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Q27181 (T2C_PARTE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trichocyst matrix protein T2-C
Alternative name(s):
Secretory granule protein T2-C
TMP 2-C
Gene names
Name:T2C
ORF Names:GSPATT00003811001
OrganismParamecium tetraurelia [Reference proteome]
Taxonomic identifier5888 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Structural protein that crystallize inside the trichocyst matrix.

Subcellular location

Trichocyst. Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

Post-translational modification

Two components are produced by post-translational processing from the precursor peptide.

Sequence similarities

Belongs to the TMP family.

Ontologies

Keywords
   DomainCoiled coil
Signal
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componenttrichocyst

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 4829
PRO_0000034381
Chain49 – 183135Trichocyst matrix protein T2-C 1
PRO_0000034382
Propeptide184 – 23956
PRO_0000034383
Chain240 – 387148Trichocyst matrix protein T2-C 2
PRO_0000034384

Regions

Coiled coil51 – 163113 Potential
Coiled coil294 – 33340 Potential

Sequences

Sequence LengthMass (Da)Tools
Q27181 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D6885D7E22EED1D8

FASTA38743,312
        10         20         30         40         50         60 
MKTIILALAL IVLASSTQAD VIATIKKIDQ SPFGRTLFDT IWLELQTGDP LDRLLQTLTD 

        70         80         90        100        110        120 
LEDRYVAEQK EDDARNHEYQ DACTVDIKAF DKDLAESNRK KIELEARLEG QLYPQRGILQ 

       130        140        150        160        170        180 
GLVAQKQAEV KGYQKDLDEL DAQRAEEKAD FEEKVLEHQE ATAIIAEARR LFADNIEHES 

       190        200        210        220        230        240 
FIQKGKATKQ PAHKFTKEVA SMIQKHFTTS AKKAAKFQHR KGYSKLFKAF ATIASKVEQL 

       250        260        270        280        290        300 
ADAGAVSKII DLADELLAKI ADSLSLLRFA EDKRVEAYKK SRNFIVISLT VAGTALANAQ 

       310        320        330        340        350        360 
SDLAALNDVI AQVEASLDTT NQRIENVSAD RNDRFTQCEE AVQDYQDARS ARSSDRDVVS 

       370        380 
QTIGLVNKEL RTLREQLALR QSAGEEI 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
Gautier M.-C., Sperling L., Madeddu L.
J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Stock d4-2.
[2]"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia."
Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. expand/collapse author list , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P.
Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Stock d4-2.
[3]"A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-72.
Strain: Stock d4-2.
[4]"Protein processing and morphogenesis of secretory granules in Paramecium."
Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Strain: Stock d4-2.

Web resources

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47116 Genomic DNA. Translation: AAC47028.1.
CT868660 Genomic DNA. Translation: CAK90946.1.
U27511 Genomic DNA. Translation: AAA92611.1.
RefSeqXP_001458343.1. XM_001458306.1.
UniGenePte.18177.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsCAK90946; CAK90946; GSPATT00003811001.
GeneID5044128.
KEGGptm:GSPATT00003811001.

Phylogenomic databases

ProtClustDBCLSZ2447196.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2C_PARTE
AccessionPrimary (citable) accession number: Q27181
Secondary accession number(s): A0E6M9, Q27175
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries