Reviewed, UniProtKB/Swiss-Prot Q26721 (CYA4_TRYBB)

Names and origin

Protein names

Recommended name:
    Receptor-type adenylate cyclase ESAG 4
    EC=4.6.1.1
Alternative name(s):
    ATP pyrophosphate-lyase
    Adenylyl cyclase
    Expression site-associated protein 4
    Antigen 4

Gene names

Name:

ESAG4

Organism

Trypanosoma brucei brucei

Taxonomic identifier

5702 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	1235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could act as a receptor for a unknown ligand.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase Receptor
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1235	1235	Receptor-type adenylate cyclase ESAG 4		PRO_0000195736
Regions
	Topological domain	1 – 32	32	Cytoplasmic Potential
	Transmembrane	33 – 53	21	Potential
	Topological domain	54 – 858	805	Extracellular Potential
	Transmembrane	859 – 879	21	Potential
	Topological domain	880 – 1235	356	Cytoplasmic Potential
	Domain	900 – 1054	155	Guanylate cyclase
	Compositional bias	46 – 50	5	Poly-Leu
Sites
	Metal binding	905	1	Magnesium By similarity
	Metal binding	948	1	Magnesium By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential
	Glycosylation	531	1	N-linked (GlcNAc...) Potential
	Glycosylation	566	1	N-linked (GlcNAc...) Potential
	Glycosylation	705	1	N-linked (GlcNAc...) Potential
	Glycosylation	830	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q26721-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CCAC3E2D78D3DC7A	FASTA	1,235	136,458
	10 20 30 40 50 60 MNMLHLSDRN ASLAPSGGEH SLPTGGAVCR DAMDILPVIL RAPVALLLLL VVLPQLSVGA 70 80 90 100 110 120 EANVTVKVLS ATWNWYMPRK YVTAINAGFN ASLKSRNWTV AGSVNVQVVY PSNLDLMPED 130 140 150 160 170 180 FIKKQLELET DQNKIVIVYG PLGDKSVMHS IPHLMNHRVV AFGLITGSTF IRQWNPYLYF 190 200 210 220 230 240 LRADPAAETL VLIRYSLCQL RVLRLGFMYL QGVHYGDEEY ALTVNVMSRM GYELHGVFTV 250 260 270 280 290 300 MSPDGKPAPD AEFKEVFERF ATALPQAIIV FGAPVDDTAK FLMMMAVDER IARSYILSPS 310 320 330 340 350 360 SVQLSLIEMW QLALEAAGAS FAPGQLLFTG TNPLAKDSQY TAIKRFQEVM SEYLKAHVGE 370 380 390 400 410 420 TNITEADYFL THDLEGELMV YGWISGEVLS QALSNLEWLK DRATFVRSLY DQRRYVINDI 430 440 450 460 470 480 VIGDYGGTCE GDAAKHGATC ECNQGSKAVY VKEMLENGQK TSVRSGFTVL KASLCYTDSS 490 500 510 520 530 540 ELHGPLDGLV VFMKDDDIAS KAAALWQKGT SHLVGKGDLG YSDRFFLHAF NTTIAEAAND 550 560 570 580 590 600 LRRDQGERIV TAVFGPVTEA MLDTPNITFI DPLELKPRLN KFRRNVIHLS PTLEQQLYVL 610 620 630 640 650 660 SSYLAGAGVG NVDAVICSNE ADGIADFLRS SLTEFAVSLR SAVIREDGED VGKYLPMSGT 670 680 690 700 710 720 VFVIGLSVPD VKEIARKLEE RNDLRVIVLF GEFSFLYDLF ATALNNTAGA ARLVFATSLP 730 740 750 760 770 780 HWGDTETSSK TAQLFHDVEK DSRLWTPLSV LAFATGRLMR VILLHVEEMS PETLVNFFYT 790 800 810 820 830 840 DSSIVSDDMR YGVFDDTKCV DTANKLSKNG CASNYGATQI SVWSMARALN ASIPPLTNPM 850 860 870 880 890 900 TPSMTFRNSN AGRISGASLV GIIIGGALAL FLVVALGVVP YFFLRNTVIT ICTKDDRPVT 910 920 930 940 950 960 LIFTDIESST ALWAAHPEVM PDAVATHHRL IRTLISKYEC YEVKTVGDSF MIASKSPFAA 970 980 990 1000 1010 1020 VQLAQELQLC FLHHDWGTNA IDESYQQFEQ QRAEDDSDYT PPTARLDPKV YSRLWNGLRV 1030 1040 1050 1060 1070 1080 RVGIHTGLCD IRRDEVTKGY DYYGRTSNMA ARTESVANGG QVLMTHAAYM SLSAEERQQI 1090 1100 1110 1120 1130 1140 DVTALGDVPL RGVPKPVEMY RLNAVPGRTF SVLRLELELL NDDEDQTTTS CSDHSSSRTD 1150 1160 1170 1180 1190 1200 LSVAAQTIAA SLQSLLGTFT PAQRQKALIP FCERWRVPLP QKVGNVWDDD GCQEVVRRVA 1210 1220 1230 AKVGRVMDFG TRKPSSSVTS WKGVEVSSQV EERLL

References

[1]	"The genes and transcripts of an antigen gene expression site from T. brucei." Pays E., Tebabi P., Pays A., Coquelet H., Revelard P., Salmon D., Steinert M. Cell 57:835-845(1989) [PubMed: 2720787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EATRO 1125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33720 Genomic DNA. Translation: AAA30158.1.
PIR	D32433.
3D structure databases
SMR	Q26721. Positions 816-1103, 898-1121.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.6.1.1. 74165.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit.
Pfam	PF00211. Guanylate_cyc. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYA4_TRYBB

Accession

Primary (citable) accession number: Q26721

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)