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Protein

Sperm receptor for egg jelly

Gene

REJ

Organism
Strongylocentrotus purpuratus (Purple sea urchin)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the egg jelly ligands inducing the acrosome reaction. Could be a regulator of sperm ion channels.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm receptor for egg jelly
Short name:
suREJ
Gene namesi
Name:REJ
OrganismiStrongylocentrotus purpuratus (Purple sea urchin)
Taxonomic identifieri7668 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEchinodermataEleutherozoaEchinozoaEchinoideaEuechinoideaEchinaceaEchinoidaStrongylocentrotidaeStrongylocentrotus
ProteomesiUP000007110 Componenti: Unplaced

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Present on the surface of the sperm flagellum and also on a thin belt of membrane directly over the acrosomal granule at the anterior apex of the sperm head.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 14141388ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1415 – 143521HelicalSequence AnalysisAdd
BLAST
Topological domaini1436 – 145015CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 14501424Sperm receptor for egg jellyPRO_0000024301Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 42By similarity
Disulfide bondi36 ↔ 51By similarity
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi53 ↔ 62By similarity
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi98 ↔ 189By similarity
Disulfide bondi165 ↔ 181By similarity
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi201 ↔ 214By similarity
Disulfide bondi231 ↔ 323By similarity
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi297 ↔ 315By similarity
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi661 – 6611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi677 – 6771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi685 – 6851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi753 – 7531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi842 – 8421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1311 – 13111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1344 – 13441N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed at the earliest stage of spermiogenesis.

Interactioni

Protein-protein interaction databases

STRINGi7668.Q26627.

Structurei

3D structure databases

ProteinModelPortaliQ26627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6337EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini77 – 190114C-type lectin 1PROSITE-ProRule annotationAdd
BLAST
Domaini210 – 324115C-type lectin 2PROSITE-ProRule annotationAdd
BLAST
Domaini468 – 1187720REJPROSITE-ProRule annotationAdd
BLAST
Domaini1353 – 140351GPSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polycystin family.Curated
Contains 2 C-type lectin domains.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 1 REJ domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294369.
InParanoidiQ26627.

