Q27181 (T2C_PARTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trichocyst matrix protein T2-C

Alternative name(s):
Secretory granule protein T2-C
TMP 2-C

Cleaved into the following 2 chains:

1. Trichocyst matrix protein T2-C 1
2. Trichocyst matrix protein T2-C 2

Gene names

Name:	T2C
ORF Names:	GSPATT00003811001

Organism

Paramecium tetraurelia [Reference proteome]

Taxonomic identifier

5888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Peniculida › Parameciidae › Paramecium

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural protein that crystallize inside the trichocyst matrix.
Subcellular location	Trichocyst. Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.
Post-translational modification	Two components are produced by post-translational processing from the precursor peptide.
Sequence similarities	Belongs to the TMP family.

Ontologies

Keywords
Domain	Coiled coil Signal
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	trichocyst Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 48	29			PRO_0000034381
	Chain	49 – 183	135	Trichocyst matrix protein T2-C 1		PRO_0000034382
	Propeptide	184 – 239	56			PRO_0000034383
	Chain	240 – 387	148	Trichocyst matrix protein T2-C 2		PRO_0000034384
Regions
	Coiled coil	51 – 163	113	Potential
	Coiled coil	294 – 333	40	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q27181 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D6885D7E22EED1D8	FASTA	387	43,312
	10 20 30 40 50 60 MKTIILALAL IVLASSTQAD VIATIKKIDQ SPFGRTLFDT IWLELQTGDP LDRLLQTLTD 70 80 90 100 110 120 LEDRYVAEQK EDDARNHEYQ DACTVDIKAF DKDLAESNRK KIELEARLEG QLYPQRGILQ 130 140 150 160 170 180 GLVAQKQAEV KGYQKDLDEL DAQRAEEKAD FEEKVLEHQE ATAIIAEARR LFADNIEHES 190 200 210 220 230 240 FIQKGKATKQ PAHKFTKEVA SMIQKHFTTS AKKAAKFQHR KGYSKLFKAF ATIASKVEQL 250 260 270 280 290 300 ADAGAVSKII DLADELLAKI ADSLSLLRFA EDKRVEAYKK SRNFIVISLT VAGTALANAQ 310 320 330 340 350 360 SDLAALNDVI AQVEASLDTT NQRIENVSAD RNDRFTQCEE AVQDYQDARS ARSSDRDVVS 370 380 QTIGLVNKEL RTLREQLALR QSAGEEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures." Gautier M.-C., Sperling L., Madeddu L. J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Stock d4-2.
[2]	"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia." Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P. Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Stock d4-2.
[3]	"A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium." Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L. Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-72. Strain: Stock d4-2.
[4]	"Protein processing and morphogenesis of secretory granules in Paramecium." Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L. Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Stock d4-2.

Web resources

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47116 Genomic DNA. Translation: AAC47028.1. CT868660 Genomic DNA. Translation: CAK90946.1. U27511 Genomic DNA. Translation: AAA92611.1.
RefSeq	XP_001458343.1. XM_001458306.1.
UniGene	Pte.18177.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5044128.
KEGG	ptm:GSPATT00003811001.
Phylogenomic databases
ProtClustDB	CLSZ2447196.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2C_PARTE

Accession

Primary (citable) accession number: Q27181
Secondary accession number(s): A0E6M9, Q27175

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)