Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Akirin-2

Gene

Akirin2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6 (By similarity). Forms a complex with YWHAB that acts to repress transcription of DUSP1.By similarity1 Publication

GO - Biological processi

  • embryo development Source: Ensembl
  • innate immune response Source: UniProtKB-KW
  • negative regulation of gene expression Source: RGD
  • negative regulation of transcription, DNA-templated Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of endopeptidase activity Source: RGD
  • positive regulation of interleukin-6 production Source: Ensembl
  • positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Immunity, Innate immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Akirin-2
Alternative name(s):
Fourteen-three-three beta interactant 1Imported
Fourteen-three-three beta interacting-protein 1
Gene namesi
Name:Akirin2By similarity
Synonyms:FBI1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1307791. Akirin2.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311S → A: Abolishes binding to YWHAB. Reduces transcriptional repressor activity; when associated with A-111 and A-119. 1 Publication
Mutagenesisi103 – 1031T → A: Abolishes binding to YWHAB. Loss of transcriptional repressor activity; when associated with A-111; A-119 and A-131. 1 Publication
Mutagenesisi111 – 1111S → A: Abolishes binding to YWHAB. Loss of transcriptional repressor activity; when associated with A-103; A-119 and A-131. Reduces transcriptional repressor activity; when associated with A-31 and A-119. 1 Publication
Mutagenesisi119 – 1191S → A: Abolishes binding to YWHAB. Loss of transcriptional repressor activity; when associated with A-103; A-111 and A-131. Reduces transcriptional repressor activity; when associated with A-31 and A-111. 1 Publication
Mutagenesisi131 – 1311S → A: Abolishes binding to YWHAB. Loss of transcriptional repressor activity; when associated with A-103; A-111 and A-119. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Akirin-2PRO_0000355122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphoserineBy similarity
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei55 – 551PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ25C79.
PRIDEiQ25C79.

Expressioni

Tissue specificityi

Highly expressed in testis, cerebrum and cerebellum, and barely detectable in liver, heart, spleen and muscle. Also highly expressed in various tumor cells from hepatoma, glioblastoma and pheochromocytoma.1 Publication

Gene expression databases

GenevisibleiQ25C79. RN.

Interactioni

Subunit structurei

Interacts with YWHAB.1 Publication

Protein-protein interaction databases

IntActiQ25C79. 1 interaction.
MINTiMINT-7997422.
STRINGi10116.ENSRNOP00000010962.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 276Nuclear localization signal

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 5427Ala-richSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the akirin family.Curated

Phylogenomic databases

eggNOGiKOG4330. Eukaryota.
ENOG4111J00. LUCA.
GeneTreeiENSGT00390000007905.
HOGENOMiHOG000231406.
HOVERGENiHBG055078.
InParanoidiQ25C79.
OMAiRAQMGTI.
OrthoDBiEOG72ZCGW.
PhylomeDBiQ25C79.
TreeFamiTF317123.

Family and domain databases

InterProiIPR024132. Akirin.
[Graphical view]
PANTHERiPTHR13293. PTHR13293. 1 hit.

Sequencei

Sequence statusi: Complete.

Q25C79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACGATLKRT LDFDPLLSPA SPKRRRCAPL SAPASAAASP AAATAAAAAS
60 70 80 90 100
AAAASPQKYL RMEPSPFGDV SSRLTTEQIL YNIKQEYKRM QKRRHLEASF
110 120 130 140 150
QQTDPGCSSD SQPHAFLISG PASPGTSSAT SSPLKKEQPL FTLRQVGMIC
160 170 180 190 200
ERLLKEREEK VREEYEEILN TKLAEQYDAF VKFTHDQIMR RYGEQPASYV

S
Length:201
Mass (Da):22,129
Last modified:April 18, 2006 - v1
Checksum:i2DEC2AA36F95C1BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB234867 mRNA. Translation: BAE81786.1.
CH473962 Genomic DNA. Translation: EDL98593.1.
RefSeqiNP_001035003.1. NM_001039914.2.
UniGeneiRn.3858.

Genome annotation databases

EnsembliENSRNOT00000010962; ENSRNOP00000010962; ENSRNOG00000008288.
GeneIDi297968.
KEGGirno:297968.
UCSCiRGD:1307791. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB234867 mRNA. Translation: BAE81786.1.
CH473962 Genomic DNA. Translation: EDL98593.1.
RefSeqiNP_001035003.1. NM_001039914.2.
UniGeneiRn.3858.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ25C79. 1 interaction.
MINTiMINT-7997422.
STRINGi10116.ENSRNOP00000010962.

Proteomic databases

PaxDbiQ25C79.
PRIDEiQ25C79.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010962; ENSRNOP00000010962; ENSRNOG00000008288.
GeneIDi297968.
KEGGirno:297968.
UCSCiRGD:1307791. rat.

Organism-specific databases

CTDi55122.
RGDi1307791. Akirin2.

Phylogenomic databases

eggNOGiKOG4330. Eukaryota.
ENOG4111J00. LUCA.
GeneTreeiENSGT00390000007905.
HOGENOMiHOG000231406.
HOVERGENiHBG055078.
InParanoidiQ25C79.
OMAiRAQMGTI.
OrthoDBiEOG72ZCGW.
PhylomeDBiQ25C79.
TreeFamiTF317123.

Miscellaneous databases

NextBioi642901.
PROiQ25C79.

Gene expression databases

GenevisibleiQ25C79. RN.

Family and domain databases

InterProiIPR024132. Akirin.
[Graphical view]
PANTHERiPTHR13293. PTHR13293. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel binding factor of 14-3-3beta functions as a transcriptional repressor and promotes anchorage-independent growth, tumorigenicity, and metastasis."
    Komiya Y., Kurabe N., Katagiri K., Ogawa M., Sugiyama A., Kawasaki Y., Tashiro F.
    J. Biol. Chem. 283:18753-18764(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH YWHAB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF SER-31; THR-103; SER-111; SER-119 AND SER-131.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAKIR2_RAT
AccessioniPrimary (citable) accession number: Q25C79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: April 18, 2006
Last modified: November 11, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.