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Q27180

- T1B_PARTE

UniProt

Q27180 - T1B_PARTE

Protein

Trichocyst matrix protein T1-B

Gene

T1B

Organism
Paramecium tetraurelia
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Structural protein that crystallize inside the trichocyst matrix.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trichocyst matrix protein T1-B
    Alternative name(s):
    Secretory granule protein T1-B
    TMP 1-B
    Cleaved into the following 2 chains:
    Gene namesi
    Name:T1B
    ORF Names:GSPATT00027326001
    OrganismiParamecium tetraurelia
    Taxonomic identifieri5888 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
    ProteomesiUP000000600: Partially assembled WGS sequence

    Subcellular locationi

    Trichocyst
    Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.

    GO - Cellular componenti

    1. trichocyst Source: UniProtKB-SubCell

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Propeptidei17 – 5539PRO_0000034377Add
    BLAST
    Chaini56 – 189134Trichocyst matrix protein T1-B 1PRO_0000034378Add
    BLAST
    Propeptidei190 – 22536PRO_0000034379Add
    BLAST
    Chaini226 – 369144Trichocyst matrix protein T1-B 2PRO_0000034380Add
    BLAST

    Post-translational modificationi

    Two components are produced by post-translational processing from the precursor peptide.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili56 – 180125Sequence AnalysisAdd
    BLAST
    Coiled coili262 – 35493Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TMP family.Curated

    Keywords - Domaini

    Coiled coil, Signal

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27180-1 [UniParc]FASTAAdd to Basket

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    MYKLAVCTLL ILSVTAIDVT NSVWTSHDQK AFAQIKQSGW GNFILNFGEL    50
    HLQTGGILAE LNTEIAKLID ELDEELAEVH HQYARRTDVH NREVSRLEQE 100
    IQDKEREVFN AHDFYDNVLI PQRDRFAAQL EQLQENIAQN RRTLNEATVQ 150
    RANDHAEFES QVAEHNEAIS AIDESLQLLS QLESPSLVQI QKVQKNLTKI 200
    QQSLKRHSTF QTFIKTLLEI AVEANFADQG ALREILTAFN NLRVQLVDSL 250
    NQLTADEAEA QKDFEARVIQ LNQEHAEFQR AVVVKTAEIE ANANKIEQTL 300
    DLIDVLHADL DTLNGQLQAE NDDYAFATDV YNATVSEYNK ELNAAHQALD 350
    LLNQPRFTDY VKSQLKGAF 369
    Length:369
    Mass (Da):41,963
    Last modified:November 1, 1996 - v1
    Checksum:i82CFD93E424BA3AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47115 Genomic DNA. Translation: AAC47027.1.
    CT868680 Genomic DNA. Translation: CAK94998.1.
    U27504 Genomic DNA. Translation: AAA92604.1.
    RefSeqiXP_001462371.1. XM_001462334.1.
    UniGeneiPte.28170.

    Genome annotation databases

    EnsemblProtistsiCAK94998; CAK94998; GSPATT00027326001.
    GeneIDi5048156.
    KEGGiptm:GSPATT00027326001.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The arsenal of Paramecium - Issue 3 of October 2000

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47115 Genomic DNA. Translation: AAC47027.1 .
    CT868680 Genomic DNA. Translation: CAK94998.1 .
    U27504 Genomic DNA. Translation: AAA92604.1 .
    RefSeqi XP_001462371.1. XM_001462334.1.
    UniGenei Pte.28170.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi CAK94998 ; CAK94998 ; GSPATT00027326001 .
    GeneIDi 5048156.
    KEGGi ptm:GSPATT00027326001.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of genes coding for paramecium secretory granule (trichocyst) proteins. A unique protein fold for a family of polypeptides with different primary structures."
      Gautier M.-C., Sperling L., Madeddu L.
      J. Biol. Chem. 271:10247-10255(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Stock d4-2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Stock d4-2.
    3. "A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium."
      Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.
      Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-259.
      Strain: Stock d4-2.
    4. "Protein processing and morphogenesis of secretory granules in Paramecium."
      Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.
      Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Strain: Stock d4-2.

    Entry informationi

    Entry nameiT1B_PARTE
    AccessioniPrimary (citable) accession number: Q27180
    Secondary accession number(s): A0EI57, Q27247
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3