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Q26240

- NOS_RHOPR

UniProt

Q26240 - NOS_RHOPR

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Protein
Nitric oxide synthase, salivary gland
Gene
N/A
Organism
Rhodnius prolixus (Triatomid bug)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. The production of NO in the salivary gland is used as a vasodilator for blood sucking.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.

Enzyme regulationi

Stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi162 – 1621Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi639 – 67032FMN By similarity
Add
BLAST
Nucleotide bindingi780 – 79112FAD By similarity
Add
BLAST
Nucleotide bindingi923 – 93311FAD By similarity
Add
BLAST
Nucleotide bindingi998 – 101619NADP By similarity
Add
BLAST
Nucleotide bindingi1095 – 111016NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADP binding Source: InterPro
  3. flavin adenine dinucleotide binding Source: InterPro
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, salivary gland (EC:1.14.13.39)
Short name:
NOS
OrganismiRhodnius prolixus (Triatomid bug)
Taxonomic identifieri13249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraEuhemipteraHeteropteraPanheteropteraCimicomorphaReduviidaeTriatominaeRhodnius
ProteomesiUP000015103: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11741174Nitric oxide synthase, salivary gland
PRO_0000170952Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ26240.
SMRiQ26240. Positions 50-464.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini505 – 693189Flavodoxin-like
Add
BLAST
Domaini745 – 990246FAD-binding FR-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni475 – 49521Calmodulin-binding Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 4511Poly-Gln
Add
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q26240-1 [UniParc]FASTAAdd to Basket

« Hide

MVHAECVWWL GIRILFVPPV SLEMHSVNVN NMSIQQQQQH QPQQQLLKPI     50
RLANVSTQAQ SLDTLHYKCQ QEGPCMEQAC LASVIYAGVN LKPRVRPKEE 100
LLAHAKDFLD QYFASIRRLQ SPAHEARWAQ VEKEVAATGT YELTETELVY 150
GAKLAWRNAP RCIGRIQWAK LQVFDCRQVT TTSGMFEALC NHIKYSTNKG 200
NIRSAITIFP HRTDGKHDFR IWNKQLISYA GHKSKDGTVI GDPACVEFTE 250
ICIKLGWKGK GTMFDVLPLV LSANGEDPDY FDLPPELVFE VPLSHPKYKW 300
FSELGLKWFA LPAVSGMMFD CGGLQFTAAP FNGWYMNSEI GSRNLGDTNR 350
YNMLEKIAQK MELDTRTPVT LWKDLAMVEA NVAVLHSFQL HNVTIVDHHS 400
AAESFMKHLE NEQRLRGGCP ADWVWIVPPI SGSATPVFFQ EMANYFLYPG 450
YIYQEDAWKC HEWKEIDVKH GLKKEKRKFH FKQIARAVKF TSKLFGSALS 500
KRIKATILFA TETGKSEMYA RKLGDIFSHA FHSQVLSMED YDMSKIEHEA 550
LLLVVASTFG NGDPPENGQG FAQSLYTIKM DENGLPNGHT NNTLASSASF 600
IKANSQTDRQ ASLERCDSFR GSTGDADVFG PLSNVRFAVF ALGSSAYPNF 650
CAFGSYVDNL LGELGGERLV KLTTGDEMCG QAQACNKWAP EVFSVACDTF 700
CLDSDETFLE ATQMLHSEAV TASTVRFVES ATQDLCKALS HLHNKKVWKC 750
PLLGKRNLHG KGSTRATLLL EIERNENISY QPGDHVGVLA CNRKELVEGI 800
ISHLESAIDP DKSVQLQILK ENTTPDGIVR NWIPHDRLPT CSLRTMLTRF 850
LDITTPPSPN LLQFFASCAT NSEDQEKLTE LATDSAAYED WRYWKYPNLL 900
EVLEEFPSVR VLPALLIAQL TPLQPRFYSI SSAPSLYANQ IHLTVAVVQY 950
CTQDGKGPIH YGVASNYLYD VTIGDSIYLF TRSAPNFHLP KSDTAPIIMV 1000
GPGTGIAPFR GFWQHRLAQR SLNGPGKFGK MSLFFGCRLR NLDLYQEEKE 1050
SMLKEGILSK VFLALSREPS IPKTYVQDLL RVECKSVYIQ IVQEGGHFYV 1100
CGDCTMAEHV FRTLRQIIQD QGNMTDHQVD NFMLAMRDEN RYHEDIFGIT 1150
LRTAEVHNRS RESARIRMAS QSQP 1174
Length:1,174
Mass (Da):132,393
Last modified:November 1, 1996 - v1
Checksum:iC32F664EE51409CF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59389 mRNA. Translation: AAB03810.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59389 mRNA. Translation: AAB03810.1 .

3D structure databases

ProteinModelPortali Q26240.
SMRi Q26240. Positions 50-464.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus."
    Yuda M., Hirai M., Miura K., Matsumura H., Ando K., Chinzei Y.
    Eur. J. Biochem. 242:807-812(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Salivary gland.

Entry informationi

Entry nameiNOS_RHOPR
AccessioniPrimary (citable) accession number: Q26240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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