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Q256B8 (PCKG_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckG
Ordered Locus Names:CF0098
OrganismChlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]
Taxonomic identifier264202 [NCBI]
Taxonomic lineageBacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452

Subunit structure

Monomer By similarity. HAMAP MF_00452

Subcellular location

Cytoplasm By similarity HAMAP MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452
PRO_1000080984

Regions

Nucleotide binding268 – 2736GTP By similarity
Nucleotide binding508 – 5114GTP By similarity
Region381 – 3833Substrate binding By similarity

Sites

Active site2691 By similarity
Metal binding2251Manganese By similarity
Metal binding2451Manganese By similarity
Metal binding2921Manganese By similarity
Binding site761Substrate By similarity
Binding site2181Substrate; via amide nitrogen By similarity
Binding site2251Substrate By similarity
Binding site2671Substrate By similarity
Binding site3831GTP By similarity
Binding site4141GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q256B8 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 52F3231C978B8B74

FASTA60066,477
        10         20         30         40         50         60 
MTSAWSNEIQ HEGLKQWIQE VAELVTPEDI RVCNGSDSEY AEVYSIMQKS GVAIPLNSDL 

        70         80         90        100        110        120 
HPNCFLVRSS PEDVARVEKF TFICTAKKEE AGPTNNWRDP QEMRQELQGL FRGCMRGRTL 

       130        140        150        160        170        180 
YVVPFCMGPL NSPFSLIGVE LTDSPYVVCS MKIMTRMGAE VLKSLGTSGT FHKCLHSVGV 

       190        200        210        220        230        240 
PLSPGEKDVA WPCNPEHMRI VHFQDDSSVM SFGSGYGGNA LLGKKCVALR LASYIARSKN 

       250        260        270        280        290        300 
WLAEHMLIIG VTNPEGQKKY FAASFPSACG KTNLAMLKPK IPGWKIECIG DDIAWIRPGS 

       310        320        330        340        350        360 
DGRLYAVNPE YGFFGVAPGT SEKTNPNALA TCRSNSIFTN VALTPDGDVW WEGLTSQPPA 

       370        380        390        400        410        420 
GLIDWRGNPW QPGGTPAAHP NSRFTTPLQQ CPVLDSQWNS SEGVPLEAII FGGRRSDTIP 

       430        440        450        460        470        480 
LVYEALSWQH GVTIGASMSS ATTAAIVGEQ GKLRHDPFAM LPFCGYNMAL YFDHWLSFAS 

       490        500        510        520        530        540 
NASLKLPKIY GVNWFRKDKD GNFIWPGFSE NLRVLEWIFR RTNGEESIAK RTPIGYLPEE 

       550        560        570        580        590        600 
SSLNLEGLNL SSQALQDLLA VDVPGWLKEV ADVREYCKIF GSDLPQIISD ELFRIERELK

« Hide

References

[1]"Genome sequence of the cat pathogen, Chlamydophila felis."
Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M.
DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fe/C-56.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006861 Genomic DNA. Translation: BAE80870.1.
RefSeqYP_515015.1. NC_007899.1.

3D structure databases

ProteinModelPortalQ256B8.
SMRQ256B8. Positions 11-600.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ256B8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3959148.
GenomeReviewsGene locus CF0098 in contig AP006861_GR.
KEGGcfe:CF0098.
PATRIC20203746. VBIChlFel51660_0102.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHBG484437.
OMACGYHMGD.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycCFEL264202:CF0098-MONOMER.

Family and domain databases

HAMAPMF_00452. PEPCK_GTP.
[Tree]
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KOK01596.
PANTHERPTHR11561. PEP_carboxykin. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_CHLFF
AccessionPrimary (citable) accession number: Q256B8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 18, 2006
Last modified: January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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