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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Chironomus tentans (Midge) (Camptochironomus tentans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291ManganeseBy similarity1
Metal bindingi293ManganeseBy similarity1
Binding sitei123510-formyltetrahydrofolateBy similarity1
Binding sitei127710-formyltetrahydrofolateBy similarity1
Active sitei1279Proton donorBy similarity1
Sitei1315Raises pKa of active site HisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 202ATPBy similarityAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125
UPA00074; UER00126
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiChironomus tentans (Midge) (Camptochironomus tentans)
Taxonomic identifieri7153 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraChironomoideaChironomidaeChironominaeChironomus

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749331 – 1371Trifunctional purine biosynthetic protein adenosine-3Add BLAST1371

Proteomic databases

PRIDEiQ26255

Structurei

3D structure databases

ProteinModelPortaliQ26255
SMRiQ26255
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 321ATP-graspAdd BLAST207

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni434 – 1171AIRSAdd BLAST738
Regioni1172 – 1371GARTAdd BLAST200
Regioni1180 – 11825'-phosphoribosylglycinamide bindingBy similarity3
Regioni1260 – 126310-formyltetrahydrofolate bindingBy similarity4
Regioni1311 – 131510-formyltetrahydrofolate bindingBy similarity5
Regioni1341 – 13445'-phosphoribosylglycinamide bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated

Family and domain databases

CDDicd08645 FMT_core_GART, 1 hit
cd02196 PurM, 2 hits
Gene3Di3.30.1330.10, 2 hits
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00741 AIRS, 2 hits
MF_00138 GARS, 1 hit
MF_01930 PurN, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR002376 Formyl_transf_N
IPR036477 Formyl_transf_N_sf
IPR004607 GART
IPR001555 GART_AS
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PF00551 Formyl_trans_N, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF53328 SSF53328, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 2 hits
TIGR00639 PurN, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit
PS00373 GART, 1 hit

Sequencei

Sequence statusi: Complete.

Q26255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGKKLLLIG SGGREHALAW KLQQSKNVTE IFAFPGSIGI SQLEKVQLVN
60 70 80 90 100
NNEMNLKDFK GIASWCKINH IDLVIVGPED PLAEGIADQL KAANIHCFGP
110 120 130 140 150
SKAGARIESD KSWSKDFMIR HHIPTAQYGS FIDALKAKDF IRNTPNALVV
160 170 180 190 200
KASGLAAGKG VIVAENIEEA CAAVDEILGD HKFGTAGDVV VVEEKLSGQE
210 220 230 240 250
VSVLGFVDSN SVRILPPAQD HKRLKDNDEG LNTGGMGAYC PCPLISQQEL
260 270 280 290 300
DIVKSQVLQR AVDGLRKENI LYNGILYAGI MLTHDGPKTL EFNCRFGDPE
310 320 330 340 350
TQIILPLLDE DLYDLMMASC TNHLCNVPEL KFKSNINAVG VVMASKGYPE
360 370 380 390 400
TSTKGCVISG IESVETMDNH IVFHSGTSKN NKDEWITNGG RVLINIALAD
410 420 430 440 450
NLKKAADLAT KACDVVKFDG SQYRRDIGKK AFQIHSLTYK ESGVNIEAGN
460 470 480 490 500
SLVGRIKSLS YGTHRSGVVG QIGSFGGLMR LNDIKYINSN GEESNYKDIV
510 520 530 540 550
LVQGTDGVGT KLKIAESMNV WDTIGIDLVA MCVNDVLCNG AEPIGFLDYI
560 570 580 590 600
ACGHLEVPTV ATIVKGIADG CRKANCALIG GETAEMPSMY GKGKYDLAGY
610 620 630 640 650
CVGITEYDEI LPKINDVHVG DVVIGLPSSG IHSNGFSLVN KIFQQTGFKL
660 670 680 690 700
TDIAEFSDSH KSYGMEFLTP TRLYVSETLP FLRNGYVKAL AHITGGGLLE
710 720 730 740 750
NIPRILPNHL SVQIDALTWK LPKVFSWLAA HGNVNANEML RTFNCGIGMI
760 770 780 790 800
IIMPRNDIEW ETIPEARMIG SITQRDHNGP QVIVKNFKEV LHKEVTHWKK
810 820 830 840 850
GDAETTSISY KDSGVDITAG NELVDNIKPH AKSTNRKGVI GGLGSFGGLF
860 870 880 890 900
RINECGTKFE DPMLVLATDG VGTKLKIAQQ LGKHDTVGID LVAMCNNDIL
910 920 930 940 950
CNGAEPLTFL DYFACGKLDV NVATNVVSGI AEGCRQSDST LLGGETAEMP
960 970 980 990 1000
GMYNPNVYDL AGFSLGVAEH EDILPKKNCL EVGDIIIGFP SNGVHSNGFS
1010 1020 1030 1040 1050
LIHKLFELTG YKWTDIAPFS AYGKTFGEEF LEPTKVYVKE ISPALKTGYV
1060 1070 1080 1090 1100
KALAHITGGG LWDNIPRVLP YNLTAELDAK KINISPVFAW LSLNGNIDKL
1110 1120 1130 1140 1150
ELLKTFNCGI GMIMIASKEH ELEILKSLYG SRASVIGKII PTKPHGHQVI
1160 1170 1180 1190 1200
VRHFATCFER VERLLSIPKK RVGVLISGSG SNLQALIDAT KSTNMGMCSE
1210 1220 1230 1240 1250
IVFVLSNKAG IFGLERAAKA NIPSTVISNK DYATREAFDV ALHNELIKHN
1260 1270 1280 1290 1300
VEIICLAGFM RILTPCFVNK WKGKLLNIHP SLLPKYKGIT AQKDALESGD
1310 1320 1330 1340 1350
NESGCTVHFV DENVDTGAII VQEIVPIFEN DTVESLTERI HVAEHIAFPK
1360 1370
ALRLVASGYV RLNDKCETEW A
Length:1,371
Mass (Da):149,103
Last modified:November 1, 1996 - v1
Checksum:i7BF4664DB1538946
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43653 Genomic DNA Translation: AAB23115.1

Similar proteinsi

Entry informationi

Entry nameiPUR2_CHITE
AccessioniPrimary (citable) accession number: Q26255
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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