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Q255B1 (TRMD_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:CF0355
OrganismChlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]
Taxonomic identifier264202 [NCBI]
Taxonomic lineageBacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer By similarity. HAMAP MF_00605

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_0000257402

Regions

Region129 – 1346S-adenosyl-L-methionine binding By similarity

Sites

Binding site1091S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q255B1 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: 6C5F1BB0A683BF69

FASTA35540,380
        10         20         30         40         50         60 
MEIDILSLFP DYFDSPLRSS ILGRAIKNGL LKIQSRDIRE FGLGKWKQVD DAPFNNDGML 

        70         80         90        100        110        120 
LMAEPVVQAI RYVKRRESRV IHLSPQGVPL TAQKSRELAK CSHLIFLCGH YEGIDERALE 

       130        140        150        160        170        180 
IEVDEEISIG DYVLTNGGIA ALVVIDALSR FIPGVLGNQE SAEKDSLENG LLEGPQYTRP 

       190        200        210        220        230        240 
RVFEGREVPQ VLLQGDHQAI ARWRKQVSLD RTRERRPDLY VRYLYDRESE DVPQMEADPK 

       250        260        270        280        290        300 
QSAFEGECAV VLDVENINRS KRFYSKVFKS NQPIGDKLYI PGKTQMVLHL QEVGLKNKNT 

       310        320        330        340        350 
AVLSLRLDCE NDFYSFLGRW KMLGGTLEQA DDRGAVRLVR DFDGHLWAIS CKKAK

« Hide

References

[1]"Genome sequence of the cat pathogen, Chlamydophila felis."
Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M.
DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fe/C-56.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006861 Genomic DNA. Translation: BAE81127.1.
RefSeqYP_515272.1. NC_007899.1.

3D structure databases

ProteinModelPortalQ255B1.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ255B1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3958574.
GenomeReviewsGene locus CF0355 in contig AP006861_GR.
KEGGcfe:CF0355.
PATRIC20204300. VBIChlFel51660_0366.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHBG285805.
OMARYLYDRE.
PhylomeDBQ255B1.
ProtClustDBPRK01037.

Enzyme and pathway databases

BioCycCFEL264202:CF0355-MONOMER.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00088. TrmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_CHLFF
AccessionPrimary (citable) accession number: Q255B1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 18, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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