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Q25566 (UMPS_NAEGR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uridine 5'-monophosphate synthase

Short name=UMP synthase

Including the following 2 domains:

  1. Orotate phosphoribosyltransferase
    Short name=OPRTase
    EC=2.4.2.10
  2. Orotidine 5'-phosphate decarboxylase
    EC=4.1.1.23
    Alternative name(s):
    OMPdecase
Gene names
Name:UMP
OrganismNaegleria gruberi (Amoeba)
Taxonomic identifier5762 [NCBI]
Taxonomic lineageEukaryotaHeteroloboseaSchizopyrenidaVahlkampfiidaeNaegleria

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01208

Orotidine 5'-phosphate = UMP + CO2. HAMAP-Rule MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP-Rule MF_01208

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

Sequence similarities

In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

In the C-terminal section; belongs to the OMP decarboxylase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Uridine 5'-monophosphate synthase HAMAP-Rule MF_01208
PRO_0000139655

Sites

Active site3221 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q25566 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: ECE04004A92B0080

FASTA49455,809
        10         20         30         40         50         60 
METTTIDIQA LKKELVLDLI QIGALKFGRF TLKSGIVSPF YVDLRIIGPK MLRQFSVLLY 

        70         80         90        100        110        120 
HVMTVKGLTN MEKRVICGVP YSALTFSSCM SMTYDLPMVI CRKERKQYGT GNMVEGIYTK 

       130        140        150        160        170        180 
NETNCILIED VITSGASIVE TAEKLENEGL LVTDALVFLT REQLPLTNGM HILKKGEKVY 

       190        200        210        220        230        240 
NVHPCLTMTE VTQVLLDEGK MSQEQREDIL QFIGTNTFSE TKTTAVPQKK KELTFTERAE 

       250        260        270        280        290        300 
LTNNEFSKKL FKLMEEKQTN LCVAADITSK DDLLKLADET GPEICMLKTH IDTLDDQPDE 

       310        320        330        340        350        360 
QFTQQLKELA KKHNFLIFED RKLADIGQVV KQQYARGPFK IAQWSDLCNS HLVSGGSATV 

       370        380        390        400        410        420 
KALKESLKEE NITEPRGLLL IAQMSTEGAT TGESTKQEAL KVALENPDFV SGFICQSKLR 

       430        440        450        460        470        480 
DDLDQFLYCT PGVRLDVKGD SLGQQYNSPE YVVCEKKCDV IIVGRGIYHD KERQNAAKLY 

       490 
RKLGWEAYQK RIQQ 

« Hide

References

[1]"A bifunctional UMP synthase gene from Naegleria gruberi expresses OMP decarboxylase activity in bacteria and in yeast."
Remillard S.P., Lai E.Y., Fulton C.
Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NEG.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08073 Genomic DNA. Translation: AAA29385.1.

3D structure databases

ProteinModelPortalQ25566.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00070; UER00119.
UPA00070; UER00120.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPMF_01208. PyrE.
InterProIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01740. pyrF. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUMPS_NAEGR
AccessionPrimary (citable) accession number: Q25566
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways