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Q27182

- T4A_PARTE

UniProt

Q27182 - T4A_PARTE

Protein

Trichocyst matrix protein T4-A

Gene

T4A

Organism
Paramecium tetraurelia
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 2 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Structural protein that crystallize inside the trichocyst matrix.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trichocyst matrix protein T4-A
    Alternative name(s):
    Secretory granule protein T4-A
    TMP 4-A
    Cleaved into the following 2 chains:
    Gene namesi
    Name:T4A
    ORF Names:GSPATT00004255001
    OrganismiParamecium tetraurelia
    Taxonomic identifieri5888 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
    ProteomesiUP000000600: Partially assembled WGS sequence

    Subcellular locationi

    Trichocyst
    Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

    GO - Cellular componenti

    1. trichocyst Source: UniProtKB-SubCell

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 5235PRO_0000034385Add
    BLAST
    Chaini53 – 189137Trichocyst matrix protein T4-A 1PRO_0000034386Add
    BLAST
    Propeptidei190 – 22132PRO_0000034387Add
    BLAST
    Chaini222 – 363142Trichocyst matrix protein T4-A 2PRO_0000034388Add
    BLAST

    Post-translational modificationi

    Two components are produced by post-translational processing from the precursor peptide.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili85 – 11935Sequence AnalysisAdd
    BLAST
    Coiled coili244 – 352109Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TMP family.Curated

    Keywords - Domaini

    Coiled coil, Signal

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27182-1 [UniParc]FASTAAdd to Basket

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    MARSLTILAI VFAVATARVT KSESPKEILA QVNKDSFGNS ILSVLQLQLA    50
    TGGPVGEIQI LLNNIASQLN GDQKKADKVH ESDTVAFEKI IADLEQEIAY 100
    HQTQIVALSN LRDSTTEALG EAEVEVRVVT SDIANNEKSF ADESATRQSQ 150
    HDTWVRKDAE HVDQMEAIDE ASKIVQHLQA GVAFAQLKSR FEKVQAKLME 200
    SKHALFKPLI NALTQLASKV DNKSIIKILE LLAQIRQQLV ASRASLLATE 250
    ERQAANWEVQ SSHLQEEHKR LVERKAFLEN SIVQFKVTIQ EAVEDLEDQT 300
    LFLEDAEDSL AIQERWAAEQ ESQYEAQTFE REQQLEVVER LQEVLTQKLS 350
    AASEFLQVRE EVF 363
    Length:363
    Mass (Da):40,769
    Last modified:October 23, 2007 - v2
    Checksum:i7CECC42F37A1D705
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti79 – 802VH → LD in AAC47029. (PubMed:8626591)Curated
    Sequence conflicti343 – 3431E → Q in AAC47029. (PubMed:8626591)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47117 Genomic DNA. Translation: AAC47029.1.
    CT867986 Genomic DNA. Translation: CAK56161.1.
    RefSeqiXP_001423559.1. XM_001423522.1.
    UniGeneiPte.18507.

    Genome annotation databases

    EnsemblProtistsiCAK56161; CAK56161; GSPATT00004255001.
    GeneIDi5009343.
    KEGGiptm:GSPATT00004255001.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The arsenal of Paramecium - Issue 3 of October 2000

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47117 Genomic DNA. Translation: AAC47029.1 .
    CT867986 Genomic DNA. Translation: CAK56161.1 .
    RefSeqi XP_001423559.1. XM_001423522.1.
    UniGenei Pte.18507.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi CAK56161 ; CAK56161 ; GSPATT00004255001 .
    GeneIDi 5009343.
    KEGGi ptm:GSPATT00004255001.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
      Gautier M.-C., Sperling L., Madeddu L.
      J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Stock d4-2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Stock d4-2.
    3. "A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
      Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
      Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-79.
      Strain: Stock d4-2.
    4. "Protein processing and morphogenesis of secretory granules in Paramecium."
      Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
      Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Strain: Stock d4-2.

    Entry informationi

    Entry nameiT4A_PARTE
    AccessioniPrimary (citable) accession number: Q27182
    Secondary accession number(s): A0BC94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3