Reviewed, UniProtKB/Swiss-Prot Q254V9 (PYRG_CHLFF)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	CF0407

Organism

Chlamydophila felis (strain Fe/C-56) [Complete proteome] [HAMAP]

Taxonomic identifier

264202 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	CTP synthase HAMAP MF_01227		PRO_0000266092
Regions
	Domain	294 – 533	240	Glutamine amidotransferase type-1
	Region	1 – 255	255	Aminator domain HAMAP MF_01227
Sites
	Active site	380	1	Nucleophile By similarity
	Active site	506	1	By similarity
	Active site	508	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q254V9-1 [UniParc]. Last modified April 18, 2006. Version 1. Checksum: C0C15A926AB660B6	FASTA	539	60,092
	10 20 30 40 50 60 MSFKCIFLTG GVVSSLGKGL TAASLALLLE RQSLKVSMLK LDPYLNVDPG TMNPYEHGEV 70 80 90 100 110 120 YVTNDGIETD LDLGHYHRFS SVKLSKYSTA TSGQIYARVI KKEREGVYLG STVQVIPHIT 130 140 150 160 170 180 NEIIEVILEC ARENQPDVLI VEIGGTVGDI ESLPFLEAIR QFRYEHAENC FSIHMTYVPY 190 200 210 220 230 240 LKAAGEVKTK PTQHSVQSLR SIGIIPDAIL CRSESSLSPD VKKKISLFCN VPNNAVFNVV 250 260 270 280 290 300 DIEHSIYEMP LMLSQENIST FITEKLGLFT KQEDLSDWKT LVERLRNPSL DKVRIGLVGK 310 320 330 340 350 360 YVQHKDAYKS VFEALTHAAL SLNSSVEILP LDSEDPHFLE TLEQCDGCLV PGGFGSRGWE 370 380 390 400 410 420 GKITAAKLCR ERGIPYFGIC LGMQVLVVEY ARNVLRLEKA NSTEMDAETP DPVICMMDGQ 430 440 450 460 470 480 ASLVATGGTM RLGAYPCLIS PGTKVHEAYG ESEVMERHRH RYEVNFDYVQ QFKDFGLNVV 490 500 510 520 530 GTCPQQGLCE IVEIENHPWM IGVQFHPEFL SKLIAPHPLF VGFIQAAILY SRNKSYVQA

References

[1]

"Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23(2006) [PubMed: 16766509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006861 Genomic DNA. Translation: BAE81179.1.
RefSeq	YP_515324.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3958889.
GenomeReviews	Gene locus CF0407 in contig AP006861_GR.
KEGG	cfe:CF0407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q254V9.
OMA	Q254V9. EFNNAYR.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_CHLFF

Accession

Primary (citable) accession number: Q254V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)