family.

Keywords - Domaini

Coiled coil, Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27182-1 [UniParc]FASTAAdd to Basket

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MARSLTILAI VFAVATARVT KSESPKEILA QVNKDSFGNS ILSVLQLQLA    50
TGGPVGEIQI LLNNIASQLN GDQKKADKVH ESDTVAFEKI IADLEQEIAY 100
HQTQIVALSN LRDSTTEALG EAEVEVRVVT SDIANNEKSF ADESATRQSQ 150
HDTWVRKDAE HVDQMEAIDE ASKIVQHLQA GVAFAQLKSR FEKVQAKLME 200
SKHALFKPLI NALTQLASKV DNKSIIKILE LLAQIRQQLV ASRASLLATE 250
ERQAANWEVQ SSHLQEEHKR LVERKAFLEN SIVQFKVTIQ EAVEDLEDQT 300
LFLEDAEDSL AIQERWAAEQ ESQYEAQTFE REQQLEVVER LQEVLTQKLS 350
AASEFLQVRE EVF 363
Length:363
Mass (Da):40,769
Last modified:October 23, 2007 - v2
Checksum:i7CECC42F37A1D705
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 802VH → LD in AAC47029. 1 Publication
Sequence conflicti343 – 3431E → Q in AAC47029. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U47117 Genomic DNA. Translation: AAC47029.1.
CT867986 Genomic DNA. Translation: CAK56161.1.
RefSeqiXP_001423559.1. XM_001423522.1.
UniGeneiPte.18507.

Genome annotation databases

EnsemblProtistsiCAK56161; CAK56161; GSPATT00004255001.
GeneIDi5009343.
KEGGiptm:GSPATT00004255001.

Cross-referencesi

Web resourcesi

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U47117 Genomic DNA. Translation: AAC47029.1 .
CT867986 Genomic DNA. Translation: CAK56161.1 .
RefSeqi XP_001423559.1. XM_001423522.1.
UniGenei Pte.18507.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi CAK56161 ; CAK56161 ; GSPATT00004255001 .
GeneIDi 5009343.
KEGGi ptm:GSPATT00004255001.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
    Gautier M.-C., Sperling L., Madeddu L.
    J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Stock d4-2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Stock d4-2.
  3. "A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
    Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
    Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-79.
    Strain: Stock d4-2.
  4. "Protein processing and morphogenesis of secretory granules in Paramecium."
    Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
    Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: Stock d4-2.

Entry informationi

Entry nameiT4A_PARTE
AccessioniPrimary (citable) accession number: Q27182
Secondary accession number(s): A0BC94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 23, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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