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Protein

Nitrophorin-2

Gene
N/A
Organism
Rhodnius prolixus (Triatomid bug)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).By similarity
Specifically inhibits factor IXa-catalyzed activation of factor X in the presence of calcium and phospholipids irrespective of the presence or absence of factor VIIIa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi80 – 801Iron (heme proximal ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Vasoactive, Vasodilator

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrophorin-2
Short name:
NP2
Alternative name(s):
Prolixin-S
OrganismiRhodnius prolixus (Triatomid bug)
Taxonomic identifieri13249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraEuhemipteraHeteropteraPanheteropteraCimicomorphaReduviidaeTriatominaeRhodnius
Proteomesi
  • UP000015103 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 202179Nitrophorin-2PRO_0000021824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 144
Disulfide bondi62 ↔ 193

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the salivary glands.

Structurei

Secondary structure

1
202
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 404Combined sources
Beta strandi41 – 433Combined sources
Beta strandi45 – 517Combined sources
Beta strandi56 – 594Combined sources
Beta strandi61 – 7010Combined sources
Beta strandi73 – 8210Combined sources
Turni83 – 864Combined sources
Beta strandi87 – 9711Combined sources
Beta strandi99 – 11113Combined sources
Beta strandi117 – 1193Combined sources
Beta strandi125 – 13410Combined sources
Beta strandi136 – 14712Combined sources
Beta strandi150 – 16213Combined sources
Helixi169 – 1779Combined sources
Helixi182 – 1843Combined sources
Beta strandi185 – 1873Combined sources
Helixi188 – 1903Combined sources
Helixi197 – 2004Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUOX-ray2.00A24-202[»]
1PEEX-ray1.50A24-202[»]
1PM1X-ray1.10X24-202[»]
1T68X-ray1.45X24-202[»]
2A3FX-ray1.40X24-202[»]
2ACPX-ray1.40X24-202[»]
2AH7X-ray1.70X24-202[»]
2AL0X-ray1.60X24-202[»]
2ALLX-ray1.47X24-202[»]
2AMMX-ray1.90X24-202[»]
2ASNX-ray1.70X25-202[»]
2EU7X-ray1.20X24-202[»]
2GTFX-ray1.40X24-202[»]
2HYSX-ray1.20A24-202[»]
ProteinModelPortaliQ26241.
SMRiQ26241. Positions 24-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ26241.

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. Nitrophorin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR023613. Nitrophorin.
IPR002351. Nitrophorin_domain.
[Graphical view]
PfamiPF02087. Nitrophorin. 1 hit.
[Graphical view]
PRINTSiPR00788. NITROPHORIN.
SUPFAMiSSF50814. SSF50814. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q26241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELYTALLAV TILCLTSTMG VSGDCSTNIS PKQGLDKAKY FSGKWYVTHF
60 70 80 90 100
LDKDPQVTDQ YCSSFTPRES DGTVKEALYH YNANKKTSFY NIGEGKLESS
110 120 130 140 150
GLQYTAKYKT VDKKKAVLKE ADEKNSYTLT VLEADDSSAL VHICLREGSK
160 170 180 190 200
DLGDLYTVLT HQKDAEPSAK VKSAVTQAGL QLSQFVGTKD LGCQYDDQFT

SL
Length:202
Mass (Da):22,292
Last modified:November 1, 1996 - v1
Checksum:i312E96CB165D385C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61144 mRNA. Translation: AAB41587.1.
U70582 mRNA. Translation: AAB09588.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61144 mRNA. Translation: AAB41587.1.
U70582 mRNA. Translation: AAB09588.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUOX-ray2.00A24-202[»]
1PEEX-ray1.50A24-202[»]
1PM1X-ray1.10X24-202[»]
1T68X-ray1.45X24-202[»]
2A3FX-ray1.40X24-202[»]
2ACPX-ray1.40X24-202[»]
2AH7X-ray1.70X24-202[»]
2AL0X-ray1.60X24-202[»]
2ALLX-ray1.47X24-202[»]
2AMMX-ray1.90X24-202[»]
2ASNX-ray1.70X25-202[»]
2EU7X-ray1.20X24-202[»]
2GTFX-ray1.40X24-202[»]
2HYSX-ray1.20A24-202[»]
ProteinModelPortaliQ26241.
SMRiQ26241. Positions 24-202.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ26241.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR023613. Nitrophorin.
IPR002351. Nitrophorin_domain.
[Graphical view]
PfamiPF02087. Nitrophorin. 1 hit.
[Graphical view]
PRINTSiPR00788. NITROPHORIN.
SUPFAMiSSF50814. SSF50814. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification, characterization and cDNA cloning of a novel anticoagulant of the intrinsic pathway, (prolixin-S) from salivary glands of the blood sucking bug, Rhodnius prolixus."
    Sun J., Yamaguchi M., Yuda M., Miura K., Takeya H., Hirai M., Matsuoka H., Ando K., Watanabe T., Suzuki K., Chinzei Y.
    Thromb. Haemost. 75:573-577(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Salivary gland.
  2. Champagne D.E., Ribeiro J.M.C.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Salivary gland.
  3. "The insect salivary protein, prolixin-S, inhibits factor IXa generation and Xase complex formation in the blood coagulation pathway."
    Isawa H., Yuda M., Yoneda K., Chinzei Y.
    J. Biol. Chem. 275:6636-6641(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "The crystal structure of nitrophorin 2: a trifunctional antihemostatic protein from the saliva of Rhodnius prolixus."
    Andersen J.F., Montfort W.R.
    J. Biol. Chem. 275:30496-30503(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiNP2_RHOPR
AccessioniPrimary (citable) accession number: Q26241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 14, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.