Reviewed, UniProtKB/Swiss-Prot Q26636 (CATL_SARPE)

Names and origin

Protein names	Recommended name:     Cathepsin L     EC=3.4.22.15 Cleaved into the following 2 chains:     1- Recommended name:             Cathepsin L heavy chain     2- Recommended name:             Cathepsin L light chain
Organism	Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina)
Taxonomic identifier	7386 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Oestroidea › Sarcophagidae › Sarcophaga › Boettcherisca

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes. Required for differentiation of imaginal disks. Ref.1
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds By similarity.
Subcellular location	Lysosome. Ref.1
Developmental stage	Highly expressed during embryonic development with higher levels in first instar than in third instar. Ref.1
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Lysosome
Domain	Signal
Molecular function	Developmental protein Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.1
	Propeptide	18 – 121	104	Activation peptide		PRO_0000026269
	Chain	122 – 294	173	Cathepsin L heavy chain		PRO_0000026270
	Propeptide	295 – 298	4	By similarity		PRO_0000026271
	Chain	299 – 339	41	Cathepsin L light chain		PRO_0000026272
Sites
	Active site	146	1	By similarity
	Active site	285	1	By similarity
	Active site	306	1	By similarity
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Disulfide bond	143 ↔ 186		By similarity
	Disulfide bond	177 ↔ 219		By similarity
	Disulfide bond	278 ↔ 328		Interchain (between heavy and light chains) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q26636-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7401F3281A2FDA33	FASTA	339	37,847
	10 20 30 40 50 60 MRTVLVALLA LVALTQAISP LDLIKEEWHT YKLQHRKNYA NEVEERFRMK IFNENRHKIA 70 80 90 100 110 120 KHNQLFAQGK VSYKLGLNKY ADMLHHEFKE TMNGYNHTLR QLMRERTGLV GATYIPPAHV 130 140 150 160 170 180 TVPKSVDWRE HGAVTGVKDQ GHCGSCWAFS STGALEGQHF RKAGVLVSLS EQNLVDCSTK 190 200 210 220 230 240 YGNNGCNGGL MDNAFRYIKD NGGIDTEKSY PYEGIDDSCH FNKATIGATD TGFVDIPEGD 250 260 270 280 290 300 EEKMKKAVAT MGPVSVAIDA SHESFQLYSE GVYNEPECDE QNLDHGVLVV GYGTDESGMD 310 320 330 YWLVKNSWGT TWGEQGYIKM ARNQNNQCGI ATASSYPTV

References

[1]

"Purification, characterization, and cDNA cloning of procathepsin L from the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina (flesh fly), and its involvement in the differentiation of imaginal discs." Homma K., Kurata S., Natori S. J. Biol. Chem. 269:15258-15264(1994) [PubMed: 8195162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-27; 122-135; 139-154; 200-208; 224-239 AND 306-319, FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D16533 mRNA. Translation: BAA03970.1.
PIR	A53810.
3D structure databases
HSSP	HSSP built from PDB template 1CJL based on UniProtKB P07711.
ModBase	Search...
Protein family/group databases
MEROPS	C01.092.
Enzyme and pathway databases
BRENDA	3.4.22.15. 67068.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL_SARPE

Accession

Primary (citable) accession number: Q26636

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)