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Q27180 (T1B_PARTE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trichocyst matrix protein T1-B
Alternative name(s):
Secretory granule protein T1-B
TMP 1-B
Gene names
Name:T1B
ORF Names:GSPATT00027326001
OrganismParamecium tetraurelia [Reference proteome]
Taxonomic identifier5888 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Structural protein that crystallize inside the trichocyst matrix.

Subcellular location

Trichocyst. Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

Post-translational modification

Two components are produced by post-translational processing from the precursor peptide.

Sequence similarities

Belongs to the TMP family.

Ontologies

Keywords
   DomainCoiled coil
Signal
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componenttrichocyst

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Propeptide17 – 5539
PRO_0000034377
Chain56 – 189134Trichocyst matrix protein T1-B 1
PRO_0000034378
Propeptide190 – 22536
PRO_0000034379
Chain226 – 369144Trichocyst matrix protein T1-B 2
PRO_0000034380

Regions

Coiled coil56 – 180125 Potential
Coiled coil262 – 35493 Potential

Sequences

Sequence LengthMass (Da)Tools
Q27180 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 82CFD93E424BA3AD

FASTA36941,963
        10         20         30         40         50         60 
MYKLAVCTLL ILSVTAIDVT NSVWTSHDQK AFAQIKQSGW GNFILNFGEL HLQTGGILAE 

        70         80         90        100        110        120 
LNTEIAKLID ELDEELAEVH HQYARRTDVH NREVSRLEQE IQDKEREVFN AHDFYDNVLI 

       130        140        150        160        170        180 
PQRDRFAAQL EQLQENIAQN RRTLNEATVQ RANDHAEFES QVAEHNEAIS AIDESLQLLS 

       190        200        210        220        230        240 
QLESPSLVQI QKVQKNLTKI QQSLKRHSTF QTFIKTLLEI AVEANFADQG ALREILTAFN 

       250        260        270        280        290        300 
NLRVQLVDSL NQLTADEAEA QKDFEARVIQ LNQEHAEFQR AVVVKTAEIE ANANKIEQTL 

       310        320        330        340        350        360 
DLIDVLHADL DTLNGQLQAE NDDYAFATDV YNATVSEYNK ELNAAHQALD LLNQPRFTDY 


VKSQLKGAF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
Gautier M.-C., Sperling L., Madeddu L.
J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Stock d4-2.
[2]"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia."
Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. expand/collapse author list , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P.
Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Stock d4-2.
[3]"A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-259.
Strain: Stock d4-2.
[4]"Protein processing and morphogenesis of secretory granules in Paramecium."
Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Strain: Stock d4-2.

Web resources

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47115 Genomic DNA. Translation: AAC47027.1.
CT868680 Genomic DNA. Translation: CAK94998.1.
U27504 Genomic DNA. Translation: AAA92604.1.
RefSeqXP_001462371.1. XM_001462334.1.
UniGenePte.28170.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsCAK94998; CAK94998; GSPATT00027326001.
GeneID5048156.
KEGGptm:GSPATT00027326001.

Phylogenomic databases

ProtClustDBCLSZ2447676.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT1B_PARTE
AccessionPrimary (citable) accession number: Q27180
Secondary accession number(s): A0EI57, Q27247
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries