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Protein

Beta-glucosidase 1A

Gene

BGL1A

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Kineticsi

  1. KM=6.80 mM for cellobiose2 Publications
  2. KM=0.229 mM for p-nitrophenyl-beta-D-glucoside (pNP-Glu)2 Publications
  3. KM=10.2 mM for p-nitrophenyl-beta-D-galactoside (pNP-Gal)2 Publications
  4. KM=0.752 mM for p-nitrophenyl-beta-D-xyloside (pNP-Xyl)2 Publications

    pH dependencei

    Optimum pH is 6.0-6.5.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei20Substrate1 Publication1
    Binding sitei123Substrate1 Publication1
    Binding sitei169Substrate1 Publication1
    Active sitei170Proton donor1 Publication1
    Binding sitei301Substrate1 Publication1
    Active sitei365Nucleophile1 Publication1
    Binding sitei415Substrate1 Publication1

    GO - Molecular functioni

    • beta-glucosidase activity Source: UniProtKB
    • cellobiose glucosidase activity Source: UniProtKB
    • scopolin beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    • cellulose catabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 1380.
    SABIO-RKQ25BW5.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1A_PHACH.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 1A1 PublicationImported (EC:3.2.1.21)
    Alternative name(s):
    Cellobiase 1A1 Publication
    Gene namesi
    Name:BGL1AImported
    OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
    Taxonomic identifieri5306 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi173V → C: 2-fold decrease in affinity for cellobiose. 1 Publication1
    Mutagenesisi177M → L: Small decrease in affinity for cellobiose. 1 Publication1
    Mutagenesisi229D → N: 17-fold decrease in affinity for cellobiose and displays more acidic optimum pH than wild-type. No effect on optimum pH; when associated with A-253. 1 Publication1
    Mutagenesisi231H → D: 3-fold decrease in affinity for cellobiose. 1 Publication1
    Mutagenesisi253K → A: 7-fold decrease in affinity for cellobiose. No effect on optimum pH; when associated with N-229. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003907871 – 462Beta-glucosidase 1AAdd BLAST462

    Proteomic databases

    PRIDEiQ25BW5.

    Expressioni

    Inductioni

    Expressed constitutively in cellobiose and glucose cultures.1 Publication

    Structurei

    Secondary structure

    1462
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi11 – 15Combined sources5
    Helixi18 – 21Combined sources4
    Helixi27 – 29Combined sources3
    Helixi34 – 38Combined sources5
    Beta strandi51 – 53Combined sources3
    Turni57 – 60Combined sources4
    Helixi61 – 70Combined sources10
    Beta strandi74 – 79Combined sources6
    Helixi82 – 85Combined sources4
    Helixi97 – 113Combined sources17
    Beta strandi116 – 124Combined sources9
    Helixi128 – 134Combined sources7
    Helixi136 – 138Combined sources3
    Helixi140 – 158Combined sources19
    Turni159 – 161Combined sources3
    Beta strandi164 – 169Combined sources6
    Helixi171 – 179Combined sources9
    Helixi193 – 215Combined sources23
    Helixi217 – 220Combined sources4
    Beta strandi223 – 229Combined sources7
    Beta strandi232 – 239Combined sources8
    Helixi240 – 253Combined sources14
    Helixi255 – 263Combined sources9
    Helixi268 – 274Combined sources7
    Helixi275 – 277Combined sources3
    Helixi283 – 289Combined sources7
    Beta strandi294 – 299Combined sources6
    Beta strandi303 – 308Combined sources6
    Helixi313 – 315Combined sources3
    Beta strandi317 – 323Combined sources7
    Beta strandi329 – 331Combined sources3
    Beta strandi333 – 336Combined sources4
    Helixi343 – 357Combined sources15
    Beta strandi361 – 366Combined sources6
    Helixi373 – 375Combined sources3
    Helixi378 – 381Combined sources4
    Helixi385 – 403Combined sources19
    Beta strandi409 – 415Combined sources7
    Helixi423 – 425Combined sources3
    Beta strandi433 – 436Combined sources4
    Turni438 – 440Combined sources3
    Beta strandi443 – 445Combined sources3
    Helixi447 – 459Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2E3ZX-ray1.50A/B1-462[»]
    2E40X-ray1.90A/B1-462[»]
    ProteinModelPortaliQ25BW5.
    SMRiQ25BW5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ25BW5.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni422 – 423Substrate binding2

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Sequence analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR033132. Glyco_hydro_1_N_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03356. BGL. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q25BW5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSAAKLPKSF VWGYATAAYQ IEGSPDKDGR EPSIWDTFCK APGKIADGSS
    60 70 80 90 100
    GDVATDSYNR WREDVQLLKS YGVKAYRFSL SWSRIIPKGG RSDPVNGAGI
    110 120 130 140 150
    KHYRTLIEEL VKEGITPFVT LYHWDLPQAL DDRYGGWLNK EEAIQDFTNY
    160 170 180 190 200
    AKLCFESFGD LVQNWITFNE PWVISVMGYG NGIFAPGHVS NTEPWIVSHH
    210 220 230 240 250
    IILAHAHAVK LYRDEFKEKQ GGQIGITLDS HWLIPYDDTD ASKEATLRAM
    260 270 280 290 300
    EFKLGRFANP IYKGEYPPRI KKILGDRLPE FTPEEIELVK GSSDFFGLNT
    310 320 330 340 350
    YTTHLVQDGG SDELAGFVKT GHTRADGTQL GTQSDMGWLQ TYGPGFRWLL
    360 370 380 390 400
    NYLWKAYDKP VYVTENGFPV KGENDLPVEQ AVDDTDRQAY YRDYTEALLQ
    410 420 430 440 450
    AVTEDGADVR GYFGWSLLDN FEWAEGYKVR FGVTHVDYET QKRTPKKSAE
    460
    FLSRWFKEHI EE
    Length:462
    Mass (Da):52,599
    Last modified:April 18, 2006 - v1
    Checksum:i25C864094A8F5007
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB253326 mRNA. Translation: BAE87008.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB253326 mRNA. Translation: BAE87008.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2E3ZX-ray1.50A/B1-462[»]
    2E40X-ray1.90A/B1-462[»]
    ProteinModelPortaliQ25BW5.
    SMRiQ25BW5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1A_PHACH.

    Proteomic databases

    PRIDEiQ25BW5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 1380.
    SABIO-RKQ25BW5.

    Miscellaneous databases

    EvolutionaryTraceiQ25BW5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR033132. Glyco_hydro_1_N_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03356. BGL. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBGL1A_PHACH
    AccessioniPrimary (citable) accession number: Q25BW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: April 18, 2006
    Last modified: November 2, 2016
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.