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Q27182

- T4A_PARTE

UniProt

Q27182 - T4A_PARTE

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Protein

Trichocyst matrix protein T4-A

Gene

T4A

Organism
Paramecium tetraurelia
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Structural protein that crystallize inside the trichocyst matrix.

Names & Taxonomyi

Protein namesi
Recommended name:
Trichocyst matrix protein T4-A
Alternative name(s):
Secretory granule protein T4-A
TMP 4-A
Cleaved into the following 2 chains:
Gene namesi
Name:T4A
ORF Names:GSPATT00004255001
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
ProteomesiUP000000600: Partially assembled WGS sequence

Subcellular locationi

Trichocyst
Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Propeptidei18 – 5235PRO_0000034385Add
BLAST
Chaini53 – 189137Trichocyst matrix protein T4-A 1PRO_0000034386Add
BLAST
Propeptidei190 – 22132PRO_0000034387Add
BLAST
Chaini222 – 363142Trichocyst matrix protein T4-A 2PRO_0000034388Add
BLAST

Post-translational modificationi

Two components are produced by post-translational processing from the precursor peptide.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili85 – 11935Sequence AnalysisAdd
BLAST
Coiled coili244 – 352109Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TMP family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

InParanoidiQ27182.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q27182-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MARSLTILAI VFAVATARVT KSESPKEILA QVNKDSFGNS ILSVLQLQLA
60 70 80 90 100
TGGPVGEIQI LLNNIASQLN GDQKKADKVH ESDTVAFEKI IADLEQEIAY
110 120 130 140 150
HQTQIVALSN LRDSTTEALG EAEVEVRVVT SDIANNEKSF ADESATRQSQ
160 170 180 190 200
HDTWVRKDAE HVDQMEAIDE ASKIVQHLQA GVAFAQLKSR FEKVQAKLME
210 220 230 240 250
SKHALFKPLI NALTQLASKV DNKSIIKILE LLAQIRQQLV ASRASLLATE
260 270 280 290 300
ERQAANWEVQ SSHLQEEHKR LVERKAFLEN SIVQFKVTIQ EAVEDLEDQT
310 320 330 340 350
LFLEDAEDSL AIQERWAAEQ ESQYEAQTFE REQQLEVVER LQEVLTQKLS
360
AASEFLQVRE EVF
Length:363
Mass (Da):40,769
Last modified:October 23, 2007 - v2
Checksum:i7CECC42F37A1D705
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 802VH → LD in AAC47029. (PubMed:8626591)Curated
Sequence conflicti343 – 3431E → Q in AAC47029. (PubMed:8626591)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47117 Genomic DNA. Translation: AAC47029.1.
CT867986 Genomic DNA. Translation: CAK56161.1.
RefSeqiXP_001423559.1. XM_001423522.1.
UniGeneiPte.18507.

Genome annotation databases

EnsemblProtistsiCAK56161; CAK56161; GSPATT00004255001.
GeneIDi5009343.
KEGGiptm:GSPATT00004255001.

Cross-referencesi

Web resourcesi

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47117 Genomic DNA. Translation: AAC47029.1 .
CT867986 Genomic DNA. Translation: CAK56161.1 .
RefSeqi XP_001423559.1. XM_001423522.1.
UniGenei Pte.18507.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi CAK56161 ; CAK56161 ; GSPATT00004255001 .
GeneIDi 5009343.
KEGGi ptm:GSPATT00004255001.

Phylogenomic databases

InParanoidi Q27182.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
    Gautier M.-C., Sperling L., Madeddu L.
    J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Stock d4-2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Stock d4-2.
  3. "A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
    Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
    Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-79.
    Strain: Stock d4-2.
  4. "Protein processing and morphogenesis of secretory granules in Paramecium."
    Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
    Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: Stock d4-2.

Entry informationi

Entry nameiT4A_PARTE
AccessioniPrimary (citable) accession number: Q27182
Secondary accession number(s): A0BC94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 23, 2007
Last modified: October 29, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3