UniProtKB - Q26454 (MCM4_DROME)
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Protein
DNA replication licensing factor MCM4
Gene
dpa
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. Essential role in mitotic DNA replication but not in endoreplication.2 Publications
Catalytic activityi
ATP + H2O = ADP + phosphate.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 512 – 519 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: InterPro
GO - Biological processi
- DNA replication Source: FlyBase
- DNA replication initiation Source: InterPro
- mitotic spindle organization Source: FlyBase
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-176187. Activation of ATR in response to replication stress. R-DME-68867. Assembly of the pre-replicative complex. R-DME-68949. Orc1 removal from chromatin. R-DME-68962. Activation of the pre-replicative complex. R-DME-69052. Switching of origins to a post-replicative state. R-DME-69300. Removal of licensing factors from origins. |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM4 (EC:3.6.4.12)Alternative name(s): Protein disc proliferation abnormal |
Gene namesi | Name:dpa ORF Names:CG1616 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0015929. dpa. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 518 | K → A: Slihgtly reduces complex helicase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194104 | 1 – 866 | DNA replication licensing factor MCM4Add BLAST | 866 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 55 | Phosphoserine2 Publications | 1 | |
Modified residuei | 81 | Phosphoserine1 Publication | 1 | |
Modified residuei | 87 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q26454. |
PRIDEi | Q26454. |
PTM databases
iPTMneti | Q26454. |
Expressioni
Gene expression databases
Bgeei | FBgn0015929. |
ExpressionAtlasi | Q26454. differential. |
Genevisiblei | Q26454. DM. |
Interactioni
Subunit structurei
Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication
Binary interactionsi
Protein-protein interaction databases
BioGridi | 61555. 14 interactors. |
DIPi | DIP-35346N. |
IntActi | Q26454. 11 interactors. |
STRINGi | 7227.FBpp0088055. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 460 – 669 | MCMAdd BLAST | 210 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 644 – 647 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0478. Eukaryota. COG1241. LUCA. |
GeneTreei | ENSGT00910000144163. |
InParanoidi | Q26454. |
KOi | K02212. |
OMAi | KNTIRIC. |
OrthoDBi | EOG091G02V8. |
PhylomeDBi | Q26454. |
Family and domain databases
InterProi | View protein in InterPro IPR003593. AAA+_ATPase. IPR031327. MCM. IPR008047. MCM_4. IPR018525. MCM_CS. IPR001208. MCM_dom. IPR027925. MCM_N. IPR033762. MCM_OB. IPR012340. NA-bd_OB-fold. IPR027417. P-loop_NTPase. |
PANTHERi | PTHR11630. PTHR11630. 1 hit. PTHR11630:SF66. PTHR11630:SF66. 1 hit. |
Pfami | View protein in Pfam PF00493. MCM. 1 hit. PF14551. MCM_N. 1 hit. PF17207. MCM_OB. 1 hit. |
PRINTSi | PR01657. MCMFAMILY. PR01660. MCMPROTEIN4. |
SMARTi | View protein in SMART SM00382. AAA. 1 hit. SM00350. MCM. 1 hit. |
SUPFAMi | SSF50249. SSF50249. 1 hit. SSF52540. SSF52540. 1 hit. |
PROSITEi | View protein in PROSITE PS00847. MCM_1. 1 hit. PS50051. MCM_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q26454-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSPARSPSV GGATPKQGAR TPTRGIASQD VETPMRMGPG RAVRPSDNIS
60 70 80 90 100
LPPTSPGNIS LPATSPARGL GANMSEIDLS SPLNYGTPSS MGSIRTPRSG
110 120 130 140 150
IRGTPLRARP DIRTDKRIRQ VAIGGGSGLE PIPEKGSETT DPVSESSQAP
160 170 180 190 200
QLVVWGTNVV VSQCKSKFKS FIMRFIDPSA EQDEISENID VNQPLYLQKL
210 220 230 240 250
EEIHTLEEPY LNLNCAHLKT FDQDLYRQLI CYPQEVIPGF DMAINEMFFE
260 270 280 290 300
RYPAALLEHQ IQVRPFNADK TRNMRSLNPE DMDQLISISG MVIRSSNVIP
310 320 330 340 350
EMREAFFSCN ICSFSTTVEV DRGRINQPTL CTNCNTNHCF RLIHNRSEFT
360 370 380 390 400
DKQLVKLQES PDDMAAGQTP HNVLLYAHND LVDKVQPGDR VTVTGIYRAT
410 420 430 440 450
PLKTGGLSSS VKSVYKTHVD VVHFRKVDNK RLYEDEEGKD HIFPPERVEL
460 470 480 490 500
LQLLAKKPDI YDRLARAIAP SIYENDDIKK GILLQLFGGT KKKHATLGRQ
510 520 530 540 550
NFRSEIHLLL CGDPGTSKSQ MLQYVFNLVP RSQYTSGRGS SAVGLTAYVT
560 570 580 590 600
KDPETRQLVL QTGALVLADN GVCCIDEFDK MNDSTRSVLH EVMEQQTLSI
610 620 630 640 650
AKAGIICQLN ARTSILAAAN PAESQWNKRK NIIDNVQLPH TLLSRFDLIF
660 670 680 690 700
LVLDPQDEIF DKRLASHLVS LYYVTRHEEE DTMFDMSVLR DYIAYAREHL
710 720 730 740 750
SPTLSDEAQQ RLIQAYVDMR KVGAGRGQIS AYPRQLESLI RLSEAHAKVR
760 770 780 790 800
LSNQVELLDV EEAWRLHREA LKQSATDPLS GKIDVGILTT GLSTAARKKR
810 820 830 840 850
ADLVAAIKEN LKKKGKVLTV PYQKLFSDIK EGSQIMITRE QFEDALKEVQ
860
DEGAIVVMGK NTIRIC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 435 | D → E in AAB35644 (PubMed:7489728).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S80255 mRNA. Translation: AAB35644.1. AE013599 Genomic DNA. Translation: AAF59242.1. |
PIRi | S59872. |
RefSeqi | NP_001286160.1. NM_001299231.1. NP_477185.1. NM_057837.5. |
UniGenei | Dm.21562. |
Genome annotation databases
EnsemblMetazoai | FBtr0088982; FBpp0088055; FBgn0015929. FBtr0345797; FBpp0311783; FBgn0015929. |
GeneIDi | 35679. |
KEGGi | dme:Dmel_CG1616. |
Similar proteinsi
Entry informationi
Entry namei | MCM4_DROME | |
Accessioni | Q26454Primary (citable) accession number: Q26454 Secondary accession number(s): Q9V4M5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 2, 2001 | |
Last modified: | March 28, 2018 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |