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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Chlamydophila felis (strain Fe/C-56)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761SubstrateUniRule annotation
Binding sitei218 – 2181Substrate; via amide nitrogenUniRule annotation
Metal bindingi225 – 2251ManganeseUniRule annotation
Binding sitei225 – 2251SubstrateUniRule annotation
Metal bindingi245 – 2451Manganese; via tele nitrogenUniRule annotation
Binding sitei267 – 2671SubstrateUniRule annotation
Active sitei269 – 2691UniRule annotation
Metal bindingi292 – 2921ManganeseUniRule annotation
Binding sitei383 – 3831GTPUniRule annotation
Binding sitei414 – 4141GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2736GTPUniRule annotation
Nucleotide bindingi508 – 5114GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCFEL264202:GJCG-101-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:CF0098
OrganismiChlamydophila felis (strain Fe/C-56)
Taxonomic identifieri264202 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
ProteomesiUP000001260 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080984Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264202.CF0098.

Structurei

3D structure databases

ProteinModelPortaliQ256B8.
SMRiQ256B8. Positions 11-600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 3833Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q256B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSAWSNEIQ HEGLKQWIQE VAELVTPEDI RVCNGSDSEY AEVYSIMQKS
60 70 80 90 100
GVAIPLNSDL HPNCFLVRSS PEDVARVEKF TFICTAKKEE AGPTNNWRDP
110 120 130 140 150
QEMRQELQGL FRGCMRGRTL YVVPFCMGPL NSPFSLIGVE LTDSPYVVCS
160 170 180 190 200
MKIMTRMGAE VLKSLGTSGT FHKCLHSVGV PLSPGEKDVA WPCNPEHMRI
210 220 230 240 250
VHFQDDSSVM SFGSGYGGNA LLGKKCVALR LASYIARSKN WLAEHMLIIG
260 270 280 290 300
VTNPEGQKKY FAASFPSACG KTNLAMLKPK IPGWKIECIG DDIAWIRPGS
310 320 330 340 350
DGRLYAVNPE YGFFGVAPGT SEKTNPNALA TCRSNSIFTN VALTPDGDVW
360 370 380 390 400
WEGLTSQPPA GLIDWRGNPW QPGGTPAAHP NSRFTTPLQQ CPVLDSQWNS
410 420 430 440 450
SEGVPLEAII FGGRRSDTIP LVYEALSWQH GVTIGASMSS ATTAAIVGEQ
460 470 480 490 500
GKLRHDPFAM LPFCGYNMAL YFDHWLSFAS NASLKLPKIY GVNWFRKDKD
510 520 530 540 550
GNFIWPGFSE NLRVLEWIFR RTNGEESIAK RTPIGYLPEE SSLNLEGLNL
560 570 580 590 600
SSQALQDLLA VDVPGWLKEV ADVREYCKIF GSDLPQIISD ELFRIERELK
Length:600
Mass (Da):66,477
Last modified:April 18, 2006 - v1
Checksum:i52F3231C978B8B74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006861 Genomic DNA. Translation: BAE80870.1.
RefSeqiYP_515015.1. NC_007899.1.

Genome annotation databases

KEGGicfe:CF0098.
PATRICi20203746. VBIChlFel51660_0102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006861 Genomic DNA. Translation: BAE80870.1.
RefSeqiYP_515015.1. NC_007899.1.

3D structure databases

ProteinModelPortaliQ256B8.
SMRiQ256B8. Positions 11-600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264202.CF0098.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGicfe:CF0098.
PATRICi20203746. VBIChlFel51660_0102.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciCFEL264202:GJCG-101-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fe/C-56.

Entry informationi

Entry nameiPCKG_CHLFF
AccessioniPrimary (citable) accession number: Q256B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 18, 2006
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.