ID   T1F_PARTE               Reviewed;         368 AA.
AC   Q27172; A0BE79;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 2.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Trichocyst matrix protein T1-F;
DE   AltName: Full=Secretory granule protein T1-F;
DE   AltName: Full=TMP 1-F;
DE   Contains:
DE     RecName: Full=Trichocyst matrix protein T1-F 1;
DE   Contains:
DE     RecName: Full=Trichocyst matrix protein T1-F 2;
DE   Flags: Precursor;
GN   Name=T1F; ORFNames=GSPATT00027878001;
OS   Paramecium tetraurelia.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX   NCBI_TaxID=5888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Stock d4-2;
RX   PubMed=17086204; DOI=10.1038/nature05230;
RA   Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M.,
RA   Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A.,
RA   Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R.,
RA   Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F.,
RA   Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H.,
RA   Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M.,
RA   Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E.,
RA   Cohen J., Wincker P.;
RT   "Global trends of whole-genome duplications revealed by the ciliate
RT   Paramecium tetraurelia.";
RL   Nature 444:171-178(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-258.
RC   STRAIN=Stock d4-2;
RX   PubMed=7579685; DOI=10.1091/mbc.6.6.649;
RA   Madeddu L., Gautier M.-C., Vayssie L., Houari A., Sperling L.;
RT   "A large multigene family codes for the polypeptides of the crystalline
RT   trichocyst matrix in Paramecium.";
RL   Mol. Biol. Cell 6:649-659(1995).
RN   [3]
RP   PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=Stock d4-2;
RX   PubMed=7819344; DOI=10.1016/0300-9084(94)90167-8;
RA   Madeddu L., Gautier M.-C., le Caer J.-P., de Loubresse N., Sperling L.;
RT   "Protein processing and morphogenesis of secretory granules in
RT   Paramecium.";
RL   Biochimie 76:329-335(1994).
CC   -!- FUNCTION: Structural protein that crystallize inside the trichocyst
CC       matrix.
CC   -!- SUBCELLULAR LOCATION: Trichocyst. Note=These are architecturally
CC       complex secretory storage granules-docked at the plasma membrane, ready
CC       to rapidly respond to an exocytotic stimulus.
CC   -!- SIMILARITY: Belongs to the TMP family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=The arsenal of Paramecium
CC       - Issue 3 of October 2000;
CC       URL="https://web.expasy.org/spotlight/back_issues/003";
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DR   EMBL; CT867988; CAK56846.1; -; Genomic_DNA.
DR   EMBL; U27508; AAA92608.1; -; Genomic_DNA.
DR   RefSeq; XP_001424244.1; XM_001424207.1.
DR   AlphaFoldDB; Q27172; -.
DR   SMR; Q27172; -.
DR   EnsemblProtists; CAK56846; CAK56846; GSPATT00027878001.
DR   GeneID; 5010028; -.
DR   KEGG; ptm:GSPATT00027878001; -.
DR   HOGENOM; CLU_065271_0_0_1; -.
DR   InParanoid; Q27172; -.
DR   Proteomes; UP000000600; Partially assembled WGS sequence.
DR   GO; GO:0055039; C:trichocyst; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Coiled coil; Direct protein sequencing; Reference proteome; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   PROPEP          17..55
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000307839"
FT   CHAIN           56..189
FT                   /note="Trichocyst matrix protein T1-F 1"
FT                   /id="PRO_0000307840"
FT   PROPEP          190..225
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000307841"
FT   CHAIN           226..368
FT                   /note="Trichocyst matrix protein T1-F 2"
FT                   /id="PRO_0000221507"
FT   COILED          56..153
FT                   /evidence="ECO:0000255"
FT   COILED          257..354
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   368 AA;  41481 MW;  90DCDD3240141F06 CRC64;
     MFKVAVCALL VLASTAINVQ SSIWTSKDQK AFAQIHQSGW GKFILNFAEL HLTTGGILSE
     LNSEIEKLIG EMEEELAGVH HEFNRRTDVH NREVARLEQE IQDKERELFN AHDFYDNVLI
     PQGERFAAQL EQLQENIAHN RQTLEQATVQ RANDHETFES EVVEHNDAIS AIDECLQLLS
     TIAAPSLAEV KKVQKNLAKI QNSLKKHNQF QIFVKVLLEI TVDSNFADQG ALRDIIVAFN
     NLRVELVDSL NQITADEAEQ VADYNAQVIA LNQEHAEFQR AVVVKNAEIE ANTTKQEQTL
     DLIDELDEDL ATLNGQLQAE NDDYAFATDV YNATVAEYNK EINAANQALD LLNQPRFQDY
     VKSQLKGA
//