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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Gene

NDHK

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi78 – 781Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi142 – 1421Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi172 – 1721Iron-sulfur (4Fe-4S)Sequence Analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-20kD
Short name:
CI-20kD
NADH-ubiquinone oxidoreductase 20 kDa subunit
Gene namesi
Name:NDHK
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5656MitochondrionSequence AnalysisAdd
BLAST
Chaini57 – 202146NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrialPRO_0000020031Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

STRINGi5691.EAN79111.

Structurei

3D structure databases

ProteinModelPortaliQ26783.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q26783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRRTSFNFT GRAMISRGSP EWSHRLDLKK GKKTTMMHKL GTSKPNNALQ
60 70 80 90 100
YAQMTLHDLT EWCLAYSPWP LTFGLACCAV EMMHAYASRY DLDRFGIVPR
110 120 130 140 150
PTPRQAEIII VSGTVTNKMA PILRNIYVQM VNPKWVISMG SCANGGGYYH
160 170 180 190 200
FSYAVLRGCE RAIPVDFWIP GCPPSAESLV FCLHTLQKKI RWHEIQKYSV

RD
Length:202
Mass (Da):23,077
Last modified:November 1, 1996 - v1
Checksum:i58CC3911E359E50D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00585 Genomic DNA. Translation: AAA30217.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00585 Genomic DNA. Translation: AAA30217.1.

3D structure databases

ProteinModelPortaliQ26783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5691.EAN79111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a Trypanosoma brucei nuclear gene encoding a protein homologous to a subunit of bovine NADH:ubiquinone oxidoreductase (complex I)."
    Peterson G.C., Souza A.E., Parsons M.
    Mol. Biochem. Parasitol. 58:63-70(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EATRO 164.

Entry informationi

Entry nameiNDUS7_TRYBB
AccessioniPrimary (citable) accession number: Q26783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.