Q26680 (FCA2_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Flagellar calcium-binding protein TB-24 Alternative name(s): 24 kDa calcimedin 24 kDa calflagin
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Trypanozoon › Trypanosoma brucei

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May contribute to the rapid motility of the trypanosomes, playing a role either in flagellar structure or in calcium metabolism. Could alternate between a GDP-bound inactive form to a calcium/GTP-bound active form.
Subcellular location	Cell projection › cilium › flagellum.
Domain	This protein has four EF-hand domains, three of which may be functional calcium-binding sites.
Sequence similarities	Belongs to the calflagin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell projection Cilium Flagellum
Domain	Repeat
Ligand	Calcium Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Cellular_component	cilium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 218

218

Flagellar calcium-binding protein TB-24

PRO_0000073737

Regions

Domain

48 – 83

36

EF-hand 1

Domain

84 – 119

36

EF-hand 2

Domain

130 – 165

36

EF-hand 3

Domain

167 – 202

36

EF-hand 4

Calcium binding

61 – 72

12

1 Potential

Calcium binding

143 – 154

12

2 Potential

Calcium binding

180 – 191

12

3 Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

218

Helix Strand Turn

Details...

Beta strand

20 – 22

3

Helix

27 – 40

14

Helix

47 – 60

14

Helix

61 – 63

3

Helix

71 – 79

9

Turn

84 – 86

3

Beta strand

87 – 89

3

Helix

91 – 108

18

Helix

119 – 142

24

Helix

152 – 165

14

Helix

173 – 179

7

Helix

189 – 204

16

Beta strand

209 – 211

3

Sequences

	Sequence		Length	Mass (Da)
	Q26680 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 041152AC2BDD6DDB	FASTA	218	24,252
	10 20 30 40 50 60 MGCSASKDTT NSKDGAASKG GKDGKTTADR KVAWERIRCA IPRDKDAESK SRRIELFKQF 70 80 90 100 110 120 DTNGTGKLGF REVLDGCYGI LKLDEFTTHL PDIVQRAFDK AKDLGNKVKG VGEEDLVEFL 130 140 150 160 170 180 EFRLMLCYIY DIFELTVMFD TMDKDGSLLL ELQEFKEALP KLKEWGVDIT DATTVFNEID 190 200 210 TNGSGVVTFD EFSCWAVTKK LQVCGDPDGE ENGANEGN

References

[1]	"The gene family of EF-hand calcium-binding proteins from the flagellum of Trypanosoma brucei." Wu Y., Deford J., Benjamin R., Lee M.G.-S., Ruben L. Biochem. J. 304:833-841(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 427.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U06644 Genomic DNA. Translation: AAB40004.1.

PIR

S53354.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LVV	NMR	-	A	1-218	[»]

ProteinModelPortal

Q26680.

SMR

Q26680. Positions 22-208.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

eggNOG

NOG43316.

Family and domain databases

Gene3D

1.10.238.10. 2 hits.

InterPro

IPR003299. Calflagin-bd.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF13405. EF_hand_4. 1 hit.
PF13499. EF_hand_5. 1 hit.
[Graphical view]

PRINTS

PR01362. CALFLAGIN.

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FCA2_TRYBB

Accession

Primary (citable) accession number: Q26680

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)