Reviewed, UniProtKB/Swiss-Prot Q26240 (NOS_RHOPR)

Names and origin

Protein names	Recommended name:     Nitric oxide synthase, salivary gland       Short name=NOS     EC=1.14.13.39
Organism	Rhodnius prolixus (Triatomid bug)
Taxonomic identifier	13249 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Paraneoptera › Hemiptera › Euhemiptera › Heteroptera › Panheteroptera › Cimicomorpha › Reduviidae › Triatominae › Rhodnius

Protein attributes

Sequence length	1174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. The production of NO in the salivary gland is used as a vasodilator for blood sucking.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity.
Enzyme regulation	Stimulated by calcium/calmodulin.
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro calmodulin binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1174	1174	Nitric oxide synthase, salivary gland		PRO_0000170952
Regions
	Domain	505 – 693	189	Flavodoxin-like
	Domain	745 – 990	246	FAD-binding FR-type
	Nucleotide binding	639 – 670	32	FMN By similarity
	Nucleotide binding	780 – 791	12	FAD By similarity
	Nucleotide binding	923 – 933	11	FAD By similarity
	Nucleotide binding	998 – 1016	19	NADP By similarity
	Nucleotide binding	1095 – 1110	16	NADP By similarity
	Region	475 – 495	21	Calmodulin-binding Potential
	Compositional bias	35 – 45	11	Poly-Gln
Sites
	Metal binding	162	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q26240-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C32F664EE51409CF	FASTA	1,174	132,393
	10 20 30 40 50 60 MVHAECVWWL GIRILFVPPV SLEMHSVNVN NMSIQQQQQH QPQQQLLKPI RLANVSTQAQ 70 80 90 100 110 120 SLDTLHYKCQ QEGPCMEQAC LASVIYAGVN LKPRVRPKEE LLAHAKDFLD QYFASIRRLQ 130 140 150 160 170 180 SPAHEARWAQ VEKEVAATGT YELTETELVY GAKLAWRNAP RCIGRIQWAK LQVFDCRQVT 190 200 210 220 230 240 TTSGMFEALC NHIKYSTNKG NIRSAITIFP HRTDGKHDFR IWNKQLISYA GHKSKDGTVI 250 260 270 280 290 300 GDPACVEFTE ICIKLGWKGK GTMFDVLPLV LSANGEDPDY FDLPPELVFE VPLSHPKYKW 310 320 330 340 350 360 FSELGLKWFA LPAVSGMMFD CGGLQFTAAP FNGWYMNSEI GSRNLGDTNR YNMLEKIAQK 370 380 390 400 410 420 MELDTRTPVT LWKDLAMVEA NVAVLHSFQL HNVTIVDHHS AAESFMKHLE NEQRLRGGCP 430 440 450 460 470 480 ADWVWIVPPI SGSATPVFFQ EMANYFLYPG YIYQEDAWKC HEWKEIDVKH GLKKEKRKFH 490 500 510 520 530 540 FKQIARAVKF TSKLFGSALS KRIKATILFA TETGKSEMYA RKLGDIFSHA FHSQVLSMED 550 560 570 580 590 600 YDMSKIEHEA LLLVVASTFG NGDPPENGQG FAQSLYTIKM DENGLPNGHT NNTLASSASF 610 620 630 640 650 660 IKANSQTDRQ ASLERCDSFR GSTGDADVFG PLSNVRFAVF ALGSSAYPNF CAFGSYVDNL 670 680 690 700 710 720 LGELGGERLV KLTTGDEMCG QAQACNKWAP EVFSVACDTF CLDSDETFLE ATQMLHSEAV 730 740 750 760 770 780 TASTVRFVES ATQDLCKALS HLHNKKVWKC PLLGKRNLHG KGSTRATLLL EIERNENISY 790 800 810 820 830 840 QPGDHVGVLA CNRKELVEGI ISHLESAIDP DKSVQLQILK ENTTPDGIVR NWIPHDRLPT 850 860 870 880 890 900 CSLRTMLTRF LDITTPPSPN LLQFFASCAT NSEDQEKLTE LATDSAAYED WRYWKYPNLL 910 920 930 940 950 960 EVLEEFPSVR VLPALLIAQL TPLQPRFYSI SSAPSLYANQ IHLTVAVVQY CTQDGKGPIH 970 980 990 1000 1010 1020 YGVASNYLYD VTIGDSIYLF TRSAPNFHLP KSDTAPIIMV GPGTGIAPFR GFWQHRLAQR 1030 1040 1050 1060 1070 1080 SLNGPGKFGK MSLFFGCRLR NLDLYQEEKE SMLKEGILSK VFLALSREPS IPKTYVQDLL 1090 1100 1110 1120 1130 1140 RVECKSVYIQ IVQEGGHFYV CGDCTMAEHV FRTLRQIIQD QGNMTDHQVD NFMLAMRDEN 1150 1160 1170 RYHEDIFGIT LRTAEVHNRS RESARIRMAS QSQP

References

[1]

"cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus." Yuda M., Hirai M., Miura K., Matsumura H., Ando K., Chinzei Y. Eur. J. Biochem. 242:807-812(1996) [PubMed: 9022713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Salivary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U59389 mRNA. Translation: AAB03810.1.
3D structure databases
HSSP	HSSP built from PDB template 1DD7 based on UniProtKB P29477.
ModBase	Search...
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR004030. NO_synthase_oxygenase_reg. IPR012144. NOS. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Gene3D	G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000333. NOS. 1 hit.
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS_RHOPR

Accession

Primary (citable) accession number: Q26240

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)