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Protein

Beta-glucosidase 1B

Gene

BGL1B

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Kineticsi

  1. KM=0.218 mM for cellobiose2 Publications
  2. KM=0.619 mM for p-nitrophenyl-beta-D-glucoside (pNP-Glu)2 Publications
  3. KM=4.02 mM for p-nitrophenyl-beta-D-galactoside (pNP-Gal)2 Publications
  4. KM=1.07 mM for cellobionolactone2 Publications

    pH dependencei

    Optimum pH is 6.0-6.5.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251SubstrateBy similarity
    Binding sitei128 – 1281SubstrateBy similarity
    Binding sitei174 – 1741SubstrateBy similarity
    Active sitei175 – 1751Proton donorBy similarity
    Binding sitei316 – 3161SubstrateBy similarity
    Active sitei380 – 3801NucleophilePROSITE-ProRule annotationBy similarity
    Binding sitei430 – 4301SubstrateBy similarity

    GO - Molecular functioni

    • cellobiose glucosidase activity Source: UniProtKB

    GO - Biological processi

    • cellulose catabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 1380.
    SABIO-RKQ25BW4.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1B_PHACH.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 1B1 PublicationImported (EC:3.2.1.21)
    Alternative name(s):
    Cellobiase 1B1 Publication
    Gene namesi
    Name:BGL1BImported
    OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
    Taxonomic identifieri5306 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 540540Beta-glucosidase 1BPRO_0000390788Add
    BLAST

    Proteomic databases

    PRIDEiQ25BW4.

    Expressioni

    Inductioni

    Expressed in cellobiose culture, but repressed in glucose culture.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi5306.JGI99870.

    Structurei

    3D structure databases

    ProteinModelPortaliQ25BW4.
    SMRiQ25BW4. Positions 10-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni437 – 4382Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG2723.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q25BW4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSASAAPPNK LPADFLWGFA TASFQIEGAT DVDGRGKSIW DDFSKIPGKT
    60 70 80 90 100
    LDGKNGDVAT DSYNRWREDV DLLVQYGVKS YRFSISWSRI IPLGGRNDPV
    110 120 130 140 150
    NEAGIKFYSD LIDALLERGI VPFVTLYHWD LPQALHDRYL GWLNKDEIVQ
    160 170 180 190 200
    DYVRYAGVCF ERFGDRVKHW LTMNEPWCIS ILGYGRGVFA PGRSSDRMRS
    210 220 230 240 250
    PEGDSSTEPW IVGHSVILAH AYAVKLYREQ FKANRGGQIG ITLNGDWAMP
    260 270 280 290 300
    YDDSPQNIEA AQHALDVAIG WFADPIYLGQ YPAYMKEMLG DRLPEFTPEE
    310 320 330 340 350
    LAVVKGSSDF YGMNTYTTNL CKAGGEDEFQ GNVEYTFTRP DGTQLGTAAH
    360 370 380 390 400
    CSWLQDYAPG FRDLLNYLYK RYRKPIYVTE NGFAVKDENS KPLEEALKDD
    410 420 430 440 450
    DRVHYYQGVT DSLLAAVKED GVDVRGYFGW SLLDNFEWAD GYITRFGVTY
    460 470 480 490 500
    VDYDTQKRYP KDSGKFLSQW FPAHIAESPK PAAETKKAAT PSPLKPHGAI
    510 520 530 540
    SNGVSKKSSA TKEPKSASRK KGRKAPFARF TAYISAFLGL
    Length:540
    Mass (Da):60,665
    Last modified:April 18, 2006 - v1
    Checksum:iD0B4518C9A11C071
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB253327 mRNA. Translation: BAE87009.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB253327 mRNA. Translation: BAE87009.1.

    3D structure databases

    ProteinModelPortaliQ25BW4.
    SMRiQ25BW4. Positions 10-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi5306.JGI99870.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1B_PHACH.

    Proteomic databases

    PRIDEiQ25BW4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    eggNOGiCOG2723.

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 1380.
    SABIO-RKQ25BW4.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium."
      Tsukada T., Igarashi K., Yoshida M., Samejima M.
      Appl. Microbiol. Biotechnol. 73:807-814(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
      Strain: K-3Imported.
      Tissue: Mycelium1 Publication.
    2. "Role of subsite +1 residues in pH dependence and catalytic activity of the glycoside hydrolase family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium."
      Tsukada T., Igarashi K., Fushinobu S., Samejima M.
      Biotechnol. Bioeng. 99:1295-1302(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K-31 Publication.
      Tissue: Mycelium1 Publication.

    Entry informationi

    Entry nameiBGL1B_PHACH
    AccessioniPrimary (citable) accession number: Q25BW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: April 18, 2006
    Last modified: April 1, 2015
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.