Q25566 (UMPS_NAEGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine 5'-monophosphate synthase
Short name=UMP synthase

Including the following 2 domains:

1. Orotate phosphoribosyltransferase
   Short name=OPRTase
   EC=2.4.2.10
2. Orotidine 5'-phosphate decarboxylase
   EC=4.1.1.23
   Alternative name(s):
   OMPdecase

Gene names

Name:

UMP

Organism

Naegleria gruberi (Amoeba)

Taxonomic identifier

5762 [NCBI]

Taxonomic lineage

Eukaryota › Heterolobosea › Schizopyrenida › Vahlkampfiidae › Naegleria

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: InterPro 'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Uridine 5'-monophosphate synthase		PRO_0000139655
Sites
	Active site	322	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q25566 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: ECE04004A92B0080	FASTA	494	55,809
	10 20 30 40 50 60 METTTIDIQA LKKELVLDLI QIGALKFGRF TLKSGIVSPF YVDLRIIGPK MLRQFSVLLY 70 80 90 100 110 120 HVMTVKGLTN MEKRVICGVP YSALTFSSCM SMTYDLPMVI CRKERKQYGT GNMVEGIYTK 130 140 150 160 170 180 NETNCILIED VITSGASIVE TAEKLENEGL LVTDALVFLT REQLPLTNGM HILKKGEKVY 190 200 210 220 230 240 NVHPCLTMTE VTQVLLDEGK MSQEQREDIL QFIGTNTFSE TKTTAVPQKK KELTFTERAE 250 260 270 280 290 300 LTNNEFSKKL FKLMEEKQTN LCVAADITSK DDLLKLADET GPEICMLKTH IDTLDDQPDE 310 320 330 340 350 360 QFTQQLKELA KKHNFLIFED RKLADIGQVV KQQYARGPFK IAQWSDLCNS HLVSGGSATV 370 380 390 400 410 420 KALKESLKEE NITEPRGLLL IAQMSTEGAT TGESTKQEAL KVALENPDFV SGFICQSKLR 430 440 450 460 470 480 DDLDQFLYCT PGVRLDVKGD SLGQQYNSPE YVVCEKKCDV IIVGRGIYHD KERQNAAKLY 490 RKLGWEAYQK RIQQ

References

[1]	"A bifunctional UMP synthase gene from Naegleria gruberi expresses OMP decarboxylase activity in bacteria and in yeast." Remillard S.P., Lai E.Y., Fulton C. Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NEG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08073 Genomic DNA. Translation: AAA29385.1.
3D structure databases
ProteinModelPortal	Q25566.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR001754. OMPdeCOase_dom. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR01740. PyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. False negative. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UMPS_NAEGR

Accession

Primary (citable) accession number: Q25566

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)