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Q254X5 (SYL_CHLFF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CF0391
OrganismChlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]
Taxonomic identifier264202 [NCBI]
Taxonomic lineageBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila

Protein attributes

Sequence length820 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 820820Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009321

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif601 – 6055"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6041ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q254X5 [UniParc].

Last modified April 18, 2006. Version 1.
Checksum: EAC547EDE26B1A2C

FASTA82094,247
        10         20         30         40         50         60 
MRYDPSLIEK KWQEFWKEHK SFKANEADDK PKYYVLDMFP YPSGAGLHVG HLIGYTATDI 

        70         80         90        100        110        120 
VARYKRAKGF SVLHPMGWDS FGLPAEQYAV RTGTHPRETT QKNIENFRKQ LSAMGFSYDE 

       130        140        150        160        170        180 
GREFATSDPE YYRWTQKLFL LLYEKGLAYM ADMAVNYCPE LGTVLSNEEV ENGLSIEGGY 

       190        200        210        220        230        240 
PVERRMLRQW ILRITAYSDQ LLEGLEGLDW PENVKQLQRN WIGKSEGALV RFKVNNQNFL 

       250        260        270        280        290        300 
EVFTTRPDTL CGVSFLVIAP EHPEVGQLIA EDRREEVEAY ICRAQSKSER DRISESKIKS 

       310        320        330        340        350        360 
GVFTGTYAKH PITGADIPIW VSDYVILGYG SGVVMGVPAH DERDREFAET FSLPIYEVLD 

       370        380        390        400        410        420 
TDGYCIHSNH GDFLLDGLWG REAQDYVIAY LQKKNLGESK VAYKLRDWLF SRQRYWGEPI 

       430        440        450        460        470        480 
PIIHFEDGTC RPLEDDELPL LPPEIQDYRP EGFGQGPLAK VKEWVDIHDK KTNRQGRRET 

       490        500        510        520        530        540 
HTMPQWAGSC WYYLRFCDAL NSQAPWSNEK EKYWMPVDLY IGGAEHAVLH LLYSRFWHRV 

       550        560        570        580        590        600 
FYDAGMVSTP EPFKKLINQG LVLATSYRIP GKGYVYPEDA HEDNGVWMST SGEELEVRQE 

       610        620        630        640        650        660 
KMSKSKLNGV DPQILIEEFG ADALRMYAMF SGPLDKNKLW CNQGVSGCRR FLNRFYELVT 

       670        680        690        700        710        720 
SSLVQDIDDP KGMYLAHRLV HRVGEDIEKM SLNTIPSSFM EFINEFVKLD IYPKSALAMV 

       730        740        750        760        770        780 
VRALAPIAPH ISEELWTILG NAPGIDQAGW PEVDPKYLED TSVTFVIQVN GKLRARLDIS 

       790        800        810        820 
KNAAREEVLS LAREAVSRYL EDKEVKKEIF VPNRLVNFVL 

« Hide

References

[1]"Genome sequence of the cat pathogen, Chlamydophila felis."
Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M.
DNA Res. 13:15-23(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fe/C-56.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006861 Genomic DNA. Translation: BAE81163.1.
RefSeqYP_515308.1. NC_007899.1.

3D structure databases

ProteinModelPortalQ254X5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264202.CF0391.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3958432.
KEGGcfe:CF0391.
PATRIC20204382. VBIChlFel51660_0406.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCFEL264202:GJCG-407-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CHLFF
AccessionPrimary (citable) accession number: Q254X5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 18, 2006
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries