Reviewed, UniProtKB/Swiss-Prot Q26783 (NDUS7_TRYBB)

Names and origin

Protein names

Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 20 kDa subunit
    Complex I-20kD
      Short name=CI-20kD

Gene names

Name:

NDHK

Organism

Trypanosoma brucei brucei

Taxonomic identifier

5702 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 56	56	Mitochondrion Potential
	Chain	57 – 202	146	NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial		PRO_0000020031
Sites
	Metal binding	77	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	78	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	142	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	172	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q26783-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 58CC3911E359E50D	FASTA	202	23,077
	10 20 30 40 50 60 MLRRTSFNFT GRAMISRGSP EWSHRLDLKK GKKTTMMHKL GTSKPNNALQ YAQMTLHDLT 70 80 90 100 110 120 EWCLAYSPWP LTFGLACCAV EMMHAYASRY DLDRFGIVPR PTPRQAEIII VSGTVTNKMA 130 140 150 160 170 180 PILRNIYVQM VNPKWVISMG SCANGGGYYH FSYAVLRGCE RAIPVDFWIP GCPPSAESLV 190 200 FCLHTLQKKI RWHEIQKYSV RD

References

[1]

"Characterization of a Trypanosoma brucei nuclear gene encoding a protein homologous to a subunit of bovine NADH:ubiquinone oxidoreductase (complex I)." Peterson G.C., Souza A.E., Parsons M. Mol. Biochem. Parasitol. 58:63-70(1993) [PubMed: 8459836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EATRO 164.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L00585 Genomic DNA. Translation: AAA30217.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS7_TRYBB

Accession

Primary (citable) accession number: Q26783

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)