Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cathepsin L

Gene
N/A
Organism
Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important for the overall degradation of proteins in lysosomes. Required for differentiation of imaginal disks.1 Publication

Catalytic activityi

Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461By similarity
Active sitei285 – 2851By similarity
Active sitei306 – 3061By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Differentiation

Protein family/group databases

MEROPSiC01.092.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin L (EC:3.4.22.15)
Cleaved into the following 2 chains:
OrganismiSarcophaga peregrina (Flesh fly) (Boettcherisca peregrina)
Taxonomic identifieri7386 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaOestroideaSarcophagidaeSarcophagaBoettcherisca

Subcellular locationi

  • Lysosome 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Propeptidei18 – 121104Activation peptide1 PublicationPRO_0000026269Add
BLAST
Chaini122 – 294173Cathepsin L heavy chainPRO_0000026270Add
BLAST
Propeptidei295 – 2984By similarityPRO_0000026271
Chaini299 – 33941Cathepsin L light chainPRO_0000026272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi143 ↔ 186By similarity
Disulfide bondi177 ↔ 219By similarity
Disulfide bondi278 ↔ 328Interchain (between heavy and light chains)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

Highly expressed during embryonic development with higher levels in first instar than in third instar.1 Publication

Interactioni

Subunit structurei

Dimer of a heavy and a light chain linked by disulfide bonds.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ26636.
SMRiQ26636. Positions 24-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q26636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTVLVALLA LVALTQAISP LDLIKEEWHT YKLQHRKNYA NEVEERFRMK
60 70 80 90 100
IFNENRHKIA KHNQLFAQGK VSYKLGLNKY ADMLHHEFKE TMNGYNHTLR
110 120 130 140 150
QLMRERTGLV GATYIPPAHV TVPKSVDWRE HGAVTGVKDQ GHCGSCWAFS
160 170 180 190 200
STGALEGQHF RKAGVLVSLS EQNLVDCSTK YGNNGCNGGL MDNAFRYIKD
210 220 230 240 250
NGGIDTEKSY PYEGIDDSCH FNKATIGATD TGFVDIPEGD EEKMKKAVAT
260 270 280 290 300
MGPVSVAIDA SHESFQLYSE GVYNEPECDE QNLDHGVLVV GYGTDESGMD
310 320 330
YWLVKNSWGT TWGEQGYIKM ARNQNNQCGI ATASSYPTV
Length:339
Mass (Da):37,847
Last modified:November 1, 1996 - v1
Checksum:i7401F3281A2FDA33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16533 mRNA. Translation: BAA03970.1.
PIRiA53810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16533 mRNA. Translation: BAA03970.1.
PIRiA53810.

3D structure databases

ProteinModelPortaliQ26636.
SMRiQ26636. Positions 24-339.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC01.092.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATL_SARPE
AccessioniPrimary (citable) accession number: Q26636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.