Q27174 (T2B_PARTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trichocyst matrix protein T2-B

Alternative name(s):
Secretory granule protein T2-B
TMP 2-B

Cleaved into the following 2 chains:

1. Trichocyst matrix protein T2-B 1
2. Trichocyst matrix protein T2-B 2

Gene names

Name:	T2B
ORF Names:	GSPATT00011919001

Organism

Paramecium tetraurelia [Reference proteome]

Taxonomic identifier

5888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Peniculida › Parameciidae › Paramecium

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural protein that crystallize inside the trichocyst matrix.
Subcellular location	Trichocyst. Note: These are architecturally complex secretory storage granules-docked at the plasma membrane, ready to rapidly respond to an exocytotic stimulus.
Sequence similarities	Belongs to the TMP family.

Ontologies

Keywords
Domain	Coiled coil Signal
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	trichocyst Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 48	29	By similarity		PRO_0000307849
	Chain	49 – 183	135	Trichocyst matrix protein T2-B 1		PRO_0000221509
	Propeptide	184 – 238	55	By similarity		PRO_0000307850
	Chain	239 – 386	148	Trichocyst matrix protein T2-B 2		PRO_0000307851
Regions
	Coiled coil	51 – 154	104	Potential
	Coiled coil	294 – 325	32	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q27174 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: C62BF2AEE2A36A40	FASTA	386	43,292
	10 20 30 40 50 60 MKTIILALAL IALVSSTQSD VIDTIKKIDQ SPFGRTLFDT IWLELQTGDP LDRLVSTLTD 70 80 90 100 110 120 LEDRYVAEQK EDDAKNQEYQ GACTVDITAF DKDLAESNRK KIELEARLEG QLYPQRSILE 130 140 150 160 170 180 GLVAQKKAEV KGYQKDLDEL DAQRAEEHED FEEKVLEHQE ATAIIAEARR LFADNIEHES 190 200 210 220 230 240 FVQKGKAAKQ PHKFTKDVAN LIQKHFTTSA KKTAKFQHRK GYSKLFKAFA TIASKVEQLA 250 260 270 280 290 300 DAGAVQKIID LADELLAKIS DSLSLLRFAE DKRVESYKKS RNFVVIALNV AGSALANAIS 310 320 330 340 350 360 DLAALNDIIA QVEASLDTTV QRIENVSADR HDRFTQCEEA VQDYSDARSA RQSDRDVVSQ 370 380 TIGLVNKELR TLREQLALRQ QAGEQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia." Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P. Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Stock d4-2.
[2]	"A large multigene family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium." Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L. Mol. Biol. Cell 6:649-659(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-71. Strain: Stock d4-2.
[3]	"Protein processing and morphogenesis of secretory granules in Paramecium." Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L. Biochimie 76:329-335(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Stock d4-2.

Web resources

Protein Spotlight

The arsenal of Paramecium - Issue 3 of October 2000

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT868230 Genomic DNA. Translation: CAK76346.1. U27510 Genomic DNA. Translation: AAA92610.1.
RefSeq	XP_001443743.1. XM_001443706.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5029528.
KEGG	ptm:GSPATT00011919001.
Phylogenomic databases
ProtClustDB	CLSZ2447196.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2B_PARTE

Accession

Primary (citable) accession number: Q27174
Secondary accession number(s): A0CZX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	October 23, 2007
Last modified:	April 3, 2013
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)