Reviewed, UniProtKB/Swiss-Prot Q26304 (LUCI_LUCMI)

Names and origin

Protein names	Recommended name:     Luciferin 4-monooxygenase       Short name=Luciferase     EC=1.13.12.7
Organism	Luciola mingrelica (Southern Russian firefly)
Taxonomic identifier	27446 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Elateriformia › Elateroidea › Lampyridae › Luciolinae › Luciola

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Produces green light with a wavelength of 570 nm. Ref.2
Catalytic activity	Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light. Ref.2
Cofactor	Magnesium. Ref.2 Phospholipids. Ref.2
Enzyme regulation	Inhibited by ATP analogs and sodium deoxycholate. Ref.2
Subunit structure	Homodimer. Ref.3
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
biophysicochemical properties	Kinetic parameters: KM=11.5 µM for luciferase Ref.2 KM=130 µM for ATP Ref.2 pH dependence: Optimum pH is 7.7. Ref.2

Ontologies

Keywords
Biological process	Luminescence
Cellular component	Peroxisome
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Ref.2 Inferred from direct assay. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity Ref.2 Inferred from direct assay. Source: UniProtKB magnesium ion binding Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Luciferin 4-monooxygenase		PRO_0000193145
Regions
	Motif	546 – 548	3	Microbody targeting signal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q26304-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 62C8953BDFFBC423	FASTA	548	60,495
	10 20 30 40 50 60 MEMEKEENVV YGPLPFYPIE EGSAGIQLHK YMHQYAKLGA IAFSNALTGV DISYQEYFDI 70 80 90 100 110 120 TCRLAEAMKN FGMKPEEHIA LCSENCEEFF IPVLAGLYIG VAVAPTNEIY TLRELNHSLG 130 140 150 160 170 180 IAQPTIVFSS RKGLPKVLEV QKTVTCIKKI VILDSKVNFG GHDCMETFIK KHVELGFQPS 190 200 210 220 230 240 SFVPIDVKNR KQHVALLMNS SGSTGLPKGV RITHEGAVTR FSHAKDPIYG NQVSPGTAIL 250 260 270 280 290 300 TVVPFHHGFG MFTTLGYFAC GYRVVMLTKF DEELFLRTLQ DYKCTSVILV PTLFAILNKS 310 320 330 340 350 360 ELIDKFDLSN LTEIASGGAP LAKEVGEAVA RRFNLPGVRQ GYGLTETTSA FIITPEGDDK 370 380 390 400 410 420 PGASGKVVPL FKVKVIDLDT KKTLGVNRRG EICVKGPSLM LGYSNNPEAT RETIDEEGWL 430 440 450 460 470 480 HTGDIGYYDE DEHFFIVDRL KSLIKYKGYQ VPPAELESVL LQHPNIFDAG VAGVPDPDAG 490 500 510 520 530 540 ELPGAVVVME KGKTMTEKEI VDYVNSQVVN HKRLRGGVRF VDEVPKGLTG KIDAKVIREI LKKPQAKM

References

[1]	"Luciferase from the east European firefly Luciola mingrelica: cloning and nucleotide sequence of the cDNA, overexpression in Escherichia coli and purification of the enzyme." Devine J.H., Kutuzova G.D., Green V.A., Ugarova N.N., Baldwin T.O. Biochim. Biophys. Acta 1173:121-132(1993) [PubMed: 8504162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Luciferase of Luciola mingrelica fireflies. Kinetics and regulation mechanism." Ugarova N.N. J. Biolumin. Chemilumin. 4:406-418(1989) [PubMed: 2801227] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[3]	"Dimers as catalytically active particles of firefly luciferase." Ugarova N.N., Brovko L. Jr., Belyayeva E.I., Philippova N. Jr., Berezin I.V. Dokl. Akad. Nauk SSSR 260:358-360(1981) Cited for: SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S61961 mRNA. Translation: AAB26932.1.
PIR	S33788.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
SMR	Q26304. Positions 6-544.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.12.7. 229293.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUCI_LUCMI

Accession

Primary (citable) accession number: Q26304

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)