Reviewed, UniProtKB/Swiss-Prot Q255M5 (MURB_CHLFF)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CF0241

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_1000002877
Regions
	Domain	26 – 191	166	FAD-binding PCMH-type
Sites
	Active site	170	1	By similarity
	Active site	218	1	Proton donor By similarity
	Active site	287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q255M5-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: B3296719151C5334	FASTA	296	32,538
	10 20 30 40 50 60 MKESTVIHFP FSVRRGVWLS KYSTFRIGGP ANYFKEVNSA EEAQQVIQFL YSQNYPFIIV 70 80 90 100 110 120 GKGSNCLFDD QGFDGFVLYN NIQKKEFLSE TTIKVYSGMS FSFLGKTLSS SGYSGLEFAV 130 140 150 160 170 180 GIPGSVGGAV FMNAGIGNQD IASAIESVEA INSNGDIISY QAAELEFGYR RSRFQNSKEF 190 200 210 220 230 240 ILSATFRLSK SASSIQIAKD LLQNKLLSQP YQQPSAGCIF RNPPGNYAGK LIDEAGLKGL 250 260 270 280 290 SLGGAQISSK HANFIVNNGR ATSHEVKELI QIVRDKLKSQ GISLEEEVRI IPYRLP

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006861 Genomic DNA. Translation: BAE81013.1.
RefSeq	YP_515158.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q255M5.
Genome annotation databases
GeneID	3958962.
GenomeReviews	Gene locus CF0241 in contig AP006861_GR.
KEGG	cfe:CF0241.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q255M5.
OMA	KITFIND.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_CHLFF

Accession

Primary (citable) accession number: Q255M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)