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Q25BW4

- BGL1B_PHACH

UniProt

Q25BW4 - BGL1B_PHACH

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Protein

Beta-glucosidase 1B

Gene

BGL1B

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Kineticsi

  1. KM=0.218 mM for cellobiose2 Publications
  2. KM=0.619 mM for p-nitrophenyl-beta-D-glucoside (pNP-Glu)2 Publications
  3. KM=4.02 mM for p-nitrophenyl-beta-D-galactoside (pNP-Gal)2 Publications
  4. KM=1.07 mM for cellobionolactone2 Publications

pH dependencei

Optimum pH is 6.0-6.5.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251SubstrateBy similarity
Binding sitei128 – 1281SubstrateBy similarity
Binding sitei174 – 1741SubstrateBy similarity
Active sitei175 – 1751Proton donorBy similarity
Binding sitei316 – 3161SubstrateBy similarity
Active sitei380 – 3801NucleophileBy similarityPROSITE-ProRule annotation
Binding sitei430 – 4301SubstrateBy similarity

GO - Molecular functioni

  1. cellobiose glucosidase activity Source: UniProtKB

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.21. 4722.
SABIO-RKQ25BW4.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.
mycoCLAPiBGL1B_PHACH.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 1BImported1 Publication (EC:3.2.1.21)
Alternative name(s):
Cellobiase 1B1 Publication
Gene namesi
Name:BGL1BImported
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesCorticialesCorticiaceaePhanerochaete

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540Beta-glucosidase 1BPRO_0000390788Add
BLAST

Proteomic databases

PRIDEiQ25BW4.

Expressioni

Inductioni

Expressed in cellobiose culture, but repressed in glucose culture.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi5306.JGI99870.

Structurei

3D structure databases

ProteinModelPortaliQ25BW4.
SMRiQ25BW4. Positions 10-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni437 – 4382Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG2723.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q25BW4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSASAAPPNK LPADFLWGFA TASFQIEGAT DVDGRGKSIW DDFSKIPGKT
60 70 80 90 100
LDGKNGDVAT DSYNRWREDV DLLVQYGVKS YRFSISWSRI IPLGGRNDPV
110 120 130 140 150
NEAGIKFYSD LIDALLERGI VPFVTLYHWD LPQALHDRYL GWLNKDEIVQ
160 170 180 190 200
DYVRYAGVCF ERFGDRVKHW LTMNEPWCIS ILGYGRGVFA PGRSSDRMRS
210 220 230 240 250
PEGDSSTEPW IVGHSVILAH AYAVKLYREQ FKANRGGQIG ITLNGDWAMP
260 270 280 290 300
YDDSPQNIEA AQHALDVAIG WFADPIYLGQ YPAYMKEMLG DRLPEFTPEE
310 320 330 340 350
LAVVKGSSDF YGMNTYTTNL CKAGGEDEFQ GNVEYTFTRP DGTQLGTAAH
360 370 380 390 400
CSWLQDYAPG FRDLLNYLYK RYRKPIYVTE NGFAVKDENS KPLEEALKDD
410 420 430 440 450
DRVHYYQGVT DSLLAAVKED GVDVRGYFGW SLLDNFEWAD GYITRFGVTY
460 470 480 490 500
VDYDTQKRYP KDSGKFLSQW FPAHIAESPK PAAETKKAAT PSPLKPHGAI
510 520 530 540
SNGVSKKSSA TKEPKSASRK KGRKAPFARF TAYISAFLGL
Length:540
Mass (Da):60,665
Last modified:April 18, 2006 - v1
Checksum:iD0B4518C9A11C071
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB253327 mRNA. Translation: BAE87009.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB253327 mRNA. Translation: BAE87009.1 .

3D structure databases

ProteinModelPortali Q25BW4.
SMRi Q25BW4. Positions 10-477.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5306.JGI99870.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.
mycoCLAPi BGL1B_PHACH.

Proteomic databases

PRIDEi Q25BW4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG2723.

Enzyme and pathway databases

BRENDAi 3.2.1.21. 4722.
SABIO-RK Q25BW4.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium."
    Tsukada T., Igarashi K., Yoshida M., Samejima M.
    Appl. Microbiol. Biotechnol. 73:807-814(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
    Strain: K-3Imported.
    Tissue: Mycelium1 Publication.
  2. "Role of subsite +1 residues in pH dependence and catalytic activity of the glycoside hydrolase family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium."
    Tsukada T., Igarashi K., Fushinobu S., Samejima M.
    Biotechnol. Bioeng. 99:1295-1302(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: K-31 Publication.
    Tissue: Mycelium1 Publication.

Entry informationi

Entry nameiBGL1B_PHACH
AccessioniPrimary (citable) accession number: Q25BW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: April 18, 2006
Last modified: October 1, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3