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Protein

DNA replication licensing factor MCM4

Gene

dpa

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. Essential role in mitotic DNA replication but not in endoreplication.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi512 – 519ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: FlyBase
  • DNA replication initiation Source: InterPro
  • mitotic spindle organization Source: FlyBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-176187 Activation of ATR in response to replication stress
R-DME-68867 Assembly of the pre-replicative complex
R-DME-68949 Orc1 removal from chromatin
R-DME-68962 Activation of the pre-replicative complex
R-DME-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM4 (EC:3.6.4.12)
Alternative name(s):
Protein disc proliferation abnormal
Gene namesi
Name:dpa
ORF Names:CG1616
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015929 dpa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi518K → A: Slihgtly reduces complex helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941041 – 866DNA replication licensing factor MCM4Add BLAST866

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55Phosphoserine2 Publications1
Modified residuei81Phosphoserine1 Publication1
Modified residuei87Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ26454
PRIDEiQ26454

PTM databases

iPTMnetiQ26454

Expressioni

Gene expression databases

BgeeiFBgn0015929
ExpressionAtlasiQ26454 differential
GenevisibleiQ26454 DM

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mcm6Q9V4614EBI-175772,EBI-869161

Protein-protein interaction databases

BioGridi61555, 14 interactors
ComplexPortaliCPX-2942 MCM complex
DIPiDIP-35346N
IntActiQ26454, 11 interactors
STRINGi7227.FBpp0088055

Structurei

3D structure databases

ProteinModelPortaliQ26454
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini460 – 669MCMAdd BLAST210

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi644 – 647Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0478 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00910000144163
InParanoidiQ26454
KOiK02212
OMAiKNTIRIC
OrthoDBiEOG091G02V8
PhylomeDBiQ26454

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008047 MCM_4
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF66 PTHR11630:SF66, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01660 MCMPROTEIN4
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q26454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPARSPSV GGATPKQGAR TPTRGIASQD VETPMRMGPG RAVRPSDNIS
60 70 80 90 100
LPPTSPGNIS LPATSPARGL GANMSEIDLS SPLNYGTPSS MGSIRTPRSG
110 120 130 140 150
IRGTPLRARP DIRTDKRIRQ VAIGGGSGLE PIPEKGSETT DPVSESSQAP
160 170 180 190 200
QLVVWGTNVV VSQCKSKFKS FIMRFIDPSA EQDEISENID VNQPLYLQKL
210 220 230 240 250
EEIHTLEEPY LNLNCAHLKT FDQDLYRQLI CYPQEVIPGF DMAINEMFFE
260 270 280 290 300
RYPAALLEHQ IQVRPFNADK TRNMRSLNPE DMDQLISISG MVIRSSNVIP
310 320 330 340 350
EMREAFFSCN ICSFSTTVEV DRGRINQPTL CTNCNTNHCF RLIHNRSEFT
360 370 380 390 400
DKQLVKLQES PDDMAAGQTP HNVLLYAHND LVDKVQPGDR VTVTGIYRAT
410 420 430 440 450
PLKTGGLSSS VKSVYKTHVD VVHFRKVDNK RLYEDEEGKD HIFPPERVEL
460 470 480 490 500
LQLLAKKPDI YDRLARAIAP SIYENDDIKK GILLQLFGGT KKKHATLGRQ
510 520 530 540 550
NFRSEIHLLL CGDPGTSKSQ MLQYVFNLVP RSQYTSGRGS SAVGLTAYVT
560 570 580 590 600
KDPETRQLVL QTGALVLADN GVCCIDEFDK MNDSTRSVLH EVMEQQTLSI
610 620 630 640 650
AKAGIICQLN ARTSILAAAN PAESQWNKRK NIIDNVQLPH TLLSRFDLIF
660 670 680 690 700
LVLDPQDEIF DKRLASHLVS LYYVTRHEEE DTMFDMSVLR DYIAYAREHL
710 720 730 740 750
SPTLSDEAQQ RLIQAYVDMR KVGAGRGQIS AYPRQLESLI RLSEAHAKVR
760 770 780 790 800
LSNQVELLDV EEAWRLHREA LKQSATDPLS GKIDVGILTT GLSTAARKKR
810 820 830 840 850
ADLVAAIKEN LKKKGKVLTV PYQKLFSDIK EGSQIMITRE QFEDALKEVQ
860
DEGAIVVMGK NTIRIC
Length:866
Mass (Da):96,610
Last modified:November 2, 2001 - v2
Checksum:iCF6ED69D2ADDA04E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti435D → E in AAB35644 (PubMed:7489728).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80255 mRNA Translation: AAB35644.1
AE013599 Genomic DNA Translation: AAF59242.1
PIRiS59872
RefSeqiNP_001286160.1, NM_001299231.1
NP_477185.1, NM_057837.5
UniGeneiDm.21562

Genome annotation databases

EnsemblMetazoaiFBtr0088982; FBpp0088055; FBgn0015929
FBtr0345797; FBpp0311783; FBgn0015929
GeneIDi35679
KEGGidme:Dmel_CG1616

Similar proteinsi

Entry informationi

Entry nameiMCM4_DROME
AccessioniPrimary (citable) accession number: Q26454
Secondary accession number(s): Q9V4M5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2001
Last modified: June 20, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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