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Q26255 (PUR2_CHITE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trifunctional purine biosynthetic protein adenosine-3

Including the following 3 domains:

  1. Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name=GARS
    Phosphoribosylglycinamide synthetase
  2. Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
    Alternative name(s):
    AIR synthase
    Short name=AIRS
    Phosphoribosyl-aminoimidazole synthetase
  3. Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name=GART
Gene names
Name:GART
OrganismChironomus tentans (Midge) (Camptochironomus tentans)
Taxonomic identifier7153 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraChironomoideaChironomidaeChironominaeChironomus

Protein attributes

Sequence length1371 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00138

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00138

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the central section; belongs to the AIR synthase family.

In the C-terminal section; belongs to the GART family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13711371Trifunctional purine biosynthetic protein adenosine-3 HAMAP-Rule MF_00138
PRO_0000074933

Regions

Domain115 – 321207ATP-grasp
Nucleotide binding141 – 20262ATP By similarity
Region434 – 1171738AIRS HAMAP-Rule MF_00138
Region1172 – 1371200GART HAMAP-Rule MF_00138
Region1180 – 118235'-phosphoribosylglycinamide binding By similarity
Region1260 – 1263410-formyltetrahydrofolate binding By similarity
Region1311 – 1315510-formyltetrahydrofolate binding By similarity
Region1341 – 134445'-phosphoribosylglycinamide binding By similarity

Sites

Active site12791Proton donor By similarity
Metal binding2911Manganese By similarity
Metal binding2931Manganese By similarity
Binding site1235110-formyltetrahydrofolate By similarity
Binding site1277110-formyltetrahydrofolate By similarity
Site13151Raises pKa of active site His By similarity

Sequences

Sequence LengthMass (Da)Tools
Q26255 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7BF4664DB1538946

FASTA1,371149,103
        10         20         30         40         50         60 
MTGKKLLLIG SGGREHALAW KLQQSKNVTE IFAFPGSIGI SQLEKVQLVN NNEMNLKDFK 

        70         80         90        100        110        120 
GIASWCKINH IDLVIVGPED PLAEGIADQL KAANIHCFGP SKAGARIESD KSWSKDFMIR 

       130        140        150        160        170        180 
HHIPTAQYGS FIDALKAKDF IRNTPNALVV KASGLAAGKG VIVAENIEEA CAAVDEILGD 

       190        200        210        220        230        240 
HKFGTAGDVV VVEEKLSGQE VSVLGFVDSN SVRILPPAQD HKRLKDNDEG LNTGGMGAYC 

       250        260        270        280        290        300 
PCPLISQQEL DIVKSQVLQR AVDGLRKENI LYNGILYAGI MLTHDGPKTL EFNCRFGDPE 

       310        320        330        340        350        360 
TQIILPLLDE DLYDLMMASC TNHLCNVPEL KFKSNINAVG VVMASKGYPE TSTKGCVISG 

       370        380        390        400        410        420 
IESVETMDNH IVFHSGTSKN NKDEWITNGG RVLINIALAD NLKKAADLAT KACDVVKFDG 

       430        440        450        460        470        480 
SQYRRDIGKK AFQIHSLTYK ESGVNIEAGN SLVGRIKSLS YGTHRSGVVG QIGSFGGLMR 

       490        500        510        520        530        540 
LNDIKYINSN GEESNYKDIV LVQGTDGVGT KLKIAESMNV WDTIGIDLVA MCVNDVLCNG 

       550        560        570        580        590        600 
AEPIGFLDYI ACGHLEVPTV ATIVKGIADG CRKANCALIG GETAEMPSMY GKGKYDLAGY 

       610        620        630        640        650        660 
CVGITEYDEI LPKINDVHVG DVVIGLPSSG IHSNGFSLVN KIFQQTGFKL TDIAEFSDSH 

       670        680        690        700        710        720 
KSYGMEFLTP TRLYVSETLP FLRNGYVKAL AHITGGGLLE NIPRILPNHL SVQIDALTWK 

       730        740        750        760        770        780 
LPKVFSWLAA HGNVNANEML RTFNCGIGMI IIMPRNDIEW ETIPEARMIG SITQRDHNGP 

       790        800        810        820        830        840 
QVIVKNFKEV LHKEVTHWKK GDAETTSISY KDSGVDITAG NELVDNIKPH AKSTNRKGVI 

       850        860        870        880        890        900 
GGLGSFGGLF RINECGTKFE DPMLVLATDG VGTKLKIAQQ LGKHDTVGID LVAMCNNDIL 

       910        920        930        940        950        960 
CNGAEPLTFL DYFACGKLDV NVATNVVSGI AEGCRQSDST LLGGETAEMP GMYNPNVYDL 

       970        980        990       1000       1010       1020 
AGFSLGVAEH EDILPKKNCL EVGDIIIGFP SNGVHSNGFS LIHKLFELTG YKWTDIAPFS 

      1030       1040       1050       1060       1070       1080 
AYGKTFGEEF LEPTKVYVKE ISPALKTGYV KALAHITGGG LWDNIPRVLP YNLTAELDAK 

      1090       1100       1110       1120       1130       1140 
KINISPVFAW LSLNGNIDKL ELLKTFNCGI GMIMIASKEH ELEILKSLYG SRASVIGKII 

      1150       1160       1170       1180       1190       1200 
PTKPHGHQVI VRHFATCFER VERLLSIPKK RVGVLISGSG SNLQALIDAT KSTNMGMCSE 

      1210       1220       1230       1240       1250       1260 
IVFVLSNKAG IFGLERAAKA NIPSTVISNK DYATREAFDV ALHNELIKHN VEIICLAGFM 

      1270       1280       1290       1300       1310       1320 
RILTPCFVNK WKGKLLNIHP SLLPKYKGIT AQKDALESGD NESGCTVHFV DENVDTGAII 

      1330       1340       1350       1360       1370 
VQEIVPIFEN DTVESLTERI HVAEHIAFPK ALRLVASGYV RLNDKCETEW A 

« Hide

References

[1]"Unusual organizational features of the Drosophila Gart locus are not conserved within Diptera."
Clark D.V., Henikoff S.
J. Mol. Evol. 35:51-59(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S43653 Genomic DNA. Translation: AAB23115.1.

3D structure databases

ProteinModelPortalQ26255.
SMRQ26255. Positions 494-792.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Family and domain databases

Gene3D3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPMF_00138. GARS.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_CHITE
AccessionPrimary (citable) accession number: Q26255
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways