Reviewed, UniProtKB/Swiss-Prot Q26377 (CORZ_DROME)

Names and origin

Protein names

Recommended name:
    Pro-corazonin
      Short name=Crz
      Short name=Dm-Crz
Cleaved into the following 2 chains:
    1- Recommended name:
            Corazonin
    2- Recommended name:
            Corazonin precursor-related peptide
                Short name=CPRP
        Alternative name(s):
            Crz-associated peptide

Gene names

Name:	Crz
ORF Names:	CG3302

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cardioactive peptide. Corazonin is probably involved in the physiological regulation of the heart beat. Clock (Clk) and cycle (cyc) proteins negatively regulate Crz transcription in a cell-specific manner. Ref.6
Subcellular location	Corazonin: Secreted. Corazonin precursor-related peptide: Secreted By similarity.
Tissue specificity	From late embryo to larva, expression is consistently detected in three neuronal groups: dorso-lateral neurons (DL), dorso-medial neurons (DM), and neurons in the ventral nerve cord (vCrz). Both the vCrz and DM groups die via programmed cell death during metamorphosis, whereas the DL neurons persist to adulthood. In adults, expression is seen in a cluster of six to eight neurons per lobe in the pars lateralis (DLP), in numerous neuronal cells in the optic lobes, and in a novel group of four abdominal ganglionic neurons present only in males (ms-aCrz). Projections of the ms-aCrz neurons terminate within the ventral nerve cord, implying a role as interneurons. Terminals of the DLP neurons are found in the retrocerebral complex that produces juvenile hormone and adipokinetic hormone, located in the vicinity of terminals emanating from PDF-containing pacemaking neurons. Ref.6 Ref.7
Sequence similarities	Belongs to the corazonin family.
Mass spectrometry	Molecular mass is 1369.63 Da from positions 20 - 30. Determined by MALDI. Ref.5
Sequence caution	The sequence AAB32283.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	neuropeptide signaling pathway Traceable author statement. Source: FlyBase positive regulation of heart contraction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	neuropeptide hormone activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.4
	Chain	20 – 154	135	Pro-corazonin Potential		PRO_0000341610
	Peptide	20 – 30	11	Corazonin		PRO_0000000948
	Peptide	34 – 67	34	Corazonin precursor-related peptide		PRO_0000000949
	Propeptide	70 – 154	85			PRO_0000000950
Amino acid modifications
	Modified residue	20	1	Pyrrolidone carboxylic acid
	Modified residue	30	1	Asparagine amide
Experimental info
	Sequence conflict	81	1	L → Q in AAB32283. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q26377-1 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 06046282A1C61A2F	FASTA	154	17,154
	10 20 30 40 50 60 MLRLLLLPLF LFTLSMCMGQ TFQYSRGWTN GKRSFNAASP LLANGHLHRA SELGLTDLYD 70 80 90 100 110 120 LQDWSSDRRL ERCLSQLQRS LIARNCVPGS DFNANRVDPD PENSAHPRLS NSNGENVLYS 130 140 150 SANIPNRHRQ SNELLEELSA AGGASAEPNV FGKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"Isolation and structure of the Drosophila corazonin gene." Veenstra J.A. Biochem. Biophys. Res. Commun. 204:292-296(1994) [PubMed: 7945373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
[4]	"Peptidomics of the larval Drosophila melanogaster central nervous system." Baggerman G., Cerstiaens A., De Loof A., Schoofs L. J. Biol. Chem. 277:40368-40374(2002) [PubMed: 12171930] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-30, AMIDATION. Tissue: Larva.
[5]	"Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster: a mass spectrometric survey of peptides from individual flies." Predel R., Wegener C., Russell W.K., Tichy S.E., Russell D.H., Nachman R.J. J. Comp. Neurol. 474:379-392(2004) [PubMed: 15174081] [Abstract] Cited for: MASS SPECTROMETRY. Tissue: Cerebral ganglion and Corpora cardiaca.
[6]	"Comparative analysis of Corazonin-encoding genes (Crz's) in Drosophila species and functional insights into Crz-expressing neurons." Choi Y.J., Lee G., Hall J.C., Park J.H. J. Comp. Neurol. 482:372-385(2005) [PubMed: 15669053] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY. Tissue: Head.
[7]	"Developmental regulation and functions of the expression of the neuropeptide corazonin in Drosophila melanogaster." Lee G., Kim K.-M., Kikuno K., Wang Z., Choi Y.-J., Park J.H. Cell Tissue Res. 331:659-673(2008) [PubMed: 18087727] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAF55046.1. S74038 Genomic DNA. Translation: AAB32283.1. Sequence problems.
PIR	JC2384.
RefSeq	NP_524350.1.
UniGene	Dm.2429
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0013767. Drosophila melanogaster. [Contig view]
GeneID	41742.
KEGG	dme:Dmel_CG3302.
NMPDR	fig|7227.3.peg.12748.
Organism-specific databases
FlyBase	FBgn0013767. Crz.
Phylogenomic databases
HOGENOM	Q26377.
OMA	Q26377. GQTFQYS.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-011577-MON.
Gene expression databases
GermOnline	CG3302. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	825345.

Entry information

Entry name

CORZ_DROME

Accession

Primary (citable) accession number: Q26377
Secondary accession number(s): Q9VFK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 10, 2003
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project