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Q26240

- NOS_RHOPR

UniProt

Q26240 - NOS_RHOPR

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Protein

Nitric oxide synthase, salivary gland

Gene
N/A
Organism
Rhodnius prolixus (Triatomid bug)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. The production of NO in the salivary gland is used as a vasodilator for blood sucking.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi162 – 1621Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi639 – 67032FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi780 – 79112FADBy similarityAdd
BLAST
Nucleotide bindingi923 – 93311FADBy similarityAdd
BLAST
Nucleotide bindingi998 – 101619NADPBy similarityAdd
BLAST
Nucleotide bindingi1095 – 111016NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, salivary gland (EC:1.14.13.39)
Short name:
NOS
OrganismiRhodnius prolixus (Triatomid bug)
Taxonomic identifieri13249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraEuhemipteraHeteropteraPanheteropteraCimicomorphaReduviidaeTriatominaeRhodnius
ProteomesiUP000015103: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11741174Nitric oxide synthase, salivary glandPRO_0000170952Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ26240.
SMRiQ26240. Positions 50-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini505 – 693189Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini745 – 990246FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni475 – 49521Calmodulin-bindingSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 4511Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q26240-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVHAECVWWL GIRILFVPPV SLEMHSVNVN NMSIQQQQQH QPQQQLLKPI
60 70 80 90 100
RLANVSTQAQ SLDTLHYKCQ QEGPCMEQAC LASVIYAGVN LKPRVRPKEE
110 120 130 140 150
LLAHAKDFLD QYFASIRRLQ SPAHEARWAQ VEKEVAATGT YELTETELVY
160 170 180 190 200
GAKLAWRNAP RCIGRIQWAK LQVFDCRQVT TTSGMFEALC NHIKYSTNKG
210 220 230 240 250
NIRSAITIFP HRTDGKHDFR IWNKQLISYA GHKSKDGTVI GDPACVEFTE
260 270 280 290 300
ICIKLGWKGK GTMFDVLPLV LSANGEDPDY FDLPPELVFE VPLSHPKYKW
310 320 330 340 350
FSELGLKWFA LPAVSGMMFD CGGLQFTAAP FNGWYMNSEI GSRNLGDTNR
360 370 380 390 400
YNMLEKIAQK MELDTRTPVT LWKDLAMVEA NVAVLHSFQL HNVTIVDHHS
410 420 430 440 450
AAESFMKHLE NEQRLRGGCP ADWVWIVPPI SGSATPVFFQ EMANYFLYPG
460 470 480 490 500
YIYQEDAWKC HEWKEIDVKH GLKKEKRKFH FKQIARAVKF TSKLFGSALS
510 520 530 540 550
KRIKATILFA TETGKSEMYA RKLGDIFSHA FHSQVLSMED YDMSKIEHEA
560 570 580 590 600
LLLVVASTFG NGDPPENGQG FAQSLYTIKM DENGLPNGHT NNTLASSASF
610 620 630 640 650
IKANSQTDRQ ASLERCDSFR GSTGDADVFG PLSNVRFAVF ALGSSAYPNF
660 670 680 690 700
CAFGSYVDNL LGELGGERLV KLTTGDEMCG QAQACNKWAP EVFSVACDTF
710 720 730 740 750
CLDSDETFLE ATQMLHSEAV TASTVRFVES ATQDLCKALS HLHNKKVWKC
760 770 780 790 800
PLLGKRNLHG KGSTRATLLL EIERNENISY QPGDHVGVLA CNRKELVEGI
810 820 830 840 850
ISHLESAIDP DKSVQLQILK ENTTPDGIVR NWIPHDRLPT CSLRTMLTRF
860 870 880 890 900
LDITTPPSPN LLQFFASCAT NSEDQEKLTE LATDSAAYED WRYWKYPNLL
910 920 930 940 950
EVLEEFPSVR VLPALLIAQL TPLQPRFYSI SSAPSLYANQ IHLTVAVVQY
960 970 980 990 1000
CTQDGKGPIH YGVASNYLYD VTIGDSIYLF TRSAPNFHLP KSDTAPIIMV
1010 1020 1030 1040 1050
GPGTGIAPFR GFWQHRLAQR SLNGPGKFGK MSLFFGCRLR NLDLYQEEKE
1060 1070 1080 1090 1100
SMLKEGILSK VFLALSREPS IPKTYVQDLL RVECKSVYIQ IVQEGGHFYV
1110 1120 1130 1140 1150
CGDCTMAEHV FRTLRQIIQD QGNMTDHQVD NFMLAMRDEN RYHEDIFGIT
1160 1170
LRTAEVHNRS RESARIRMAS QSQP
Length:1,174
Mass (Da):132,393
Last modified:November 1, 1996 - v1
Checksum:iC32F664EE51409CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59389 mRNA. Translation: AAB03810.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59389 mRNA. Translation: AAB03810.1 .

3D structure databases

ProteinModelPortali Q26240.
SMRi Q26240. Positions 50-464.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus."
    Yuda M., Hirai M., Miura K., Matsumura H., Ando K., Chinzei Y.
    Eur. J. Biochem. 242:807-812(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Salivary gland.

Entry informationi

Entry nameiNOS_RHOPR
AccessioniPrimary (citable) accession number: Q26240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3