Retinol dehydratase - Spodoptera frugiperda (Fall armyworm)

Preferred order of sections

Names and origin

Protein names	Submitted name: Retinol dehydratase EMBL AAC47136.1
Organism	Spodoptera frugiperda (Fall armyworm) EMBL AAC47136.1
Taxonomic identifier	7108 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Noctuoidea › Noctuidae › Amphipyrinae › Spodoptera

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1X8J PDB 1X8K PDB 1FML PDB 1FMJ PDB 1X8L Metal-binding PDB 1X8J PDB 1X8K PDB 1FML PDB 1FMJ PDB 1X8L
Technical term	3D-structure PDB 1X8J PDB 1X8K PDB 1FML PDB 1FMJ PDB 1X8L
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfotransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

118

1

Calcium; via carbonyl oxygen PDB 1X8J PDB 1X8K PDB 1FMJ PDB 1X8L

Metal binding

121

1

Calcium PDB 1X8J PDB 1X8K PDB 1FMJ PDB 1X8L

Metal binding

122

1

Calcium PDB 1X8J PDB 1X8K PDB 1FMJ PDB 1X8L

Sequences

Sequence

Length

Mass (Da)

Tools

Q26490 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 004D9CE41E56FB6E
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FASTA

351

41,563

        10         20         30         40         50         60 
MEKQQDLPFP YEFRELNPEE DKLVKANLGA FPTTYVKLGP KGYMVYRPYL KDAANIYNMP 

        70         80         90        100        110        120 
LRPTDVFVAS YQRSGTTMTQ ELVWLIENDL NFEAAKTYMS LRYIYLDGFM IYDPEKQEEY 

       130        140        150        160        170        180 
NDILPNPENL DMERYLGLLE YFSRPGSSLL AAVPPTEKRF VKTHLPLSLM PPNMLDTVKM 

       190        200        210        220        230        240 
VYLARDPRDV AVSSFHHARL LYLLNKQSNF KDFWEMFHRG LYTLTPYFEH VKEAWAKRHD 

       250        260        270        280        290        300 
PNMLFLFYED YLKDLPGCIA RIADFLGKKL SEEQIQRLCE HLNFEKFKNN GAVNMEDYRE 

       310        320        330        340        350 
IGILADGEHF IRKGKAGCWR DYFDEEMTKQ AEKWIKDNLK DTDLRYPNME L

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References

[1]	"Purification, cloning, and bacterial expression of retinol dehydratase from Spodoptera frugiperda." Grun F., Noy N., Hammerling U., Buck J. J. Biol. Chem. 271:16135-16138(1996) [PubMed: 8663216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Affinity labeling of aryl sulfotransferase IV. Identification of a peptide sequence at the binding site for 3'-phosphoadenosine-5'-phosphosulfate." Zheng Y., Bergold A., Duffel M.W. J. Biol. Chem. 269:30313-30319(1994) [PubMed: 7982943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"A P-loop related motif (GxxGxxK) highly conserved in sulfotransferases is required for binding the activated sulfate donor." Komatsu K., Driscoll W.J., Koh Y., Strott C.A. Biochem. Biophys. Res. Commun. 204:1178-1185(1994) [PubMed: 7980593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"A helical lid converts a sulfotransferase to a dehydratase." Pakhomova S., Kobayashi M., Buck J., Newcomer M.E. Nat. Struct. Biol. 8:447-451(2001) [PubMed: 11323722] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH CALCIUM.
[5]	"The structures of the unique sulfotransferase retinol dehydratase with product and inhibitors provide insight into enzyme mechanism and inhibition." Pakhomova S., Buck J., Newcomer M.E. Protein Sci. 14:176-182(2005) [PubMed: 15608121] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U28654 mRNA. Translation: AAC47136.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FMJ	X-ray	2.00	A/B	1-351	[»]
1FML	X-ray	2.75	A/B	1-351	[»]
1X8J	X-ray	2.35	A/B	1-351	[»]
1X8K	X-ray	2.75	A/B	1-351	[»]
1X8L	X-ray	2.10	A/B	1-351	[»]

ProteinModelPortal

Q26490.

SMR

Q26490. Positions 8-349.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000863. Sulfotransferase_dom.
[Graphical view]

Pfam

PF00685. Sulfotransfer_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q26490_SPOFR

Accession

Primary (citable) accession number: Q26490

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	February 8, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)