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Q25BW4

- BGL1B_PHACH

UniProt

Q25BW4 - BGL1B_PHACH

Protein

Beta-glucosidase 1B

Gene

BGL1B

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

    Kineticsi

    1. KM=0.218 mM for cellobiose2 Publications
    2. KM=0.619 mM for p-nitrophenyl-beta-D-glucoside (pNP-Glu)2 Publications
    3. KM=4.02 mM for p-nitrophenyl-beta-D-galactoside (pNP-Gal)2 Publications
    4. KM=1.07 mM for cellobionolactone2 Publications

    pH dependencei

    Optimum pH is 6.0-6.5.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251SubstrateBy similarity
    Binding sitei128 – 1281SubstrateBy similarity
    Binding sitei174 – 1741SubstrateBy similarity
    Active sitei175 – 1751Proton donorBy similarity
    Binding sitei316 – 3161SubstrateBy similarity
    Active sitei380 – 3801NucleophileBy similarityPROSITE-ProRule annotation
    Binding sitei430 – 4301SubstrateBy similarity

    GO - Molecular functioni

    1. cellobiose glucosidase activity Source: UniProtKB

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BRENDAi3.2.1.21. 4722.
    SABIO-RKQ25BW4.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.
    mycoCLAPiBGL1B_PHACH.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 1BImported1 Publication (EC:3.2.1.21)
    Alternative name(s):
    Cellobiase 1B1 Publication
    Gene namesi
    Name:BGL1BImported
    OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
    Taxonomic identifieri5306 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesCorticialesCorticiaceaePhanerochaete

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 540540Beta-glucosidase 1BPRO_0000390788Add
    BLAST

    Proteomic databases

    PRIDEiQ25BW4.

    Expressioni

    Inductioni

    Expressed in cellobiose culture, but repressed in glucose culture.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi5306.JGI99870.

    Structurei

    3D structure databases

    ProteinModelPortaliQ25BW4.
    SMRiQ25BW4. Positions 10-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni437 – 4382Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG2723.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q25BW4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSASAAPPNK LPADFLWGFA TASFQIEGAT DVDGRGKSIW DDFSKIPGKT    50
    LDGKNGDVAT DSYNRWREDV DLLVQYGVKS YRFSISWSRI IPLGGRNDPV 100
    NEAGIKFYSD LIDALLERGI VPFVTLYHWD LPQALHDRYL GWLNKDEIVQ 150
    DYVRYAGVCF ERFGDRVKHW LTMNEPWCIS ILGYGRGVFA PGRSSDRMRS 200
    PEGDSSTEPW IVGHSVILAH AYAVKLYREQ FKANRGGQIG ITLNGDWAMP 250
    YDDSPQNIEA AQHALDVAIG WFADPIYLGQ YPAYMKEMLG DRLPEFTPEE 300
    LAVVKGSSDF YGMNTYTTNL CKAGGEDEFQ GNVEYTFTRP DGTQLGTAAH 350
    CSWLQDYAPG FRDLLNYLYK RYRKPIYVTE NGFAVKDENS KPLEEALKDD 400
    DRVHYYQGVT DSLLAAVKED GVDVRGYFGW SLLDNFEWAD GYITRFGVTY 450
    VDYDTQKRYP KDSGKFLSQW FPAHIAESPK PAAETKKAAT PSPLKPHGAI 500
    SNGVSKKSSA TKEPKSASRK KGRKAPFARF TAYISAFLGL 540
    Length:540
    Mass (Da):60,665
    Last modified:April 18, 2006 - v1
    Checksum:iD0B4518C9A11C071
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB253327 mRNA. Translation: BAE87009.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB253327 mRNA. Translation: BAE87009.1 .

    3D structure databases

    ProteinModelPortali Q25BW4.
    SMRi Q25BW4. Positions 10-477.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5306.JGI99870.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.
    mycoCLAPi BGL1B_PHACH.

    Proteomic databases

    PRIDEi Q25BW4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG2723.

    Enzyme and pathway databases

    BRENDAi 3.2.1.21. 4722.
    SABIO-RK Q25BW4.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium."
      Tsukada T., Igarashi K., Yoshida M., Samejima M.
      Appl. Microbiol. Biotechnol. 73:807-814(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
      Strain: K-3Imported.
      Tissue: Mycelium1 Publication.
    2. "Role of subsite +1 residues in pH dependence and catalytic activity of the glycoside hydrolase family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium."
      Tsukada T., Igarashi K., Fushinobu S., Samejima M.
      Biotechnol. Bioeng. 99:1295-1302(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K-31 Publication.
      Tissue: Mycelium1 Publication.

    Entry informationi

    Entry nameiBGL1B_PHACH
    AccessioniPrimary (citable) accession number: Q25BW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3