Family and domain databases

Gene3Di3.10.100.10. 2 hits.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR000203. GPS.
IPR002859. PKD/REJ-like.
IPR014010. REJ-like.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF01825. GPS. 1 hit.
PF00059. Lectin_C. 2 hits.
PF02010. REJ. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 2 hits.
SM00181. EGF. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50041. C_TYPE_LECTIN_2. 2 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50221. GPS. 1 hit.
PS51111. REJ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q26627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFVILILF NAILNFKTVG PAAVSSKTVD CASAPCMNGG TCADGFNNFT
60 70 80 90 100
CFCEEGFTGS MCETEYGCFR PWFYGPAFGY CYLWERVDYN WTQARESCID
110 120 130 140 150
QGRGAELASI HSAEENAFVY AQIRRYAWIG LSDQVTEGVF DYADGTPVDY
160 170 180 190 200
LSFPDKNKQS ETRDCVYVKH LRVDNWSLLD CRANKTSICK STTTFSATDG
210 220 230 240 250
CATGWVHNPA TGYCYFYEER GGMWSKGREF CLDAGADLAS IHSAEENAFI
260 270 280 290 300
FDMLTEFVWL GLNDLETEGV YTNFSDGTPA DFDNFPADNY QNEDHDCVSI
310 320 330 340 350
RHLEKTDRYW FFLGCDDTVT SICKRPHEVV ATQAPPYLFT TTDVPATTTT
360 370 380 390 400
IPTTTTTISA TTRTIDAMIL IELLLFPNNV SSIDNIFRVN NTILVTASVL
410 420 430 440 450
GSFQALSSQI LWSITNHLSG DSVQYTVYSE DAVLVRFTTV SIFTIKATAV
460 470 480 490 500
GNSHNLTATA NATVKHVCIS SLTTSVKEHC DAPHPAVYLR AFDIHISTHM
510 520 530 540 550
ELNGKCIDPM TPDFKWRIFT STEADDVVTA FEKITHTRQV MIPRGTLPYG
560 570 580 590 600
IYSLNLNAKT RLKTSGEVTG EKEIISWLEI QPPPLVAVIK GGASRSHGVS
610 620 630 640 650
SNLIVDGSNS YDPDVPPGSS SNVTFLWYCV VVDPDVMYSS LDEAIQNTDN
660 670 680 690 700
ACFEDEGIMM NSTSSMIEVI ANKLNANVTM NFWLNISKEG QISGLTQQRI
710 720 730 740 750
HLTLGLLPEI EISCISNCNM YIFTAERLVL HASCTNCDSE NEDVSFRWSL
760 770 780 790 800
ESNHTSVIGD LSSQTTTGLD QPYLVLKPLT FDSISEMGSI ILRVTGSQSD
810 820 830 840 850
SDGYAEFSVD LPHNAPPALG SCVVTPDEGY ALQTDFTVTC SNFTDVDEPL
860 870 880 890 900
TYQIILFSHV DVVDWMFVGR GEGFQLYEGS APIKDGLYLP VGVGTDDHDI
910 920 930 940 950
LLQVNVRDCN MASTSVYISA TVHPPTLDAV GMNLVQELLD MALLVETNVN
960 970 980 990 1000
ALLAVGDPGQ AAQLISALGS ILNSIGDEDD SEEGRETRSE IRSFLVDCVA
1010 1020 1030 1040 1050
AIPVESMTSL KQSSAALAVV THNKQEISTH VQMLAASTLS EMTSFVKSKS
1060 1070 1080 1090 1100
GSYTQAQENI ESAGTILVEG LSNILSAAKE TERLLSDDTS QEREDHKNLI
1110 1120 1130 1140 1150
EVAVSTINDI QDAIVAGKIP SEAATIITSP ALSIAVGSIS RDELAEATFG
1160 1170 1180 1190 1200
GPEDLGSFRM PSQDVLNQAM EHALGTTVSM KMSAMKWNPF SWPGAGGESI
1210 1220 1230 1240 1250
KSSIVGIQLE ADQMLEFHDL TADIDVYLPM RETLSADPVS VHITKTSSDS
1260 1270 1280 1290 1300
VLIDHSSLPV DGALHLTVIA ENEPMVALSI CTARISITES SCVGTDTPLG
1310 1320 1330 1340 1350
VSNEDPDTDA NFTWTVPLVD LKAADGIMIR LYDSEDQPGY ENDNITLSVF
1360 1370 1380 1390 1400
MHTLQCNFWN EDQQEWDSTG CKVGPLSKPS TTHCLCNHLT GFFGSSILVP
1410 1420 1430 1440 1450
PNHAQPVIGG HKLTGVDFLI CVLIGYGIYC VALVIRVVGC SFAIRVHKVL
Length:1,450
Mass (Da):158,165
Last modified:October 31, 1996 - v1
Checksum:i66C322FB26F918F5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti440 – 4401V → A.1 Publication
Natural varianti593 – 5931A → P.1 Publication
Natural varianti1204 – 12041I → M.1 Publication
Natural varianti1371 – 13711C → S.1 Publication
Natural varianti1390 – 13901T → A.1 Publication
Natural varianti1443 – 14431A → V.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40832 mRNA. Translation: AAB08448.1.
PIRiT30273.
RefSeqiNP_999773.1. NM_214608.1.
UniGeneiSpu.212.

Genome annotation databases

EnsemblMetazoaiSPU_023767-tr; SPU_023767-tr; SPU_023767.
GeneIDi373460.
KEGGispu:373460.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40832 mRNA. Translation: AAB08448.1.
PIRiT30273.
RefSeqiNP_999773.1. NM_214608.1.
UniGeneiSpu.212.

3D structure databases

ProteinModelPortaliQ26627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7668.Q26627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiSPU_023767-tr; SPU_023767-tr; SPU_023767.
GeneIDi373460.
KEGGispu:373460.

Organism-specific databases

CTDi373460.

Phylogenomic databases

eggNOGiNOG294369.
InParanoidiQ26627.

Family and domain databases

Gene3Di3.10.100.10. 2 hits.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR000203. GPS.
IPR002859. PKD/REJ-like.
IPR014010. REJ-like.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF01825. GPS. 1 hit.
PF00059. Lectin_C. 2 hits.
PF02010. REJ. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 2 hits.
SM00181. EGF. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50041. C_TYPE_LECTIN_2. 2 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50221. GPS. 1 hit.
PS51111. REJ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sea urchin sperm receptor for egg jelly is a modular protein with extensive homology to the human polycystic kidney disease protein, PKD1."
    Moy G.W., Mendoza L.M., Schulz J.R., Swanson W.J., Glabe C.G., Vacquier V.D.
    J. Cell Biol. 133:809-817(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANTS ALA-440; PRO-593; MET-1204; SER-1371; ALA-1390 AND VAL-1443.
    Tissue: Testis.

Entry informationi

Entry nameiSUREJ_STRPU
AccessioniPrimary (citable) accession number: Q26627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 9, 2002
Last sequence update: October 31, 1996
Last modified: March 3, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.