Reviewed, UniProtKB/Swiss-Prot Q255G0 (DAPA_CHLFF)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	CF0306

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050174
Sites
	Active site	157	1	Schiff-base intermediate with substrate By similarity
	Site	129	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q255G0-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: D3AE12788269CA09	FASTA	290	32,314
	10 20 30 40 50 60 MKLLTASVTP FLPNYDIDFL SVEKLLHSQE KEGNGVVLLG STGESLALTV QEKEKLVAYA 70 80 90 100 110 120 CSLDLKIPII VGVPGTSLHE ASAWVSLCQS YPVDGFLITS PIYTKPGIQG QILWFESILN 130 140 150 160 170 180 ITNKPAILYN IPSRAGSPIY LETVRALSGH PFFYGIKDSG GSIDRCREYT QVCPNLIIYC 190 200 210 220 230 240 GDDGLWPQMH QCGARGLISV LSNSWPKEAH NYVEDPFNKN NSLLWCELVS WINQTTNPIS 250 260 270 280 290 IKAMLAYKQD IAYDILRLPL SIKDLQNKKV LPVLVEKMSQ WSQICECVFT

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006861 Genomic DNA. Translation: BAE81078.1.
RefSeq	YP_515223.1.
3D structure databases
SMR	Q255G0. Positions 3-279.
ModBase	Search...
Protein-protein interaction databases
STRING	Q255G0.
Genome annotation databases
GeneID	3958683.
GenomeReviews	Gene locus CF0306 in contig AP006861_GR.
KEGG	cfe:CF0306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	CMLYNVP.
Enzyme and pathway databases
BioCyc	CFEL264202:CF0306-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. Dihydrodipicolinate_synth. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CHLFF

Accession

Primary (citable) accession number: Q255G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 18, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)