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Q26255

- PUR2_CHITE

UniProt

Q26255 - PUR2_CHITE

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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Chironomus tentans (Midge) (Camptochironomus tentans)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi291 – 2911ManganeseBy similarity
Metal bindingi293 – 2931ManganeseBy similarity
Binding sitei1235 – 1235110-formyltetrahydrofolateBy similarity
Binding sitei1277 – 1277110-formyltetrahydrofolateBy similarity
Active sitei1279 – 12791Proton donorBy similarity
Sitei1315 – 13151Raises pKa of active site HisBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi141 – 20262ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
  6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiChironomus tentans (Midge) (Camptochironomus tentans)
Taxonomic identifieri7153 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraChironomoideaChironomidaeChironominaeChironomus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13711371Trifunctional purine biosynthetic protein adenosine-3PRO_0000074933Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ26255.
SMRiQ26255. Positions 494-792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 321207ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 1171738AIRSAdd
BLAST
Regioni1172 – 1371200GARTAdd
BLAST
Regioni1180 – 118235'-phosphoribosylglycinamide bindingBy similarity
Regioni1260 – 1263410-formyltetrahydrofolate bindingBy similarity
Regioni1311 – 1315510-formyltetrahydrofolate bindingBy similarity
Regioni1341 – 134445'-phosphoribosylglycinamide bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q26255 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGKKLLLIG SGGREHALAW KLQQSKNVTE IFAFPGSIGI SQLEKVQLVN
60 70 80 90 100
NNEMNLKDFK GIASWCKINH IDLVIVGPED PLAEGIADQL KAANIHCFGP
110 120 130 140 150
SKAGARIESD KSWSKDFMIR HHIPTAQYGS FIDALKAKDF IRNTPNALVV
160 170 180 190 200
KASGLAAGKG VIVAENIEEA CAAVDEILGD HKFGTAGDVV VVEEKLSGQE
210 220 230 240 250
VSVLGFVDSN SVRILPPAQD HKRLKDNDEG LNTGGMGAYC PCPLISQQEL
260 270 280 290 300
DIVKSQVLQR AVDGLRKENI LYNGILYAGI MLTHDGPKTL EFNCRFGDPE
310 320 330 340 350
TQIILPLLDE DLYDLMMASC TNHLCNVPEL KFKSNINAVG VVMASKGYPE
360 370 380 390 400
TSTKGCVISG IESVETMDNH IVFHSGTSKN NKDEWITNGG RVLINIALAD
410 420 430 440 450
NLKKAADLAT KACDVVKFDG SQYRRDIGKK AFQIHSLTYK ESGVNIEAGN
460 470 480 490 500
SLVGRIKSLS YGTHRSGVVG QIGSFGGLMR LNDIKYINSN GEESNYKDIV
510 520 530 540 550
LVQGTDGVGT KLKIAESMNV WDTIGIDLVA MCVNDVLCNG AEPIGFLDYI
560 570 580 590 600
ACGHLEVPTV ATIVKGIADG CRKANCALIG GETAEMPSMY GKGKYDLAGY
610 620 630 640 650
CVGITEYDEI LPKINDVHVG DVVIGLPSSG IHSNGFSLVN KIFQQTGFKL
660 670 680 690 700
TDIAEFSDSH KSYGMEFLTP TRLYVSETLP FLRNGYVKAL AHITGGGLLE
710 720 730 740 750
NIPRILPNHL SVQIDALTWK LPKVFSWLAA HGNVNANEML RTFNCGIGMI
760 770 780 790 800
IIMPRNDIEW ETIPEARMIG SITQRDHNGP QVIVKNFKEV LHKEVTHWKK
810 820 830 840 850
GDAETTSISY KDSGVDITAG NELVDNIKPH AKSTNRKGVI GGLGSFGGLF
860 870 880 890 900
RINECGTKFE DPMLVLATDG VGTKLKIAQQ LGKHDTVGID LVAMCNNDIL
910 920 930 940 950
CNGAEPLTFL DYFACGKLDV NVATNVVSGI AEGCRQSDST LLGGETAEMP
960 970 980 990 1000
GMYNPNVYDL AGFSLGVAEH EDILPKKNCL EVGDIIIGFP SNGVHSNGFS
1010 1020 1030 1040 1050
LIHKLFELTG YKWTDIAPFS AYGKTFGEEF LEPTKVYVKE ISPALKTGYV
1060 1070 1080 1090 1100
KALAHITGGG LWDNIPRVLP YNLTAELDAK KINISPVFAW LSLNGNIDKL
1110 1120 1130 1140 1150
ELLKTFNCGI GMIMIASKEH ELEILKSLYG SRASVIGKII PTKPHGHQVI
1160 1170 1180 1190 1200
VRHFATCFER VERLLSIPKK RVGVLISGSG SNLQALIDAT KSTNMGMCSE
1210 1220 1230 1240 1250
IVFVLSNKAG IFGLERAAKA NIPSTVISNK DYATREAFDV ALHNELIKHN
1260 1270 1280 1290 1300
VEIICLAGFM RILTPCFVNK WKGKLLNIHP SLLPKYKGIT AQKDALESGD
1310 1320 1330 1340 1350
NESGCTVHFV DENVDTGAII VQEIVPIFEN DTVESLTERI HVAEHIAFPK
1360 1370
ALRLVASGYV RLNDKCETEW A
Length:1,371
Mass (Da):149,103
Last modified:November 1, 1996 - v1
Checksum:i7BF4664DB1538946
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S43653 Genomic DNA. Translation: AAB23115.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S43653 Genomic DNA. Translation: AAB23115.1 .

3D structure databases

ProteinModelPortali Q26255.
SMRi Q26255. Positions 494-792.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00126 .
UPA00074 ; UER00129 .

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPi MF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Unusual organizational features of the Drosophila Gart locus are not conserved within Diptera."
    Clark D.V., Henikoff S.
    J. Mol. Evol. 35:51-59(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPUR2_CHITE
AccessioniPrimary (citable) accession number: Q26255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3