Q255W7 (MURD_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	CF0149

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257177
Regions
	Nucleotide binding	109 – 115	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q255W7 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: DB7119E7EB30DD30	FASTA	419	46,307
	10 20 30 40 50 60 MNHQRVVVLG AGVTGKSAAE FLHKKGDFVI GIDGSWDALI SCNFFHQRYL DKTENFPEDV 70 80 90 100 110 120 DLFVRSPGIK TSHPLVIEAK RRDIPIVTDV QLAFQSPEFY QYPSLGITGS TGKTTTVLFL 130 140 150 160 170 180 VHLLHSLGIS AFAMGNIGFP ILQAMYQKGV RVVEISSFQL TEQEQKIPVL SGAAILNISE 190 200 210 220 230 240 NHLDYHQTLH AYSEAKMNIA KCLQWPDSLW SGEGVSSGRS YLEYTEEIDS VLDKGGALKP 250 260 270 280 290 300 LYLHDRNNYC AAYALAKEVT SVPLEAFLQA VQTFEKPPHR IEYLGEKDGV RYINDSKATT 310 320 330 340 350 360 MSSVEKALMA VKENVIVIMG GRNKESNFTS LIPVLTQTVK HIVAMGECRK EIAQALSSSL 370 380 390 400 410 PLTQARDLQE AVSIAQSIAQ PGDVILLSPG CASFDQFRSF EERGDCFRQL VGDMEALKI

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fe/C-56.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006861 Genomic DNA. Translation: BAE80921.1.
RefSeq	YP_515066.1. NC_007899.1.
3D structure databases
ProteinModelPortal	Q255W7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q255W7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3958963.
GenomeReviews	Gene locus CF0149 in contig AP006861_GR.
KEGG	cfe:CF0149.
PATRIC	20203862. VBIChlFel51660_0155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	LERHKTL.
PhylomeDB	Q255W7.
ProtClustDB	PRK00683.
Enzyme and pathway databases
BioCyc	CFEL264202:CF0149-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLFF

Accession

Primary (citable) accession number: Q255W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program