Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uridine 5'-monophosphate synthase

Gene

UMP

Organism
Naegleria gruberi (Amoeba)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
Orotidine 5'-phosphate = UMP + CO2.

Pathway:iUMP biosynthesis via de novo pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes UMP from orotate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Uridine 5'-monophosphate synthase (UMP)
  2. Uridine 5'-monophosphate synthase (UMP)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei322 – 3221By similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Decarboxylase, Glycosyltransferase, Lyase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
UPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine 5'-monophosphate synthase
Short name:
UMP synthase
Including the following 2 domains:
Orotate phosphoribosyltransferase (EC:2.4.2.10)
Short name:
OPRTase
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMPdecase
Gene namesi
Name:UMP
OrganismiNaegleria gruberi (Amoeba)
Taxonomic identifieri5762 [NCBI]
Taxonomic lineageiEukaryotaHeteroloboseaSchizopyrenidaVahlkampfiidaeNaegleria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Uridine 5'-monophosphate synthasePRO_0000139655Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5762.XP_002673182.1.

Structurei

3D structure databases

ProteinModelPortaliQ25566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
In the C-terminal section; belongs to the OMP decarboxylase family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q25566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METTTIDIQA LKKELVLDLI QIGALKFGRF TLKSGIVSPF YVDLRIIGPK
60 70 80 90 100
MLRQFSVLLY HVMTVKGLTN MEKRVICGVP YSALTFSSCM SMTYDLPMVI
110 120 130 140 150
CRKERKQYGT GNMVEGIYTK NETNCILIED VITSGASIVE TAEKLENEGL
160 170 180 190 200
LVTDALVFLT REQLPLTNGM HILKKGEKVY NVHPCLTMTE VTQVLLDEGK
210 220 230 240 250
MSQEQREDIL QFIGTNTFSE TKTTAVPQKK KELTFTERAE LTNNEFSKKL
260 270 280 290 300
FKLMEEKQTN LCVAADITSK DDLLKLADET GPEICMLKTH IDTLDDQPDE
310 320 330 340 350
QFTQQLKELA KKHNFLIFED RKLADIGQVV KQQYARGPFK IAQWSDLCNS
360 370 380 390 400
HLVSGGSATV KALKESLKEE NITEPRGLLL IAQMSTEGAT TGESTKQEAL
410 420 430 440 450
KVALENPDFV SGFICQSKLR DDLDQFLYCT PGVRLDVKGD SLGQQYNSPE
460 470 480 490
YVVCEKKCDV IIVGRGIYHD KERQNAAKLY RKLGWEAYQK RIQQ
Length:494
Mass (Da):55,809
Last modified:November 1, 1996 - v1
Checksum:iECE04004A92B0080
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08073 Genomic DNA. Translation: AAA29385.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08073 Genomic DNA. Translation: AAA29385.1.

3D structure databases

ProteinModelPortaliQ25566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5762.XP_002673182.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
UPA00070; UER00120.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A bifunctional UMP synthase gene from Naegleria gruberi expresses OMP decarboxylase activity in bacteria and in yeast."
    Remillard S.P., Lai E.Y., Fulton C.
    Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NEG.

Entry informationi

Entry nameiUMPS_NAEGR
AccessioniPrimary (citable) accession number: Q25566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.