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Q26240

- NOS_RHOPR

UniProt

Q26240 - NOS_RHOPR

Protein

Nitric oxide synthase, salivary gland

Gene
N/A
Organism
Rhodnius prolixus (Triatomid bug)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. The production of NO in the salivary gland is used as a vasodilator for blood sucking.

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity

    Enzyme regulationi

    Stimulated by calcium/calmodulin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi162 – 1621Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi639 – 67032FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi780 – 79112FADBy similarityAdd
    BLAST
    Nucleotide bindingi923 – 93311FADBy similarityAdd
    BLAST
    Nucleotide bindingi998 – 101619NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1095 – 111016NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, salivary gland (EC:1.14.13.39)
    Short name:
    NOS
    OrganismiRhodnius prolixus (Triatomid bug)
    Taxonomic identifieri13249 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraEuhemipteraHeteropteraPanheteropteraCimicomorphaReduviidaeTriatominaeRhodnius
    ProteomesiUP000015103: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11741174Nitric oxide synthase, salivary glandPRO_0000170952Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ26240.
    SMRiQ26240. Positions 50-464.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini505 – 693189Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini745 – 990246FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni475 – 49521Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi35 – 4511Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q26240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVHAECVWWL GIRILFVPPV SLEMHSVNVN NMSIQQQQQH QPQQQLLKPI     50
    RLANVSTQAQ SLDTLHYKCQ QEGPCMEQAC LASVIYAGVN LKPRVRPKEE 100
    LLAHAKDFLD QYFASIRRLQ SPAHEARWAQ VEKEVAATGT YELTETELVY 150
    GAKLAWRNAP RCIGRIQWAK LQVFDCRQVT TTSGMFEALC NHIKYSTNKG 200
    NIRSAITIFP HRTDGKHDFR IWNKQLISYA GHKSKDGTVI GDPACVEFTE 250
    ICIKLGWKGK GTMFDVLPLV LSANGEDPDY FDLPPELVFE VPLSHPKYKW 300
    FSELGLKWFA LPAVSGMMFD CGGLQFTAAP FNGWYMNSEI GSRNLGDTNR 350
    YNMLEKIAQK MELDTRTPVT LWKDLAMVEA NVAVLHSFQL HNVTIVDHHS 400
    AAESFMKHLE NEQRLRGGCP ADWVWIVPPI SGSATPVFFQ EMANYFLYPG 450
    YIYQEDAWKC HEWKEIDVKH GLKKEKRKFH FKQIARAVKF TSKLFGSALS 500
    KRIKATILFA TETGKSEMYA RKLGDIFSHA FHSQVLSMED YDMSKIEHEA 550
    LLLVVASTFG NGDPPENGQG FAQSLYTIKM DENGLPNGHT NNTLASSASF 600
    IKANSQTDRQ ASLERCDSFR GSTGDADVFG PLSNVRFAVF ALGSSAYPNF 650
    CAFGSYVDNL LGELGGERLV KLTTGDEMCG QAQACNKWAP EVFSVACDTF 700
    CLDSDETFLE ATQMLHSEAV TASTVRFVES ATQDLCKALS HLHNKKVWKC 750
    PLLGKRNLHG KGSTRATLLL EIERNENISY QPGDHVGVLA CNRKELVEGI 800
    ISHLESAIDP DKSVQLQILK ENTTPDGIVR NWIPHDRLPT CSLRTMLTRF 850
    LDITTPPSPN LLQFFASCAT NSEDQEKLTE LATDSAAYED WRYWKYPNLL 900
    EVLEEFPSVR VLPALLIAQL TPLQPRFYSI SSAPSLYANQ IHLTVAVVQY 950
    CTQDGKGPIH YGVASNYLYD VTIGDSIYLF TRSAPNFHLP KSDTAPIIMV 1000
    GPGTGIAPFR GFWQHRLAQR SLNGPGKFGK MSLFFGCRLR NLDLYQEEKE 1050
    SMLKEGILSK VFLALSREPS IPKTYVQDLL RVECKSVYIQ IVQEGGHFYV 1100
    CGDCTMAEHV FRTLRQIIQD QGNMTDHQVD NFMLAMRDEN RYHEDIFGIT 1150
    LRTAEVHNRS RESARIRMAS QSQP 1174
    Length:1,174
    Mass (Da):132,393
    Last modified:November 1, 1996 - v1
    Checksum:iC32F664EE51409CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59389 mRNA. Translation: AAB03810.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59389 mRNA. Translation: AAB03810.1 .

    3D structure databases

    ProteinModelPortali Q26240.
    SMRi Q26240. Positions 50-464.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus."
      Yuda M., Hirai M., Miura K., Matsumura H., Ando K., Chinzei Y.
      Eur. J. Biochem. 242:807-812(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiNOS_RHOPR
    AccessioniPrimary (citable) accession number: Q26240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3