Q26061 (FRI_PACLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ferritin EC=1.16.3.1
Organism	Pacifastacus leniusculus (Signal crayfish)
Taxonomic identifier	6720 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Astacidea › Astacoidea › Astacidae › Pacifastacus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation By similarity.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Subunit structure	Oligomer of 12 or 24 subunits. The functional molecule is roughly spherical and contains a central cavity into which the polymeric mineral iron core is deposited By similarity.
Subcellular location	Cytoplasm Potential.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	There are 2 types of crayfish ferritin subunit: a 19 kDa chain and a 20 kDa chain.
Sequence similarities	Belongs to the ferritin family. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Ferritin		PRO_0000201071
Regions
	Domain	6 – 155	150	Ferritin-like diiron
Sites
	Metal binding	23	1	Iron 1 By similarity
	Metal binding	58	1	Iron 1 By similarity
	Metal binding	58	1	Iron 2 By similarity
	Metal binding	61	1	Iron 1 By similarity
	Metal binding	103	1	Iron 2 By similarity
	Metal binding	137	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q26061 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C7318FC910178C55	FASTA	181	20,671
	10 20 30 40 50 60 MASQIRHNYH EDCEPINKQI NLEFYASYVY MSMGHYFDRD DISLPGASKF FKDSSDEERE 70 80 90 100 110 120 HGQKLMKYQN KRGARIVLQA IAAPSLQEWG NLHDALQAAL DLENEVNQSL LDLDATASKI 130 140 150 160 170 180 NDPHLTNMLE GEFLEEQVES IEKIGNLITR LKRAGTSGLG EFLFDKELKQ RFLPSLTSHP N

References

[1]

"Purification and cDNA cloning of ferritin from the hepatopancreas of the freshwater crayfish Pacifastacus leniusculus." Huang T.-S., Law J.H., Soederhaell K. Eur. J. Biochem. 236:450-456(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 76-88. Tissue: Hepatopancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90566 mRNA. Translation: CAA62186.1.
PIR	S62651.
3D structure databases
ProteinModelPortal	Q26061.
SMR	Q26061. Positions 2-168.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1260.10. 1 hit.
InterPro	IPR001519. Ferritin. IPR009040. Ferritin-like_diiron. IPR009078. Ferritin-like_SF. IPR012347. Ferritin-rel. IPR008331. Ferritin_DPS_dom. [Graphical view]
PANTHER	PTHR11431. PTHR11431. 1 hit.
Pfam	PF00210. Ferritin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00540. FERRITIN_1. False negative. PS00204. FERRITIN_2. False negative. PS50905. FERRITIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRI_PACLE

Accession

Primary (citable) accession number: Q26061

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)