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Q255M7 (TRMB_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CF0239
OrganismChlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]
Taxonomic identifier264202 [NCBI]
Taxonomic lineageBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288135

Sites

Active site1291 By similarity
Binding site541S-adenosyl-L-methionine By similarity
Binding site791S-adenosyl-L-methionine By similarity
Binding site1061S-adenosyl-L-methionine By similarity
Binding site1291S-adenosyl-L-methionine By similarity
Binding site1331Substrate By similarity
Binding site1651Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q255M7 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 956CA60F7DB4DA16

FASTA22426,838
        10         20         30         40         50         60 
MKPQDLKVPF FWEERSTKIK DNVLYVPEHY FKHHQFIMPS WEEFFGNDNP ISCELCSGNG 

        70         80         90        100        110        120 
DWVVAQAKET PEVNWIAVEK RFDRVRKIWS KMHNCRVTNL RIVCGDAQTF FLHYLGEAVV 

       130        140        150        160        170        180 
QRIIVNFPDP WPKSRHRKHR LFQDEFLNNV VRVLTEPGIL ILATDDKNYL LQAIQIMRQY 

       190        200        210        220 
LSPTMEDPYY CEVENYGNSW FETLWRSKGR EIFYTEFVKK LGYS 

« Hide

References

[1]"Genome sequence of the cat pathogen, Chlamydophila felis."
Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M.
DNA Res. 13:15-23(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fe/C-56.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006861 Genomic DNA. Translation: BAE81011.1.
RefSeqYP_515156.1. NC_007899.1.

3D structure databases

ProteinModelPortalQ255M7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264202.CF0239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3958670.
KEGGcfe:CF0239.
PATRIC20204049. VBIChlFel51660_0247.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000034940.
KOK03439.
OMAIVELAMN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycCFEL264202:GJCG-248-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CHLFF
AccessionPrimary (citable) accession number: Q255M7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways