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UniProtKB - Q26454 (MCM4_DROME)

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Protein

DNA replication licensing factor MCM4

Gene

dpa

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. Essential role in mitotic DNA replication but not in endoreplication.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi512 – 519ATPSequence analysis8

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

  • DNA replication Source: FlyBase
  • DNA replication initiation Source: InterPro
  • mitotic spindle organization Source: FlyBase
Complete GO annotation...

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM4 (EC:3.6.4.12)
Alternative name(s):
Protein disc proliferation abnormal
Gene namesi
Name:dpa
ORF Names:CG1616
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora ›
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015929. dpa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi518K → A: Slihgtly reduces complex helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941041 – 866DNA replication licensing factor MCM4Add BLAST866

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55Phosphoserine2 Publications1
Modified residuei81Phosphoserine1 Publication1
Modified residuei87Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ26454.
PRIDEiQ26454.

PTM databases

iPTMnetiQ26454.

Expressioni

Gene expression databases

BgeeiFBgn0015929.
ExpressionAtlasiQ26454. differential.
GenevisibleiQ26454. DM.

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mcm6Q9V4613EBI-175772,EBI-869161

Protein-protein interaction databases

BioGridi61555. 7 interactors.
DIPiDIP-35346N.
IntActiQ26454. 8 interactors.
STRINGi7227.FBpp0088055.

Structurei

3D structure databases

ProteinModelPortaliQ26454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini460 – 669MCMAdd BLAST210

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi644 – 647Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0478. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00880000137967.
InParanoidiQ26454.
KOiK02212.
OMAiKNTIRIC.
OrthoDBiEOG091G02V8.
PhylomeDBiQ26454.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008047. MCM_4.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01660. MCMPROTEIN4.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q26454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPARSPSV GGATPKQGAR TPTRGIASQD VETPMRMGPG RAVRPSDNIS 
        60         70         80         90        100
LPPTSPGNIS LPATSPARGL GANMSEIDLS SPLNYGTPSS MGSIRTPRSG 
       110        120        130        140        150
IRGTPLRARP DIRTDKRIRQ VAIGGGSGLE PIPEKGSETT DPVSESSQAP 
       160        170        180        190        200
QLVVWGTNVV VSQCKSKFKS FIMRFIDPSA EQDEISENID VNQPLYLQKL 
       210        220        230        240        250
EEIHTLEEPY LNLNCAHLKT FDQDLYRQLI CYPQEVIPGF DMAINEMFFE 
       260        270        280        290        300
RYPAALLEHQ IQVRPFNADK TRNMRSLNPE DMDQLISISG MVIRSSNVIP 
       310        320        330        340        350
EMREAFFSCN ICSFSTTVEV DRGRINQPTL CTNCNTNHCF RLIHNRSEFT 
       360        370        380        390        400
DKQLVKLQES PDDMAAGQTP HNVLLYAHND LVDKVQPGDR VTVTGIYRAT 
       410        420        430        440        450
PLKTGGLSSS VKSVYKTHVD VVHFRKVDNK RLYEDEEGKD HIFPPERVEL 
       460        470        480        490        500
LQLLAKKPDI YDRLARAIAP SIYENDDIKK GILLQLFGGT KKKHATLGRQ 
       510        520        530        540        550
NFRSEIHLLL CGDPGTSKSQ MLQYVFNLVP RSQYTSGRGS SAVGLTAYVT 
       560        570        580        590        600
KDPETRQLVL QTGALVLADN GVCCIDEFDK MNDSTRSVLH EVMEQQTLSI 
       610        620        630        640        650
AKAGIICQLN ARTSILAAAN PAESQWNKRK NIIDNVQLPH TLLSRFDLIF 
       660        670        680        690        700
LVLDPQDEIF DKRLASHLVS LYYVTRHEEE DTMFDMSVLR DYIAYAREHL 
       710        720        730        740        750
SPTLSDEAQQ RLIQAYVDMR KVGAGRGQIS AYPRQLESLI RLSEAHAKVR 
       760        770        780        790        800
LSNQVELLDV EEAWRLHREA LKQSATDPLS GKIDVGILTT GLSTAARKKR 
       810        820        830        840        850
ADLVAAIKEN LKKKGKVLTV PYQKLFSDIK EGSQIMITRE QFEDALKEVQ 
       860 
DEGAIVVMGK NTIRIC
Length:866
Mass (Da):96,610
Last modified:November 2, 2001 - v2
Checksum:iCF6ED69D2ADDA04E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti435D → E in AAB35644 (PubMed:7489728).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80255 mRNA. Translation: AAB35644.1.
AE013599 Genomic DNA. Translation: AAF59242.1.
PIRiS59872.
RefSeqiNP_001286160.1. NM_001299231.1.
NP_477185.1. NM_057837.5.
UniGeneiDm.21562.

Genome annotation databases

EnsemblMetazoaiFBtr0088982; FBpp0088055; FBgn0015929.
FBtr0345797; FBpp0311783; FBgn0015929.
GeneIDi35679.
KEGGidme:Dmel_CG1616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80255 mRNA. Translation: AAB35644.1.
AE013599 Genomic DNA. Translation: AAF59242.1.
PIRiS59872.
RefSeqiNP_001286160.1. NM_001299231.1.
NP_477185.1. NM_057837.5.
UniGeneiDm.21562.

3D structure databases

ProteinModelPortaliQ26454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61555. 7 interactors.
DIPiDIP-35346N.
IntActiQ26454. 8 interactors.
STRINGi7227.FBpp0088055.

PTM databases

iPTMnetiQ26454.

Proteomic databases

PaxDbiQ26454.
PRIDEiQ26454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088982; FBpp0088055; FBgn0015929.
FBtr0345797; FBpp0311783; FBgn0015929.
GeneIDi35679.
KEGGidme:Dmel_CG1616.

Organism-specific databases

CTDi103997.
FlyBaseiFBgn0015929. dpa.

Phylogenomic databases

eggNOGiKOG0478. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00880000137967.
InParanoidiQ26454.
KOiK02212.
OMAiKNTIRIC.
OrthoDBiEOG091G02V8.
PhylomeDBiQ26454.

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Miscellaneous databases

GenomeRNAii35679.
PROiPR:Q26454.

Gene expression databases

BgeeiFBgn0015929.
ExpressionAtlasiQ26454. differential.
GenevisibleiQ26454. DM.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008047. MCM_4.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01660. MCMPROTEIN4.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMCM4_DROME
AccessioniPrimary (citable) accession number: Q26454
Secondary accession number(s): Q9V4M5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2001
Last modified: June 7, 2017
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.