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Q26240 (NOS_RHOPR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitric oxide synthase, salivary gland

Short name=NOS
EC=1.14.13.39
OrganismRhodnius prolixus (Triatomid bug) [Complete proteome]
Taxonomic identifier13249 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraEuhemipteraHeteropteraPanheteropteraCimicomorphaReduviidaeTriatominaeRhodnius

Protein attributes

Sequence length1174 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. The production of NO in the salivary gland is used as a vasodilator for blood sucking.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin.

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11741174Nitric oxide synthase, salivary gland
PRO_0000170952

Regions

Domain505 – 693189Flavodoxin-like
Domain745 – 990246FAD-binding FR-type
Nucleotide binding639 – 67032FMN By similarity
Nucleotide binding780 – 79112FAD By similarity
Nucleotide binding923 – 93311FAD By similarity
Nucleotide binding998 – 101619NADP By similarity
Nucleotide binding1095 – 111016NADP By similarity
Region475 – 49521Calmodulin-binding Potential
Compositional bias35 – 4511Poly-Gln

Sites

Metal binding1621Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q26240 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C32F664EE51409CF

FASTA1,174132,393
        10         20         30         40         50         60 
MVHAECVWWL GIRILFVPPV SLEMHSVNVN NMSIQQQQQH QPQQQLLKPI RLANVSTQAQ 

        70         80         90        100        110        120 
SLDTLHYKCQ QEGPCMEQAC LASVIYAGVN LKPRVRPKEE LLAHAKDFLD QYFASIRRLQ 

       130        140        150        160        170        180 
SPAHEARWAQ VEKEVAATGT YELTETELVY GAKLAWRNAP RCIGRIQWAK LQVFDCRQVT 

       190        200        210        220        230        240 
TTSGMFEALC NHIKYSTNKG NIRSAITIFP HRTDGKHDFR IWNKQLISYA GHKSKDGTVI 

       250        260        270        280        290        300 
GDPACVEFTE ICIKLGWKGK GTMFDVLPLV LSANGEDPDY FDLPPELVFE VPLSHPKYKW 

       310        320        330        340        350        360 
FSELGLKWFA LPAVSGMMFD CGGLQFTAAP FNGWYMNSEI GSRNLGDTNR YNMLEKIAQK 

       370        380        390        400        410        420 
MELDTRTPVT LWKDLAMVEA NVAVLHSFQL HNVTIVDHHS AAESFMKHLE NEQRLRGGCP 

       430        440        450        460        470        480 
ADWVWIVPPI SGSATPVFFQ EMANYFLYPG YIYQEDAWKC HEWKEIDVKH GLKKEKRKFH 

       490        500        510        520        530        540 
FKQIARAVKF TSKLFGSALS KRIKATILFA TETGKSEMYA RKLGDIFSHA FHSQVLSMED 

       550        560        570        580        590        600 
YDMSKIEHEA LLLVVASTFG NGDPPENGQG FAQSLYTIKM DENGLPNGHT NNTLASSASF 

       610        620        630        640        650        660 
IKANSQTDRQ ASLERCDSFR GSTGDADVFG PLSNVRFAVF ALGSSAYPNF CAFGSYVDNL 

       670        680        690        700        710        720 
LGELGGERLV KLTTGDEMCG QAQACNKWAP EVFSVACDTF CLDSDETFLE ATQMLHSEAV 

       730        740        750        760        770        780 
TASTVRFVES ATQDLCKALS HLHNKKVWKC PLLGKRNLHG KGSTRATLLL EIERNENISY 

       790        800        810        820        830        840 
QPGDHVGVLA CNRKELVEGI ISHLESAIDP DKSVQLQILK ENTTPDGIVR NWIPHDRLPT 

       850        860        870        880        890        900 
CSLRTMLTRF LDITTPPSPN LLQFFASCAT NSEDQEKLTE LATDSAAYED WRYWKYPNLL 

       910        920        930        940        950        960 
EVLEEFPSVR VLPALLIAQL TPLQPRFYSI SSAPSLYANQ IHLTVAVVQY CTQDGKGPIH 

       970        980        990       1000       1010       1020 
YGVASNYLYD VTIGDSIYLF TRSAPNFHLP KSDTAPIIMV GPGTGIAPFR GFWQHRLAQR 

      1030       1040       1050       1060       1070       1080 
SLNGPGKFGK MSLFFGCRLR NLDLYQEEKE SMLKEGILSK VFLALSREPS IPKTYVQDLL 

      1090       1100       1110       1120       1130       1140 
RVECKSVYIQ IVQEGGHFYV CGDCTMAEHV FRTLRQIIQD QGNMTDHQVD NFMLAMRDEN 

      1150       1160       1170 
RYHEDIFGIT LRTAEVHNRS RESARIRMAS QSQP 

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References

[1]"cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus."
Yuda M., Hirai M., Miura K., Matsumura H., Ando K., Chinzei Y.
Eur. J. Biochem. 242:807-812(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Salivary gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59389 mRNA. Translation: AAB03810.1.

3D structure databases

ProteinModelPortalQ26240.
SMRQ26240. Positions 50-464.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS_RHOPR
AccessionPrimary (citable) accession number: Q26240
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families