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Q27182 (T4A_PARTE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trichocyst matrix protein T4-A
Alternative name(s):
Secretory granule protein T4-A
TMP 4-A
Gene names
Name:T4A
ORF Names:GSPATT00004255001
OrganismParamecium tetraurelia [Reference proteome]
Taxonomic identifier5888 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Structural protein that crystallize inside the trichocyst matrix.

Subcellular location

Trichocyst. Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

Post-translational modification

Two components are produced by post-translational processing from the precursor peptide.

Sequence similarities

Belongs to the TMP family.

Ontologies

Keywords
   DomainCoiled coil
Signal
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componenttrichocyst

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 5235
PRO_0000034385
Chain53 – 189137Trichocyst matrix protein T4-A 1
PRO_0000034386
Propeptide190 – 22132
PRO_0000034387
Chain222 – 363142Trichocyst matrix protein T4-A 2
PRO_0000034388

Regions

Coiled coil85 – 11935 Potential
Coiled coil244 – 352109 Potential

Experimental info

Sequence conflict79 – 802VH → LD in AAC47029. Ref.1
Sequence conflict3431E → Q in AAC47029. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q27182 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: 7CECC42F37A1D705

FASTA36340,769
        10         20         30         40         50         60 
MARSLTILAI VFAVATARVT KSESPKEILA QVNKDSFGNS ILSVLQLQLA TGGPVGEIQI 

        70         80         90        100        110        120 
LLNNIASQLN GDQKKADKVH ESDTVAFEKI IADLEQEIAY HQTQIVALSN LRDSTTEALG 

       130        140        150        160        170        180 
EAEVEVRVVT SDIANNEKSF ADESATRQSQ HDTWVRKDAE HVDQMEAIDE ASKIVQHLQA 

       190        200        210        220        230        240 
GVAFAQLKSR FEKVQAKLME SKHALFKPLI NALTQLASKV DNKSIIKILE LLAQIRQQLV 

       250        260        270        280        290        300 
ASRASLLATE ERQAANWEVQ SSHLQEEHKR LVERKAFLEN SIVQFKVTIQ EAVEDLEDQT 

       310        320        330        340        350        360 
LFLEDAEDSL AIQERWAAEQ ESQYEAQTFE REQQLEVVER LQEVLTQKLS AASEFLQVRE 


EVF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
Gautier M.-C., Sperling L., Madeddu L.
J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Stock d4-2.
[2]"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia."
Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. expand/collapse author list , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P.
Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Stock d4-2.
[3]"A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-79.
Strain: Stock d4-2.
[4]"Protein processing and morphogenesis of secretory granules in Paramecium."
Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Strain: Stock d4-2.

Web resources

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47117 Genomic DNA. Translation: AAC47029.1.
CT867986 Genomic DNA. Translation: CAK56161.1.
RefSeqXP_001423559.1. XM_001423522.1.
UniGenePte.18507.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsCAK56161; CAK56161; GSPATT00004255001.
GeneID5009343.
KEGGptm:GSPATT00004255001.

Phylogenomic databases

ProtClustDBCLSZ2447240.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT4A_PARTE
AccessionPrimary (citable) accession number: Q27182
Secondary accession number(s): A0BC94
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 23, 2007
Last modified: February 19, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries