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Q26255

- PUR2_CHITE

UniProt

Q26255 - PUR2_CHITE

Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Chironomus tentans (Midge) (Camptochironomus tentans)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
    10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi291 – 2911ManganeseBy similarity
    Metal bindingi293 – 2931ManganeseBy similarity
    Binding sitei1235 – 1235110-formyltetrahydrofolateBy similarity
    Binding sitei1277 – 1277110-formyltetrahydrofolateBy similarity
    Active sitei1279 – 12791Proton donorBy similarity
    Sitei1315 – 13151Raises pKa of active site HisBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi141 – 20262ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. methyltransferase activity Source: InterPro
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
    5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
    6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00125.
    UPA00074; UER00126.
    UPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trifunctional purine biosynthetic protein adenosine-3
    Including the following 3 domains:
    Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name:
    GART
    Gene namesi
    Name:GART
    OrganismiChironomus tentans (Midge) (Camptochironomus tentans)
    Taxonomic identifieri7153 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraChironomoideaChironomidaeChironominaeChironomus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13711371Trifunctional purine biosynthetic protein adenosine-3PRO_0000074933Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ26255.
    SMRiQ26255. Positions 494-792.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini115 – 321207ATP-graspAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni434 – 1171738AIRSAdd
    BLAST
    Regioni1172 – 1371200GARTAdd
    BLAST
    Regioni1180 – 118235'-phosphoribosylglycinamide bindingBy similarity
    Regioni1260 – 1263410-formyltetrahydrofolate bindingBy similarity
    Regioni1311 – 1315510-formyltetrahydrofolate bindingBy similarity
    Regioni1341 – 134445'-phosphoribosylglycinamide bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the GARS family.Curated
    In the central section; belongs to the AIR synthase family.Curated
    In the C-terminal section; belongs to the GART family.Curated
    Contains 1 ATP-grasp domain.Curated

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 2 hits.
    HAMAPiMF_00138. GARS.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    TIGR00878. purM. 2 hits.
    TIGR00639. PurN. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q26255-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGKKLLLIG SGGREHALAW KLQQSKNVTE IFAFPGSIGI SQLEKVQLVN     50
    NNEMNLKDFK GIASWCKINH IDLVIVGPED PLAEGIADQL KAANIHCFGP 100
    SKAGARIESD KSWSKDFMIR HHIPTAQYGS FIDALKAKDF IRNTPNALVV 150
    KASGLAAGKG VIVAENIEEA CAAVDEILGD HKFGTAGDVV VVEEKLSGQE 200
    VSVLGFVDSN SVRILPPAQD HKRLKDNDEG LNTGGMGAYC PCPLISQQEL 250
    DIVKSQVLQR AVDGLRKENI LYNGILYAGI MLTHDGPKTL EFNCRFGDPE 300
    TQIILPLLDE DLYDLMMASC TNHLCNVPEL KFKSNINAVG VVMASKGYPE 350
    TSTKGCVISG IESVETMDNH IVFHSGTSKN NKDEWITNGG RVLINIALAD 400
    NLKKAADLAT KACDVVKFDG SQYRRDIGKK AFQIHSLTYK ESGVNIEAGN 450
    SLVGRIKSLS YGTHRSGVVG QIGSFGGLMR LNDIKYINSN GEESNYKDIV 500
    LVQGTDGVGT KLKIAESMNV WDTIGIDLVA MCVNDVLCNG AEPIGFLDYI 550
    ACGHLEVPTV ATIVKGIADG CRKANCALIG GETAEMPSMY GKGKYDLAGY 600
    CVGITEYDEI LPKINDVHVG DVVIGLPSSG IHSNGFSLVN KIFQQTGFKL 650
    TDIAEFSDSH KSYGMEFLTP TRLYVSETLP FLRNGYVKAL AHITGGGLLE 700
    NIPRILPNHL SVQIDALTWK LPKVFSWLAA HGNVNANEML RTFNCGIGMI 750
    IIMPRNDIEW ETIPEARMIG SITQRDHNGP QVIVKNFKEV LHKEVTHWKK 800
    GDAETTSISY KDSGVDITAG NELVDNIKPH AKSTNRKGVI GGLGSFGGLF 850
    RINECGTKFE DPMLVLATDG VGTKLKIAQQ LGKHDTVGID LVAMCNNDIL 900
    CNGAEPLTFL DYFACGKLDV NVATNVVSGI AEGCRQSDST LLGGETAEMP 950
    GMYNPNVYDL AGFSLGVAEH EDILPKKNCL EVGDIIIGFP SNGVHSNGFS 1000
    LIHKLFELTG YKWTDIAPFS AYGKTFGEEF LEPTKVYVKE ISPALKTGYV 1050
    KALAHITGGG LWDNIPRVLP YNLTAELDAK KINISPVFAW LSLNGNIDKL 1100
    ELLKTFNCGI GMIMIASKEH ELEILKSLYG SRASVIGKII PTKPHGHQVI 1150
    VRHFATCFER VERLLSIPKK RVGVLISGSG SNLQALIDAT KSTNMGMCSE 1200
    IVFVLSNKAG IFGLERAAKA NIPSTVISNK DYATREAFDV ALHNELIKHN 1250
    VEIICLAGFM RILTPCFVNK WKGKLLNIHP SLLPKYKGIT AQKDALESGD 1300
    NESGCTVHFV DENVDTGAII VQEIVPIFEN DTVESLTERI HVAEHIAFPK 1350
    ALRLVASGYV RLNDKCETEW A 1371
    Length:1,371
    Mass (Da):149,103
    Last modified:November 1, 1996 - v1
    Checksum:i7BF4664DB1538946
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S43653 Genomic DNA. Translation: AAB23115.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S43653 Genomic DNA. Translation: AAB23115.1 .

    3D structure databases

    ProteinModelPortali Q26255.
    SMRi Q26255. Positions 494-792.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    UPA00074 ; UER00126 .
    UPA00074 ; UER00129 .

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 2 hits.
    HAMAPi MF_00138. GARS.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    TIGR00878. purM. 2 hits.
    TIGR00639. PurN. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Unusual organizational features of the Drosophila Gart locus are not conserved within Diptera."
      Clark D.V., Henikoff S.
      J. Mol. Evol. 35:51-59(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPUR2_CHITE
    AccessioniPrimary (citable) accession number: Q26255
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3