Reviewed, UniProtKB/Swiss-Prot Q255W7 (MURD_CHLFF)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	CF0149

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257177
Regions
	Nucleotide binding	109 – 115	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q255W7-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: DB7119E7EB30DD30	FASTA	419	46,307
	10 20 30 40 50 60 MNHQRVVVLG AGVTGKSAAE FLHKKGDFVI GIDGSWDALI SCNFFHQRYL DKTENFPEDV 70 80 90 100 110 120 DLFVRSPGIK TSHPLVIEAK RRDIPIVTDV QLAFQSPEFY QYPSLGITGS TGKTTTVLFL 130 140 150 160 170 180 VHLLHSLGIS AFAMGNIGFP ILQAMYQKGV RVVEISSFQL TEQEQKIPVL SGAAILNISE 190 200 210 220 230 240 NHLDYHQTLH AYSEAKMNIA KCLQWPDSLW SGEGVSSGRS YLEYTEEIDS VLDKGGALKP 250 260 270 280 290 300 LYLHDRNNYC AAYALAKEVT SVPLEAFLQA VQTFEKPPHR IEYLGEKDGV RYINDSKATT 310 320 330 340 350 360 MSSVEKALMA VKENVIVIMG GRNKESNFTS LIPVLTQTVK HIVAMGECRK EIAQALSSSL 370 380 390 400 410 PLTQARDLQE AVSIAQSIAQ PGDVILLSPG CASFDQFRSF EERGDCFRQL VGDMEALKI

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006861 Genomic DNA. Translation: BAE80921.1.
RefSeq	YP_515066.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3958963.
GenomeReviews	Gene locus CF0149 in contig AP006861_GR.
KEGG	cfe:CF0149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q255W7.
OMA	Q255W7. DSKATNF.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLFF

Accession

Primary (citable) accession number: Q255W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)