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Protein

Mu-conotoxin MrVIB

Gene
N/A
Organism
Conus marmoreus (Marble cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mu-conotoxins block voltage-gated sodium channels. Has a preference for Nav1.4/SCN4A over Nav1.2/SCN2A sodium channels. Blocks Nav channels by interacting mainly with the C-terminal part of the pore loop of domain-3. Also blocks fast-inactivating calcium current. Blocks Nav1.8/SCN10A sodium channels and has potent and long-lasting local anesthetic effects. Can also block propagation of action potentials in A- and C-fibers in sciatic nerve as well as skeletal muscle in isolated preparations.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Mu-conotoxin MrVIB
Alternative name(s):
CGX-1002
OrganismiConus marmoreus (Marble cone)
Taxonomic identifieri42752 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri597. MrVIB precursor.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Is under preclinical trial by Cognetix Inc under the name CGX-1002 as a local anesthetic agent.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000003490223 – 49Add BLAST27
PeptideiPRO_000003490352 – 82Mu-conotoxin MrVIBAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 711 Publication
Disulfide bondi60 ↔ 761 Publication
Disulfide bondi70 ↔ 811 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi69 – 71Combined sources3
Beta strandi78 – 82Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RMKNMR-A52-82[»]
ProteinModelPortaliQ26443.
SMRiQ26443.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ26443.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
The cysteine framework is VI/VII (C-C-CC-C-C).

Sequence similaritiesi

Belongs to the conotoxin O1 superfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR004214. Conotoxin.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q26443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLTCMMIVA VLFLTAWTLV MADDSNNGLA NHFLKSRDEM EDPEASKLEK
60 70 80
RACSKKWEYC IVPILGFVYC CPGLICGPFV CV
Length:82
Mass (Da):9,210
Last modified:November 1, 1996 - v1
Checksum:iB617F8652A48F5D6
GO

Mass spectrometryi

Molecular mass is 3404.8 Da from positions 52 - 82. Determined by LSI. 1 Publication
Molecular mass is 3404.9 Da from positions 52 - 82. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78990 mRNA. Translation: AAB34916.1.
PIRiB58586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78990 mRNA. Translation: AAB34916.1.
PIRiB58586.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RMKNMR-A52-82[»]
ProteinModelPortaliQ26443.
SMRiQ26443.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri597. MrVIB precursor.

Miscellaneous databases

EvolutionaryTraceiQ26443.

Family and domain databases

InterProiIPR004214. Conotoxin.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCO16B_CONMR
AccessioniPrimary (citable) accession number: Q26443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Pharmaceutical

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.