Reviewed, UniProtKB/Swiss-Prot Q27032 (CDC2H_THEPA)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	CRK2
Ordered Locus Names:	TP01_0728

Organism

Theileria parva [Complete proteome]

Taxonomic identifier

5875 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Piroplasmida › Theileriidae › Theileria

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-158 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Cell division control protein 2 homolog		PRO_0000232674
Regions
	Domain	4 – 284	281	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	125	1	Proton acceptor By similarity
	Binding site	32	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	158	1	Phosphothreonine; by CAK By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27032-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0C25B84458B1341C	FASTA	298	34,213
	10 20 30 40 50 60 MRRYHKMEKI GEGTYGVVYK AQNNHGEICA LKKIRVEEED EGIPSTAIRE ISLLKELHHP 70 80 90 100 110 120 NIVWLRDVIH SEKCLTLVFE YLDQDLKKLL DACDGGLEPT TAKSFLYQIL RGISYCHDHR 130 140 150 160 170 180 ILHRDLKPQN LLINREGVLK LADFGLARAF AIPVRSYTHE VVTLWYRAPD VLMGSKKYST 190 200 210 220 230 240 AVDIWSVGCI FAEMINGVPL FPGISEQDQL KRIFKILGTP SVDSWPQVVN LPAYNPDFSY 250 260 270 280 290 YEKQSWSSIV PKLNESGIDL ISRMLQLDPV QRISAKEALK HDYFKDLHRP PEFLNGVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation and characterisation of genomic and cDNA clones coding for a cdc2-related kinase (ThCRK2) from the bovine protozoan parasite Theileria." Kinnaird J.H., Logan M., Kirvar E., Tait A., Carrington M. Mol. Microbiol. 22:293-302(1996) [PubMed: 8930914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Muguga.
[2]	"Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes." Gardner M.J., Bishop R., Shah T., de Villiers E.P., Carlton J.M., Hall N., Ren Q., Paulsen I.T., Pain A., Berriman M., Wilson R.J.M., Sato S., Ralph S.A., Mann D.J., Xiong Z., Shallom S.J., Weidman J., Jiang L. , Lynn J., Weaver B., Shoaibi A., Domingo A.R., Wasawo D., Crabtree J., Wortman J.R., Haas B., Angiuoli S.V., Creasy T.H., Lu C., Suh B., Silva J.C., Utterback T.R., Feldblyum T.V., Pertea M., Allen J., Nierman W.C., Taracha E.L.N., Salzberg S.L., White O.R., Fitzhugh H.A., Morzaria S., Venter J.C., Fraser C.M., Nene V. Science 309:134-137(2005) [PubMed: 15994558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Muguga.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98824 Genomic DNA. Translation: CAA67342.1. AAGK01000001 Genomic DNA. Translation: EAN33966.1.
RefSeq	XP_766249.1.
3D structure databases
SMR	Q27032. Positions 1-286.
ModBase	Search...
Genome annotation databases
GeneID	3503486.
KEGG	tpv:TP01_0728.
Phylogenomic databases
PhylomeDB	Q27032.
Enzyme and pathway databases
BRENDA	2.7.11.22. 288010. 2.7.11.23. 288010.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2H_THEPA

Accession

Primary (citable) accession number: Q27032

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)