Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-glucosidase 1A

Gene

BGL1A

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Kineticsi

  1. KM=6.80 mM for cellobiose2 Publications
  2. KM=0.229 mM for p-nitrophenyl-beta-D-glucoside (pNP-Glu)2 Publications
  3. KM=10.2 mM for p-nitrophenyl-beta-D-galactoside (pNP-Gal)2 Publications
  4. KM=0.752 mM for p-nitrophenyl-beta-D-xyloside (pNP-Xyl)2 Publications

    pH dependencei

    Optimum pH is 6.0-6.5.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201Substrate1 Publication
    Binding sitei123 – 1231Substrate1 Publication
    Binding sitei169 – 1691Substrate1 Publication
    Active sitei170 – 1701Proton donor1 Publication
    Binding sitei301 – 3011Substrate1 Publication
    Active sitei365 – 3651Nucleophile1 Publication
    Binding sitei415 – 4151Substrate1 Publication

    GO - Molecular functioni

    • beta-glucosidase activity Source: UniProtKB
    • cellobiose glucosidase activity Source: UniProtKB

    GO - Biological processi

    • cellulose catabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 1380.
    SABIO-RKQ25BW5.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1A_PHACH.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 1A1 PublicationImported (EC:3.2.1.21)
    Alternative name(s):
    Cellobiase 1A1 Publication
    Gene namesi
    Name:BGL1AImported
    OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
    Taxonomic identifieri5306 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi173 – 1731V → C: 2-fold decrease in affinity for cellobiose. 1 Publication
    Mutagenesisi177 – 1771M → L: Small decrease in affinity for cellobiose. 1 Publication
    Mutagenesisi229 – 2291D → N: 17-fold decrease in affinity for cellobiose and displays more acidic optimum pH than wild-type. No effect on optimum pH; when associated with A-253. 1 Publication
    Mutagenesisi231 – 2311H → D: 3-fold decrease in affinity for cellobiose. 1 Publication
    Mutagenesisi253 – 2531K → A: 7-fold decrease in affinity for cellobiose. No effect on optimum pH; when associated with N-229. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Beta-glucosidase 1APRO_0000390787Add
    BLAST

    Proteomic databases

    PRIDEiQ25BW5.

    Expressioni

    Inductioni

    Expressed constitutively in cellobiose and glucose cultures.1 Publication

    Structurei

    Secondary structure

    1
    462
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi11 – 155Combined sources
    Helixi18 – 214Combined sources
    Helixi27 – 293Combined sources
    Helixi34 – 385Combined sources
    Beta strandi51 – 533Combined sources
    Turni57 – 604Combined sources
    Helixi61 – 7010Combined sources
    Beta strandi74 – 796Combined sources
    Helixi82 – 854Combined sources
    Helixi97 – 11317Combined sources
    Beta strandi116 – 1249Combined sources
    Helixi128 – 1347Combined sources
    Helixi136 – 1383Combined sources
    Helixi140 – 15819Combined sources
    Turni159 – 1613Combined sources
    Beta strandi164 – 1696Combined sources
    Helixi171 – 1799Combined sources
    Helixi193 – 21523Combined sources
    Helixi217 – 2204Combined sources
    Beta strandi223 – 2297Combined sources
    Beta strandi232 – 2398Combined sources
    Helixi240 – 25314Combined sources
    Helixi255 – 2639Combined sources
    Helixi268 – 2747Combined sources
    Helixi275 – 2773Combined sources
    Helixi283 – 2897Combined sources
    Beta strandi294 – 2996Combined sources
    Beta strandi303 – 3086Combined sources
    Helixi313 – 3153Combined sources
    Beta strandi317 – 3237Combined sources
    Beta strandi329 – 3313Combined sources
    Beta strandi333 – 3364Combined sources
    Helixi343 – 35715Combined sources
    Beta strandi361 – 3666Combined sources
    Helixi373 – 3753Combined sources
    Helixi378 – 3814Combined sources
    Helixi385 – 40319Combined sources
    Beta strandi409 – 4157Combined sources
    Helixi423 – 4253Combined sources
    Beta strandi433 – 4364Combined sources
    Turni438 – 4403Combined sources
    Beta strandi443 – 4453Combined sources
    Helixi447 – 45913Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E3ZX-ray1.50A/B1-462[»]
    2E40X-ray1.90A/B1-462[»]
    ProteinModelPortaliQ25BW5.
    SMRiQ25BW5. Positions 3-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ25BW5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni422 – 4232Substrate binding

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03356. BGL. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q25BW5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSAAKLPKSF VWGYATAAYQ IEGSPDKDGR EPSIWDTFCK APGKIADGSS
    60 70 80 90 100
    GDVATDSYNR WREDVQLLKS YGVKAYRFSL SWSRIIPKGG RSDPVNGAGI
    110 120 130 140 150
    KHYRTLIEEL VKEGITPFVT LYHWDLPQAL DDRYGGWLNK EEAIQDFTNY
    160 170 180 190 200
    AKLCFESFGD LVQNWITFNE PWVISVMGYG NGIFAPGHVS NTEPWIVSHH
    210 220 230 240 250
    IILAHAHAVK LYRDEFKEKQ GGQIGITLDS HWLIPYDDTD ASKEATLRAM
    260 270 280 290 300
    EFKLGRFANP IYKGEYPPRI KKILGDRLPE FTPEEIELVK GSSDFFGLNT
    310 320 330 340 350
    YTTHLVQDGG SDELAGFVKT GHTRADGTQL GTQSDMGWLQ TYGPGFRWLL
    360 370 380 390 400
    NYLWKAYDKP VYVTENGFPV KGENDLPVEQ AVDDTDRQAY YRDYTEALLQ
    410 420 430 440 450
    AVTEDGADVR GYFGWSLLDN FEWAEGYKVR FGVTHVDYET QKRTPKKSAE
    460
    FLSRWFKEHI EE
    Length:462
    Mass (Da):52,599
    Last modified:April 18, 2006 - v1
    Checksum:i25C864094A8F5007
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB253326 mRNA. Translation: BAE87008.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB253326 mRNA. Translation: BAE87008.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E3ZX-ray1.50A/B1-462[»]
    2E40X-ray1.90A/B1-462[»]
    ProteinModelPortaliQ25BW5.
    SMRiQ25BW5. Positions 3-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1A_PHACH.

    Proteomic databases

    PRIDEiQ25BW5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 1380.
    SABIO-RKQ25BW5.

    Miscellaneous databases

    EvolutionaryTraceiQ25BW5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03356. BGL. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium."
      Tsukada T., Igarashi K., Yoshida M., Samejima M.
      Appl. Microbiol. Biotechnol. 73:807-814(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
      Strain: K-3Imported.
      Tissue: Mycelium1 Publication.
    2. "Role of subsite +1 residues in pH dependence and catalytic activity of the glycoside hydrolase family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium."
      Tsukada T., Igarashi K., Fushinobu S., Samejima M.
      Biotechnol. Bioeng. 99:1295-1302(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF VAL-173; MET-177; ASP-229; HIS-231 AND LYS-253.
      Strain: K-31 Publication.
      Tissue: Mycelium1 Publication.
    3. "Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium."
      Nijikken Y., Tsukada T., Igarashi K., Samejima M., Wakagi T., Shoun H., Fushinobu S.
      FEBS Lett. 581:1514-1520(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH SUBSTRATE.
      Strain: K-31 Publication.
      Tissue: Mycelium1 Publication.

    Entry informationi

    Entry nameiBGL1A_PHACH
    AccessioniPrimary (citable) accession number: Q25BW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: April 18, 2006
    Last modified: April 1, 2015
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.