Q255E6 (RPOB_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	CF0320

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length	1252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1252	1252	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300297

Sequences

	Sequence		Length	Mass (Da)
	Q255E6 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: 8ECCF64157D73154	FASTA	1,252	140,346
	10 20 30 40 50 60 MFKCPERVSV KKKEDILDLP NLIEIQIKSY KQFLQIGKLA EERDNVGLEE VFREIFPIKS 70 80 90 100 110 120 YNEATILEYL SYNLGVPKYS PEECIRRGIT YSVTLKVRFR LTDETGIKEE EVYMGTIPIM 130 140 150 160 170 180 TDKGTFIING AERVVVSQVH RSPGINFEQE KHSKGNILFS FRIIPYRGSW LEAIFDINDL 190 200 210 220 230 240 IYIHIDRKKR RRKILAMTFI RALGYSSDAD IIEEFFQIEE CSLKSEKDFS VLVGKILADN 250 260 270 280 290 300 VLDEASSLVY GKAGEKLSTA MLKRMLDADI STLKIALEAD ENHPIIKMLA KDPTDSYEAA 310 320 330 340 350 360 LKDFYRRLRP GEPATLANAR STIMRLFFDP KRYNLGRVGR YKLNRKLGFP MDEESLSQVT 370 380 390 400 410 420 LRKEDVIGAL KYLIRLKMGD EKASIDDIDH LANRRVRSVG ELIQNQCRSG LARMEKIVRE 430 440 450 460 470 480 RMNLFDFSSD TLIPGKIISA KGLTSVLKDF FGRSQLSQFM DQTNPVAELT HKRRLSALGP 490 500 510 520 530 540 GGLNRERAGF EVRDVHASHY GRICPIETPE GPNIGLITSL SSFAKINEFG FIETPYRIVR 550 560 570 580 590 600 DGVVTDEIEY MTADVEEECV IAQASANLDE YNMFTDPVCW ARYRGEAFEA DTSTVTHMDV 610 620 630 640 650 660 SPKQLVSIVT GLIPFLEHDD ANRALMGSNM QRQAVPLLKT EAPIVGTGLE ARAAKDSGAI 670 680 690 700 710 720 VVAEEDGVVE YVDGYKVVVA AKHNPTLKRT YEFKKFLRSN SGTCINQRPL CSVGDIVVKG 730 740 750 760 770 780 DVIADGPATD QGELALGKNI LVAFMPWYGY NFEDAVIISE KLIKQDAYTS IYIEEFELTA 790 800 810 820 830 840 RDTKLGKEEI TRDIPNVSEE VLANLGEDGI IRIGAEVKPG DILVGKITPK SETELAPEER 850 860 870 880 890 900 LLRAIFGEKA ADVKDASLTV PPGTEGVVMD VKVFSRKDRL SKSDDELVEE AVHLKDLQKG 910 920 930 940 950 960 YKNQISVLKT EYREKLGALL LNEKAPASII HRRTADILVQ EGTVFDQETI ELLEQESLVD 970 980 990 1000 1010 1020 LLMPPCDMYD VLKNLLSDYE TSLQRLEVNY KTEVEHIREG DADLDHGVIR QVKVYVASKR 1030 1040 1050 1060 1070 1080 KLQVGDKMAG RHGNKGVVSK IVPEADMPYL ANGETIQMIL NPLGVPSRMN LGQVLETHLG 1090 1100 1110 1120 1130 1140 YAAKTAGIHV KTPVFEGFPE SRIWDMMIEQ GLPADGKSYL YDGKTGERFD NTVVIGYIYM 1150 1160 1170 1180 1190 1200 LKLSHLIADK IHARSIGPYS LVTQQPLGGK AQMGGQRFGE MEVWALEAYG VAHMLQEILT 1210 1220 1230 1240 1250 VKSDDVSGRT RIYESIVKGE NLLKSGTPES FNVLIKEMQG LGLDVRPMVV DA

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fe/C-56.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006861 Genomic DNA. Translation: BAE81092.1.
RefSeq	YP_515237.1. NC_007899.1.
3D structure databases
HSSP	HSSP built from PDB template 2GHO based on UniProtKB Q9KWU7.
ProteinModelPortal	Q255E6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q255E6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3958734.
GenomeReviews	Gene locus CF0320 in contig AP006861_GR.
KEGG	cfe:CF0320.
PATRIC	20204230. VBIChlFel51660_0331.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	IGRNKTY.
PhylomeDB	Q255E6.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	CFEL264202:CF0320-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_CHLFF

Accession

Primary (citable) accession number: Q255E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program