ID   PCKG_CHLFF              Reviewed;         600 AA.
AC   Q256B8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
DE            Short=PEP carboxykinase;
DE            Short=PEPCK;
DE            EC=4.1.1.32;
DE   AltName: Full=Phosphoenolpyruvate carboxylase;
GN   Name=pckG; OrderedLocusNames=CF0098;
OS   Chlamydophila felis (strain Fe/C-56).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae; Chlamydophila.
OX   NCBI_TaxID=264202;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16766509; DOI=10.1093/dnares/dsi027;
RA   Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H.,
RA   Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H.,
RA   Fukushi H., Hattori M., Kuhara S., Shirai M.;
RT   "Genome sequence of the cat pathogen, Chlamydophila felis.";
RL   DNA Res. 13:15-23(2006).
CC   -!- FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to
CC       phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic
CC       pathway that produces glucose from lactate and other precursors
CC       derived from the citric acid cycle (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate
CC       + CO(2).
CC   -!- COFACTOR: Binds 1 manganese ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC       family.
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DR   EMBL; AP006861; BAE80870.1; -; Genomic_DNA.
DR   RefSeq; YP_515015.1; -.
DR   GeneID; 3959148; -.
DR   GenomeReviews; AP006861_GR; CF0098.
DR   KEGG; cfe:CF0098; -.
DR   HOGENOM; Q256B8; -.
DR   OMA; Q256B8; SHPNARF.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) act...; IEA:HAMAP.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:HAMAP.
DR   HAMAP; MF_00452; -; 1.
DR   InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008209; PEP_carboxykinase_GTP.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   Gene3D; G3DSA:3.90.228.20; PEP_carboxykinase_C; 1.
DR   Gene3D; G3DSA:3.40.449.10; PEP_carboxykinase_N; 1.
DR   PANTHER; PTHR11561; PEP_carboxykin; 1.
DR   Pfam; PF00821; PEPCK; 1.
DR   PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR   ProDom; PD004738; PEPCK_N; 1.
DR   PROSITE; PS00505; PEPCK_GTP; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis;
KW   GTP-binding; Lyase; Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    600       Phosphoenolpyruvate carboxykinase [GTP].
FT                                /FTId=PRO_1000080984.
FT   NP_BIND     268    273       GTP (By similarity).
FT   NP_BIND     508    511       GTP (By similarity).
FT   REGION      381    383       Substrate binding (By similarity).
FT   ACT_SITE    269    269       By similarity.
FT   METAL       225    225       Manganese (By similarity).
FT   METAL       245    245       Manganese (By similarity).
FT   METAL       292    292       Manganese (By similarity).
FT   BINDING      76     76       Substrate (By similarity).
FT   BINDING     218    218       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     225    225       Substrate (By similarity).
FT   BINDING     267    267       Substrate (By similarity).
FT   BINDING     383    383       GTP (By similarity).
FT   BINDING     414    414       GTP (By similarity).
SQ   SEQUENCE   600 AA;  66477 MW;  52F3231C978B8B74 CRC64;
     MTSAWSNEIQ HEGLKQWIQE VAELVTPEDI RVCNGSDSEY AEVYSIMQKS GVAIPLNSDL
     HPNCFLVRSS PEDVARVEKF TFICTAKKEE AGPTNNWRDP QEMRQELQGL FRGCMRGRTL
     YVVPFCMGPL NSPFSLIGVE LTDSPYVVCS MKIMTRMGAE VLKSLGTSGT FHKCLHSVGV
     PLSPGEKDVA WPCNPEHMRI VHFQDDSSVM SFGSGYGGNA LLGKKCVALR LASYIARSKN
     WLAEHMLIIG VTNPEGQKKY FAASFPSACG KTNLAMLKPK IPGWKIECIG DDIAWIRPGS
     DGRLYAVNPE YGFFGVAPGT SEKTNPNALA TCRSNSIFTN VALTPDGDVW WEGLTSQPPA
     GLIDWRGNPW QPGGTPAAHP NSRFTTPLQQ CPVLDSQWNS SEGVPLEAII FGGRRSDTIP
     LVYEALSWQH GVTIGASMSS ATTAAIVGEQ GKLRHDPFAM LPFCGYNMAL YFDHWLSFAS
     NASLKLPKIY GVNWFRKDKD GNFIWPGFSE NLRVLEWIFR RTNGEESIAK RTPIGYLPEE
     SSLNLEGLNL SSQALQDLLA VDVPGWLKEV ADVREYCKIF GSDLPQIISD ELFRIERELK
//